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中文名称:小鼠极低密度脂蛋白(VLDL)酶联免疫试剂盒
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货号:CSB-E17089m
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规格:96T/48T
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价格:¥3600/¥2500
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其他:
产品详情
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产品描述:
This Mouse VLDL ELISA Kit was designed for the quantitative measurement of Mouse VLDL protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.78 ng/mL-50 ng/mL and the sensitivity is 0.195 ng/mL .
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别名:N/A
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缩写:VLDL
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种属:Mus musculus (Mouse)
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样本类型:serum, plasma, tissue homogenates
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检测范围:0.78 ng/mL-50 ng/mL
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灵敏度:0.195 ng/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Cardiovascular
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测定原理:quantitative
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测定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse VLDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:100 Average % 92 Range % 86-98 1:200 Average % 99 Range % 92-106 1:400 Average % 83 Range % 80-85 1:800 Average % 89 Range % 83-97 -
回收率:
The recovery of mouse VLDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 92 87-97 EDTA plasma (n=4) 95 91-98 -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 50 2.158 1.999 2.079 1.994 25 1.344 1.276 1.310 1.225 12.5 0.742 0.704 0.723 0.638 6.25 0.402 0.397 0.400 0.315 3.12 0.274 0.254 0.264 0.179 1.56 0.201 0.196 0.199 0.114 0.78 0.144 0.139 0.142 0.057 0 0.085 0.084 0.085 -
数据处理:
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货期:3-5 working days
引用文献
- Targeted inhibition of PPAR α ameliorates CLA‐induced hypercholesterolemia via hepatic cholesterol biosynthesis reprogramming HY Liu,Liver international,2023
- NPRC deletion mitigated atherosclerosis by inhibiting oxidative stress, inflammation and apoptosis in ApoE knockout mice C Cheng,Signal transduction and targeted therapy,2023
- Therapeutic effect of dichloroacetate against atherosclerosis via hepatic FGF21 induction mediated by acute AMPK activation Min B K, et al,Experimental & Molecular Medicine,2019
- Biochemical mechanism underlying hypertriglyceridemia and hepatic steatosis/hepatomegaly induced by acute schisandrin B treatment in mice Yi Zhang.et al,Health and Disease,2017
产品评价
样品类型:组织匀浆液 肝脏
样品信息:小鼠
稀释比:其他 1:5000
产品评价: 我用 CSB-E17089m检测正常和基因敲除小鼠肝脏VLDL含量,其OD值如图片所示。预期结果和最终检测结果类似,较为准确。
By 唐老师
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