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  4. Mouse tissue inhibitors of metalloproteinase 3,TIMP-3 ELISA kit

Mouse tissue inhibitors of metalloproteinase 3,TIMP-3 ELISA kit

  • 中文名称：
    小鼠基质金属蛋白酶抑制因子3(TIMP-3)酶联免疫试剂盒
  • 货号：
    CSB-E07385m
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse TIMP3 ELISA Kit was designed for the quantitative measurement of Mouse TIMP3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 46.88 pg/mL-3000 pg/mL and the sensitivity is 11.72 pg/mL.

  • 别名：
    Timp3 ELISA Kit; Sun ELISA Kit; Timp-3 ELISA Kit; Metalloproteinase inhibitor 3 ELISA Kit; Tissue inhibitor of metalloproteinases 3 ELISA Kit; TIMP-3 ELISA Kit
  • 缩写：
    TIMP3
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    46.88 pg/mL-3000 pg/mL
  • 灵敏度：
    11.72 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse TIMP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 100
    Range % 95-104
    1:2 Average % 94
    Range % 88-98
    1:4 Average % 98
    Range % 91-102
    1:8 Average % 97
    Range % 90-102
  • 回收率：
    The recovery of mouse TIMP-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 94 87-100
    EDTA plasma (n=4) 101 95-105
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    3000 2.494 2.421 2.458 2.288
    1500 1.893 1.856 1.875 1.705
    750 1.165 1.132 1.149 0.979
    375 0.650 0.643 0.647 0.477
    187.5 0.413 0.390 0.402 0.232
    93.75 0.301 0.294 0.298 0.128
    46.88 0.229 0.225 0.227 0.057
    0 0.169 0.171 0.170 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli.
  • 基因功能参考文献：
    1. Loss of TIMP3 is associated with germinal matrix hemorrhage-intraventricular hemorrhage. PMID: 29456135
    2. altered cortical and trabecular bone mineralization and increased compositional heterogeneity were found in Timp-3 (-/-) bone, all being indicative of high bone remodeling PMID: 28236102
    3. In a clinically relevant CADASIL mouse model, we show that exogenous ADAM17 or HB-EGF restores cerebral arterial tone and blood flow responses, and identify upregulated voltage-dependent potassium channel (KV) number in cerebral arterial myocytes as a heretofore-unrecognized downstream effector of TIMP3-induced deficits. PMID: 27476853
    4. TIMP3 promotes normal microvascular endothelial cell barrier function, at least partially, through inhibition of metalloproteinase-dependent disruption of adherens junctions, and septic downregulation of TIMP3 may contribute to septic MVEC barrier dysfunction. PMID: 26993226
    5. data strongly suggest that TIMP3 has direct neuroprotective effects that can mitigate the deleterious effects associated with TBI, an area with few if any therapeutic options. PMID: 26299440
    6. Elevated levels of TIMP3 and vitronectin, acting downstream of Notch3(ECD) deposition, play a role in CADASIL, producing divergent influences on early CBF deficits and later white matter lesions. PMID: 26648042
    7. 4-Hydroxyisoleucine improved insulin resistant-like state in 3T3-L1 adipocytes by targeting TACE/TIMP3 and the insulin signaling pathway. PMID: 26527864
    8. In a mouse model of prostate cancer, increased tumor growth, proliferation index, increased microvascular density, and invasion was observed in Pten(-/-), Timp3(-/-) prostate tumors compared to Pten(-/-), Timp3(+/+) tumors. PMID: 26332574
    9. Timp3 status determines p53, p38 and Notch coactivation to instruct hepatic cell fate and transformation. PMID: 25347747
    10. lack of TIMP3 increases inflammation and polarizes macrophages towards a more inflammatory phenotype resulting in increased atherosclerosis. PMID: 24943223
    11. TIMP-3 KO mice exhibit enhanced metabolism, as reflected by a higher body temperature than WT mice, possibly due to increased mitochondrial activity. PMID: 24736588
    12. TIMP3 protects kidney from damage PMID: 23760282
    13. TIMP3 reduction is due, at least in part, to increased expression of certain TIMP3-targeting microRNAs in diabetic kidneys compared to healthy controls. PMID: 23797704
    14. Demonstrate a critical role for TIMP3, among all TIMPs, in preserving arterial remodelling in response to Ang II-induced hypertension. PMID: 23524300
    15. TIMP-3 functions to moderate the differentiation of macrophages into proinflammatory (M1) cells. PMID: 23742180
    16. DNA methylation changes were noted in the Timp3 gene during chronic cystitis in a murine model. PMID: 23806407
    17. Loss of Timp3 gene leads to abdominal aortic aneurysm formation in response to angiotensin II. PMID: 23144462
    18. Loss of TIMP3 selectively exacerbates diabetic nephropathy. PMID: 22896043
    19. Pericyte-derived TIMP3 stabilized and ADAMTS1 destabilized the capillary tubular networks PMID: 22383695
    20. we show that TIMP3 produced by the hepatic stroma regulates the basal lymphocyte populations in the liver and prevents autoimmune hepatitis PMID: 22323541
    21. Data suggest that focal ischemia leads to proliferation of immature oligodendrocytes in white matter and that TIMP-3 contributes to a caspase-3-dependent immature OL death via TNF-alpha/TACE-mediated neuroinflammation. PMID: 21871134
    22. macrophage-specific overexpression of TIMP3 protects from metabolic inflammation and related metabolic disorders such as insulin resistance, glucose intolerance, and nonalcoholic steatohepatitis. PMID: 22228717
    23. TIMP3 differentially impacts apoptosis and inflammatory cell influx, based on involvement of TNF, during the process of mammary involution. PMID: 22053204
    24. macrophage-specific overexpression of TIMP3 decreases the inflammatory content and the amplitude of atherosclerotic plaques in low-density lipoprotein receptor knockout mice PMID: 22015660
    25. Placental growth factor finely tunes a balanced regulation of the tissue inhibitor of metalloproteinases-3/tumor necrosis factor-alpha-converting enzyme axis and the consequent TNF-alpha activation in response to transverse aortic constriction. PMID: 21900081
    26. TIMP3 was identified as a potential miR-206 target by TargetScan prediction analysis; further, in cultured cardiac fibroblasts, miR-206 gain- and loss-of-function studies and luciferase reporter assays showed that TIMP3 is a direct target of miR-206. PMID: 21731608
    27. deficiency in TIMP-3 increases cardiac rupture post-myocardial infarction PMID: 21243368
    28. Timp3, an endogenous regulator of metalloproteinases, stimulates hematopoietic stem cells proliferation by recruiting quiescent hematopoietic stem cells into the cell cycle. PMID: 20798233
    29. demonstrated that TNF signaling promoted hepatotoxicity, while excessive TNF receptor 1 (TNFR1) shedding in Timp3-/- mice was protective. PMID: 20628198
    30. TIMP3 functions to promote the resolution of inflammation in the lung. PMID: 20008147
    31. We have identified novel mechanisms, governed by the TACE-Timp3 interaction, involved in the determination of insulin resistance and liver steatosis during overfeeding in mice. PMID: 19877183
    32. Overexpression of TIMP-3 can inhibit angiogenesis and associated tumor growth, and that the antiangiogenic effects of TIMP-3 appear to be mediated through the inhibition of functional capillary morphogenesis. PMID: 12384438
    33. The gene expression and protein distribution of Timp-3 was analyzed during molar development. PMID: 12950084
    34. TIMP-3 deficiency disrupts matrix homeostasis and the balance of inflammatory mediators, eliciting the transition to cardiac dilation and dysfunction. PMID: 15262835
    35. Here we show that deletion of the mouse gene Timp3 resulted in an increase in TNF-alpha converting enzyme activity, constitutive release of TNF and activation of TNF signaling in the liver. PMID: 15322543
    36. Timp3 has a role in diabetes and vascular inflammation through the insulin receptor and TNF-alpha PMID: 16294222
    37. loss of TIMP3 impacts innate immunity by dysregulating cleavage of TNF and its receptors PMID: 16393953
    38. TIMP-3 provides an inherent regulation over the kinetics of pro-MMP-2 processing, serving at a level distinct from that of TIMP-2 and TIMP-4 PMID: 16469749
    39. TIMP-3 regulates MMP-2 activation to limit tumor cell extravasation and subsequent colonization of the lung, without augmenting inflammatory cell response. PMID: 16702949
    40. Timp-3 plays an important role in the maintenance of renal macrostructure after unilateral ureteral obstruction PMID: 16778336
    41. TIMP3 can indirectly regulate epithelial cell proliferation via MMP inhibitory activity. While others have demonstrated that TIMP3 controls proliferation in vitro, this is the first evidence of TIMP3 regulating proliferation in vivo. PMID: 16890932
    42. TIMP3 has a role in the pericyte-induced stabilization of newly formed vascular networks that are predisposed to undergo regression and reveal specific molecular targets of the inhibitors regulating these events. PMID: 17030988
    43. Physiologically, Timp3(-/-) mammary glands displayed accelerated mammary ductal elongation during pubertal morphogenesis. PMID: 17327279
    44. Results indicate that TIMP-3 deficiency results in mild cartilage degradation similar to changes seen in patients with osteoarthritis. PMID: 17328064
    45. Laparotomy induced a downregulation of TIMP-3 and an upregulation of PI3-kinase, which increased cancer cell growth, invasion and cell cycle activity in comparison to laparoscopy. PMID: 17342361
    46. The alveolar macrophage is essential for the TIMP-3 null sepsis-induced compliance alterations. PMID: 17586692
    47. Timp-3 plays an important role in L-NAME-induced hypertension and myocardial vascular remodeling. PMID: 17664861
    48. Role of DPPI and TIMP-3 in development of pulmonary fibrosis in early inflammation following bleomycin instillation. PMID: 17673693
    49. TIMP-3 deficiency accelerated maladaptive cardiac remodeling after a myocardial infarction by promoting matrix degradation and inflammatory cytokine expression. PMID: 17945252
    50. TIMP-3 facilitates Fas-mediated neuronal cell death following oxigen-glucose deprivation and plays a pro-apoptotic role in mild cerebral ischemia PMID: 17962815

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  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 蛋白家族：
    Protease inhibitor I35 (TIMP) family
  • 组织特异性：
    Highest levels are found in kidney, lung and brain followed by ovary and uterus. Low levels are found in bone.
  • 数据库链接：

    KEGG: mmu:21859

    STRING: 10090.ENSMUSP00000020234

    UniGene: Mm.4871