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  4. Pig Colipase(CLPS) ELISA kit

Pig Colipase(CLPS) ELISA kit

  • 中文名称：
    猪辅脂酶(CLPS)酶联免疫试剂盒
  • 货号：
    CSB-EL005578PI
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Pig CLPS ELISA Kit was designed for the quantitative measurement of Pig CLPS protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 mU/mL-1000 mU/mL and the sensitivity is 3.9 mU/mL.

  • 别名：
    CLPS ELISA Kit; Colipase ELISA Kit; Procolipase II ELISA Kit
  • 缩写：
    CLPS
  • Uniprot No.：
  • 种属：
    Sus scrofa (Pig)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 mU/mL-1000 mU/mL
  • 灵敏度：
    3.9 mU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig CLPS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 87
    Range % 82-92
    1:2 Average % 99
    Range % 94-103
    1:4 Average % 103
    Range % 98-107
    1:8 Average % 95
    Range % 90-101
  • 回收率：
    The recovery of pig CLPS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 90 85-105
    EDTA plasma (n=4) 104 88-111
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mU/ml OD1 OD2 Average Corrected
    1000 2.297 2.312 2.305 2.196
    500 1.786 1.688 1.737 1.628
    250 1.133 1.202 1.168 1.059
    125 0.654 0.665 0.660 0.551
    62.5 0.389 0.374 0.382 0.273
    31.2 0.242 0.254 0.248 0.139
    15.6 0.177 0.187 0.182 0.073
    0 0.105 0.113 0.109 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Colipase is a cofactor of pancreatic lipase. It allows the lipase to anchor itself to the lipid-water interface. Without colipase the enzyme is washed off by bile salts, which have an inhibitory effect on the lipase.; Enterostatin has a biological activity as a satiety signal.
  • 基因功能参考文献：
    1. isotropic orientation of colipase which could explain its incapacity to reverse the inhibitory effects of these lipids on the lipase activity PMID: 18052211
    2. Analysis of structures of NMR colipase models and simulations of their interactions with various lipid aggregates, lipid droplet, and bile salt micelle, were carried out to determine and to map the lipid binding sites on colipase. PMID: 18506778
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Colipase family
  • 组织特异性：
    Expressed by the pancreas.
  • 数据库链接：

    KEGG: ssc:397407

    STRING: 9823.ENSSSCP00000001686

    UniGene: Ssc.514