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Pig Carboxypeptidase B(CPB1) ELISA kit

  • 中文名称:
    猪羧肽酶B(CPB1)酶联免疫试剂盒
  • 货号:
    CSB-EL005883PI
  • 规格:
    96T/48T
  • 价格:
    ¥4200/¥3000
  • 其他:

产品详情

  • 产品描述:

    This Pig CPB1 ELISA Kit was designed for the quantitative measurement of Pig CPB1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

  • 别名:
    CPB1 ELISA Kit; CPBCarboxypeptidase B ELISA Kit; EC 3.4.17.2 ELISA Kit
  • 缩写:
    CPB1
  • Uniprot No.:
  • 种属:
    Sus scrofa (Pig)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    0.312 ng/mL-20 ng/mL
  • 灵敏度:
    0.078 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Metabolism
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of pig CPB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %89
    Range %82-95
    1:2Average %92
    Range %85-100
    1:4Average %94
    Range %86-102
    1:8Average %95
    Range %90-100
  • 回收率:
    The recovery of pig CPB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-99
    EDTA plasma (n=4)9590-101
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    202.627 2.647 2.637 2.551
    102.444 2.431 2.438 2.352
    51.977 1.985 1.981 1.895
    2.51.432 1.526 1.479 1.393
    1.250.905 0.942 0.924 0.838
    0.6250.595 0.635 0.615 0.529
    0.3120.340 0.343 0.342 0.256
    00.085 0.087 0.086  
  • 数据处理:
  • 货期:
    3-5 working days

产品评价

靶点详情

  • 基因功能参考文献:
    1. Its catalytically important Tyr248 residue is oriented and hydrogen-bonded to solvent water molecules, and locates the furthest away from the catalytic zinc ion as compared to previous structures. PMID: 19802820
  • 亚细胞定位:
    Secreted. Zymogen granule lumen.
  • 蛋白家族:
    Peptidase M14 family
  • 数据库链接: