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Pig Angiogenin(ANG) ELISA kit

  • 中文名称：
    猪血管生长素(ANG)酶联免疫试剂盒
  • 货号：
    CSB-EL001703PI
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Pig ANG ELISA Kit was designed for the quantitative measurement of Pig ANG protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 94 pg/mL-6000 pg/mL and the sensitivity is 47 pg/mL.

  • 别名：
    ANGAngiogenin ELISA kit; EC 3.1.27.- ELISA kit; Ribonuclease 5 ELISA kit; RNase 5 ELISA kit
  • 缩写：
    ANG
  • Uniprot No.：
  • 种属：
    Sus scrofa (Pig)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    94 pg/mL-6000 pg/mL
  • 灵敏度：
    47 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig ANG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:100 Average % 94  
    Range % 90-98  
    1:200 Average % 96  
    Range % 92-99  
    1:400 Average % 103  
    Range % 97-107  
    1:800 Average % 84  
    Range % 80-88  
  • 回收率：
    The recovery of pig ANG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 90-98  
    EDTA plasma (n=4) 98 94-102  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    6000 0.129 0.128 0.129    
    3000 0.261 0.256 0.259    
    1500 0.509 0.529 0.519    
    750 0.932 0.941 0.937    
    375 1.401 1.394 1.398    
    188 1.753 1.742 1.748    
    94 2.087 2.007 2.047    
    0 2.476 2.554 2.515    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Stimulates ribosomal RNA synthesis including that containing the initiation site sequences of 45S rRNA. Cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs) which inhibit protein synthesis and triggers the assembly of stress granules (SGs). Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo.
  • 亚细胞定位：
    Cytoplasmic vesicle, secretory vesicle lumen. Secreted. Nucleus, nucleolus.
  • 蛋白家族：
    Pancreatic ribonuclease family
  • 数据库链接：