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  4. Pig Endothelial nitric oxide synthase,eNOS ELISA Kit

Pig Endothelial nitric oxide synthase,eNOS ELISA Kit

  • 中文名称：
    猪内皮型一氧化氮合成酶(eNOS)酶联免疫试剂盒
  • 货号：
    CSB-E08574p
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Pig NOS3 ELISA Kit was designed for the quantitative measurement of Pig NOS3 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 3.12 IU/mL-200 IU/mL and the sensitivity is 0.78 IU/mL.

  • 别名：
    NOS3 ELISA Kit; NOS ELISA Kit; Nitric oxide synthase ELISA Kit; endothelial ELISA Kit; EC 1.14.13.39 ELISA Kit; Constitutive NOS ELISA Kit; cNOS ELISA Kit; EC-NOS ELISA Kit; Endothelial NOS ELISA Kit; eNOS ELISA Kit; NOS type III ELISA Kit; NOSIII ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Sus scrofa (Pig)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    3.12 IU/mL-200 IU/mL
  • 灵敏度：
    0.78 IU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig eNOS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %83
    Range %80-86
    1:2Average %97
    Range %93-100
    1:4Average %84
    Range %80-88
    1:8Average %104
    Range %100-108
  • 回收率：
    The recovery of pig eNOS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8985-92
    EDTA plasma (n=4)10097-103
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    IU/mlOD1OD2AverageCorrected
    2002.589 2.547 2.568 2.386
    1001.829 1.809 1.819 1.637
    501.064 1.087 1.076 0.894
    250.643 0.657 0.650 0.468
    12.50.450 0.468 0.459 0.277
    6.250.358 0.369 0.364 0.182
    3.120.274 0.298 0.286 0.104
    00.181 0.183 0.182
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
  • 基因功能参考文献：
    1. NOS3 was lowest in kidneys removed from live pigs, greater in kidneys from pigs with brain death, and greatest in kidneys from pigs with cardiac arrest. PMID: 25380860
    2. Rapid atrial pacing increases ADMA and down-regulates eNOS expression in an ADMA-independent manner. PMID: 20926145
    3. Icariin and icariside II may increase the eNOS expression through activating EGF-EGFR pathway in porcine aortic endothelial cells. PMID: 21844953
    4. Data suggest that pig sperm contain bNOS, iNOS, and eNOS; up-regulation of NOS by leptin during acrosome reaction and inhibition of acrosome reaction by inhibitors of nitric oxide synthases suggests these enzymes are involved in acrosome reaction. PMID: 21970701
    5. Periodic acceleration (pGz) acutely increases endothelial and neuronal nitric oxide synthase expression in endomyocardium of normal swine. PMID: 19022311
    6. Data suggest that angiotensin II regulates nNOS and eNOS expression and NOS activity in afferent arterioles of the developing kidney via angiotensin 1 and 2 receptors. PMID: 20386492
    7. Endothelial nitric oxide synthase mRNA expression was elevated in gestational day 50 intrauterine growth retardation placenta and areola compared to gd50 control. PMID: 20381143
    8. Oligonol prevented the impairment of eNOS activity induced by high glucose through reversing altered eNOS phosphorylation status. PMID: 20128797
    9. Exercise training significantly increased total eNOS and phosphorylated levels of eNOS (pSer(1179)) in collateral-dependent arteries of experimental minipigs. PMID: 20363881
    10. Data show that wall shear stress increases with a decrease in artery diameter; eNOS protein contents decrease with an increase in diameter. PMID: 19432590
    11. The inhibitory domain of eNOs stimulates vasodilation of the pulmonary artery. PMID: 14729513
    12. endothelium-derived NO stimulates guanylyl cyclase in vascular smooth muscle cells and, thereby, permits CO to cause dilation by activating K(Ca) channels PMID: 15191891
    13. the p38 MAPK is an upstream component of Akt-mediated eNOS activation PMID: 15333638
    14. Ritonavir significantly impairs vasomotor function and reduces eNOS expression in porcine coronary artery endothelial cells. PMID: 15905730
    15. AMPK activity is essential for estradiol-induced eNOS activation in vascular endothelial cells via the promotion of eNOS interaction with HSP90 PMID: 15967841
    16. Hsp90 is a modulator of eNOS activity and vascular reactivity in the newborn piglet pulmonary circulation PMID: 17337508
    17. Glomerular eNOS gene expression was studied during postnatal maturation and AT1 receptor inhibition. PMID: 17437130
    18. in the aortic arch, nitric oxide synthase 3 endothelial (eNOS) was downregulated, compared with the thoracic aorta PMID: 17588585
    19. eNOS phosphorylation at Ser-116, therefore, appears to contribute to negative modulation of eNOS activity and hence to regulation of vascular tone. PMID: 17822962
    20. Analysis of the 2.1 kb 5' flanking region reveals that the swine eNOS promoter is, like its counterparts in human and other species, a TATA-less promoter; the proximal region from -227 to -82 is necessary for eNOS basal promoter activity. PMID: 17852356
    21. regenerated coronary endothelial cells exhibit downregulation of eNOS PMID: 17942849
    22. Immunoreactivity of eNOS was similar to NADPH-d staining. Clear iNOS immunoreactivity was detected in the luminal epithelium, endometrial stroma and individual endometrial glands. PMID: 18165177
    23. Low-fat diet and chronic exercise preserve endothelial function in the pig penis by sustaining active eNOS in its dimeric form and by limiting eNOS interaction with its negative regulator caveolin-1. PMID: 18194184
    24. Effects of chronic NOS3 inhibition on endothelium-dependent and -independent relaxation in arteries that perfuse skeletal muscle of swine are reported. PMID: 18568942
    25. The increase in NOS protein seen in both the endothelium and vascular smooth muscle in response to cerebral vasospasm is enhanced by dopamine in a D(2)R-dependent mechanism PMID: 18807216
    26. results suggest that endothelial (eNOS) and inducible (iNOS) nitric oxide synthase are responsible for regulation of blood circulation in umbilical cord PMID: 18992077
    27. Report effect of uncoupling endothelial nitric oxide synthase on calcium homeostasis in aged porcine endothelial cells. PMID: 19176602
    28. Low expressions of eNOS3 and Ve-cadherin in the salvaged sub-healthy microvascular endothelium of infarcted and marginal areas suggest that endothelial system is impaired at 7-day of reperfused acute myocardial infarction. PMID: 19519369
    29. ENOS activation during cardioplegic arrest occurs through phosphorylation at Ser1177 mediated by Akt/PKB. PMID: 19795322

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  • 亚细胞定位：
    Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Cell membrane.
  • 蛋白家族：
    NOS family
  • 数据库链接：