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  4. Pig Creatine kinase M-type(CKM) ELISA kit

Pig Creatine kinase M-type(CKM) ELISA kit

  • 中文名称：
    猪M型肌酸激酶(CKM)酶联免疫试剂盒
  • 货号：
    CSB-EL005459PI
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Pig CKM ELISA Kit was designed for the quantitative measurement of Pig CKM protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 mU/mL-10 mU/mL and the sensitivity is 0.039 mU/mL.

  • 别名：
    CKMCreatine kinase M-type ELISA Kit; EC 2.7.3.2 ELISA Kit; Creatine kinase M chain ELISA Kit; Creatine phosphokinase M-type ELISA Kit; CPK-M ELISA Kit; M-CK ELISA Kit
  • 缩写：
    CKM
  • Uniprot No.：
  • 种属：
    Sus scrofa (Pig)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.156 mU/mL-10 mU/mL
  • 灵敏度：
    0.039 mU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig CKM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:5 Average % 103
    Range % 92-107
    1:10 Average % 98
    Range % 91-102
    1:20 Average % 93
    Range % 84-97
    1:40 Average % 99
    Range % 90-103
  • 回收率：
    The recovery of pig CKM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 100 88-103
    EDTA plasma (n=4) 92 84-96
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mU/ml OD1 OD2 Average Corrected
    10 2.059 1.993 2.026 1.891
    5 1.557 1.688 1.623 1.488
    2.5 1.081 1.128 1.105 0.970
    1.25 0.622 0.637 0.630 0.495
    0.625 0.323 0.331 0.327 0.192
    0.312 0.215 0.222 0.219 0.084
    0.156 0.179 0.173 0.176 0.041
    0 0.138 0.132 0.135 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
  • 基因功能参考文献：
    1. this study examined the heterogeneity of creatine kinase (CK) forward flux rate of hearts with postinfarction left ventricular (LV) remodeling. PMID: 27606901
  • 蛋白家族：
    ATP:guanido phosphotransferase family
  • 数据库链接：

    KEGG: ssc:397264

    UniGene: Ssc.415