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Pig Corticoliberin(CRH) ELISA kit

  • 中文名称：
    猪促肾上腺皮质素释放素(CRH)酶联免疫试剂盒
  • 货号：
    CSB-EL005963PI
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Pig CRH ELISA Kit was designed for the quantitative measurement of Pig CRH protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 100 pg/mL-1600 pg/mL and the sensitivity is 100 pg/mL.

  • 别名：
    CRHCorticoliberin ELISA kit; Corticotropin-releasing factor ELISA kit; CRF ELISA kit; Corticotropin-releasing hormone ELISA kit
  • 缩写：
    CRH
  • Uniprot No.：
  • 种属：
    Sus scrofa (Pig)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    100 pg/mL-1600 pg/mL
  • 灵敏度：
    100 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig CRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 90  
    Range % 85-95  
    1:2 Average % 95  
    Range % 92-101  
    1:4 Average % 82  
    Range % 80-83  
    1:8 Average % 88  
    Range % 85-92  
  • 回收率：
    The recovery of pig CRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 93 87-97  
    EDTA plasma (n=4) 94 90-98  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    1600 0.196 0.184 0.190    
    800 0.322 0.343 0.333    
    400 0.516 0.510 0.513    
    200 1.037 1.100 1.069    
    100 2.158 2.323 2.241    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Hormone regulating the release of corticotropin from pituitary gland. Induces NLRP6 in intestinal epithelial cells, hence may influence gut microbiota profile.
  • 基因功能参考文献：
    1. Evidence is provided for porcine corticotropin releasing hormone (CRH) as a quantitative trait locus (QTL) affecting growth and body composition. (corticotropin releasing hormone; CRH; CRF) PMID: 16483545
    2. CRH inhibits local progesterone production from luteal cells in swine corpus lutem. PMID: 20431270
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Sauvagine/corticotropin-releasing factor/urotensin I family
  • 组织特异性：
    Produced by the hypothalamus.
  • 数据库链接：