Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Pig Angiopoietin 2,ANG-2 ELISA Kit

Pig Angiopoietin 2,ANG-2 ELISA Kit

  • 中文名称：
    猪血管生成素2(ANG-2)酶联免疫试剂盒
  • 货号：
    CSB-E08460p
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Pig ANGPT2 ELISA Kit was designed for the quantitative measurement of Pig ANGPT2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.94 ng/mL-60 ng/mL and the sensitivity is 0.235 ng/mL.

  • 别名：
    ANGPT2Angiopoietin-2 ELISA kit; ANG-2 ELISA kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Sus scrofa (Pig)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.94 ng/mL-60 ng/mL
  • 灵敏度：
    0.235 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig ANG-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %86
    Range %83-89
    1:2Average %99
    Range %96-102
    1:4Average %87
    Range %84-90
    1:8Average %97
    Range %91-103
  • 回收率：
    The recovery of pig ANG-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8985-92
    EDTA plasma (n=4)9387-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    602.509 2.596 2.553 2.401
    301.749 1.768 1.759 1.607
    151.004 1.068 1.036 0.884
    7.50.663 0.642 0.653 0.501
    3.750.390 0.378 0.384 0.232
    1.880.298 0.290 0.294 0.142
    0.940.224 0.239 0.232 0.080
    00.151 0.153 0.152
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating angiogenic signals mediated by ANGPT1. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.
  • 基因功能参考文献：
    1. expression of angiopoietin 2 in the porcine metanephric kidney was observed in the podocytes of early developing glomeruli, but not in the cells near the glomerular hilus PMID: 20598355
    2. ANGPT2 remained upregulated during maturation of glomeruli, which may be explained by the continuous growth of the glomeruli, as observed by stereological examination. PMID: 20713984
  • 亚细胞定位：
    Secreted.
  • 数据库链接：

    KEGG: ssc:396730

    STRING: 9823.ENSSSCP00000016694

    UniGene: Ssc.240