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  4. Mouse tumor necrosis factor-like ligand 1 (TL1) ELISA kit

Mouse tumor necrosis factor-like ligand 1 (TL1) ELISA kit

  • 中文名称：
    小鼠肿瘤坏死因子配体相关分子-1(TL1)酶联免疫试剂盒
  • 货号：
    CSB-E16311m
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    The product CSB-E16311m is a sandwich ELISA kit developed to measure levels of mouse tumor necrosis factor-like ligand 1 (TL1) in serum, plasma, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of TL1 bound in the initial step.

    TL1, encoded by the gene TNFSF15, is a transmembrane protein produced by a variety of cell lines, including endothelial cells, macrophages, dendritic cells (DCs), and T cells. It plays a key role in the regulation and connection between innate immunity and adaptive immunity of mucosal inflammation and thus is implicated in inflammatory bowel disease. It is expressed by immune and endothelial cells in response to numerous pro-inflammatory stimuli. Through its receptor DR3/TNFRSF25, which is expressed primarily on lymphocytes, TL1 stimulates T cell activation, proliferation, and generation of a broad array of cytokines.

  • 别名：
    Tnfsf15 ELISA Kit; Tl1 ELISA Kit; Vegi ELISA Kit; Tumor necrosis factor ligand superfamily member 15 ELISA Kit; TNF ligand-related molecule 1 ELISA Kit; Vascular endothelial cell growth inhibitor ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    62.5 pg/mL-4000 pg/mL
  • 灵敏度：
    15.6 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse TL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 96  
    Range % 90-102  
    1:2 Average % 89  
    Range % 84-95  
    1:4 Average % 95  
    Range % 90-100  
    1:8 Average % 104  
    Range % 100-108  
  • 回收率：
    The recovery of mouse TL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 87 82-93  
    EDTA plasma (n=4) 94 87-98  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    4000 2.871 2.641 2.756 2.601  
    2000 2.086 2.067 2.077 1.922  
    1000 1.348 1.325 1.337 1.182  
    500 0.811 0.826 0.819 0.664  
    250 0.505 0.544 0.525 0.370  
    125 0.293 0.299 0.296 0.141  
    62.5 0.219 0.235 0.227 0.072  
    0 0.154 0.156 0.155 　  
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Receptor for TNFRSF25 and TNFRSF6B. Mediates activation of NF-kappa-B. Inhibits vascular endothelial growth and angiogenesis (in vitro). Promotes activation of caspases and apoptosis. Promotes splenocyte alloactivation.
  • 基因功能参考文献：
    1. TL1A blocking ameliorates intestinal fibrosis in the T cell transfer model of chronic colitis PMID: 29254800
    2. ATF3 protects against LPS-induced acute lung injury by inhibiting TL1A expression. PMID: 28177121
    3. this study shows that TL1A-/- mice are more susceptible to dextran sodium sulfate colitis PMID: 27233964
    4. These data demonstrated a direct role for TL1A-DR3 signaling in tissue fibrosis and that modulation of TL1A-DR3 signaling could inhibit gut fibrosis. PMID: 24850426
    5. TL1A deficiency impacts on the gut microbial composition and the mucosal immune system, especially the intraepithelial TCRgammadelta(+) T-cell subset, and that TL1A is involved in the establishment of adipose tissue. PMID: 25404161
    6. Our data demonstrate a key role for TL1A in promoting ILC2s at mucosal barriers. PMID: 24220298
    7. delivers survival signals to plasma cells and promotes pathogenic Ab production in collagen arthritis PMID: 24140642
    8. TNFSF15 inhibits vasculogenesis by regulating relative levels of membrane-bound and soluble isoforms of VEGF receptor 1. PMID: 23918400
    9. These results establish a novel link between tumor necrosis factor (TNF)-family cytokine TL1A (TNFSF15) and interleukin 13 (IL-13) responses that results in small intestinal inflammation. PMID: 20980995
    10. role of TL1A in mucosal T cells and antigen presenting cells function and showed that up-regulation of TL1A expression can promote mucosal inflammation and gut fibrosis. PMID: 21264313
    11. findings suggest that the transcription factor NF-kappaB plays an important role in the regulation of VEGI expression PMID: 15702971
    12. neonatal CD4+ CD3- cells up-regulate both CD30L and OX40L after adoptive transfer into an adult recipient PMID: 16920944
    13. VEGI may have a key role in both the termination of angiogenesis and facilitation of dendritic cell maturation, which is the initial step in immune responses toward inflammatory stimuli. PMID: 17785811
    14. TL1A may contribute to renal inflammation and injury through DR3-mediated activation of NF-kappaB and caspase-3 PMID: 18287561
    15. Results reveal that TL1A is an important modulator in the development of chronic mucosal inflammation by enhancing T(H)1 and T(H)17 effector functions. PMID: 18598698

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  • 亚细胞定位：
    Membrane; Single-pass type II membrane protein.
  • 蛋白家族：
    Tumor necrosis factor family
  • 数据库链接：