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Pig Brain-derived neurotrophic factor(BDNF) ELISA kit

  • 中文名称：
    猪脑源性神经营养因子(BDNF)酶联免疫试剂盒
  • 货号：
    CSB-EL002655PI
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Pig BDNF ELISA Kit was designed for the quantitative measurement of Pig BDNF protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 9.375 ng/mL-600 ng/mL and the sensitivity is 2.35 ng/mL.

  • 别名：
    BDNFBrain-derived neurotrophic factor ELISA kit; BDNF) [Cleaved into: BDNF precursor form ELISA kit; ProBDNF)] ELISA kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Sus scrofa (Pig)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    9.375 ng/mL-600 ng/mL
  • 灵敏度：
    2.35 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig BDNF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 96
    Range % 91-102
    1:2 Average % 102
    Range % 97-107
    1:4 Average % 109
    Range % 105-113
    1:8 Average % 103
    Range % 96-107
  • 回收率：
    The recovery of pig BDNF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 102 98-105
    EDTA plasma (n=4) 86 93-92
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    600 2.134 2.081 2.108 2.016
    300 1.537 1.623 1.580 1.488
    150 1.167 1.123 1.145 1.053
    75 0.769 0.774 0.772 0.680
    37.5 0.513 0.503 0.508 0.416
    18.75 0.353 0.344 0.349 0.257
    9.375 0.227 0.221 0.224 0.132
    0 0.091 0.093 0.092 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Important signaling molecule that activates signaling cascades downstream of NTRK2. During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.; Important signaling molecule that activates signaling cascades downstream of NTRK2. Activates signaling cascades via the heterodimeric receptor formed by NGFR and SORCS2. Signaling via NGFR and SORCS2 plays a role in synaptic plasticity and long-term depression (LTD). Binding to NGFR and SORCS2 promotes neuronal apoptosis. Promotes neuronal growth cone collapse.
  • 基因功能参考文献：
    1. measurement of brain derived neurotrophic factor in serum brings a practical approach to study the effects of environmental enrichment on neurobiological changes in domestic animals PMID: 30007130
    2. Lf upregulated several canonical signaling pathways associated with neurodevelopment and cognition and influenced ~10 genes involved in the brain-derived neurotrophin factor (BDNF) signaling pathway. PMID: 25146846
    3. Taken together, these data indicate that recurrent tethering stress in sows over 4.5 years results in a loss of neurotrophic support by BDNF, mediated by an overactive neuroendocrine system. PMID: 22584108
    4. FiO2 used for resuscitation affects matrix metalloproteinases MMP-9 and MMP-2, caspase-3 and BDNF PMID: 21151608
  • 亚细胞定位：
    Secreted.; [BDNF precursor form]: Secreted.
  • 蛋白家族：
    NGF-beta family
  • 组织特异性：
    Brain and central nervous system.
  • 数据库链接：