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  4. Mouse tissue inhibitors of metalloproteinase 2,TIMP-2 ELISA kit

Mouse tissue inhibitors of metalloproteinase 2,TIMP-2 ELISA kit

  • 中文名称：
    小鼠基质金属蛋白酶抑制因子2(TIMP-2)酶联免疫试剂盒
  • 货号：
    CSB-E07387m
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    CUSABIO's mouse TIMP2 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse TIMP2 in serum, plasma, cell culture supernates, or tissue homogenates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of TIMP2 in samples. The color intensity is measured at 450 nm via a microplate reader.

    TIMP2 is a unique inhibitor among the TIMP family members, because it not only correlates with matrix remodeling and angiogenesis suppressing but is also involved in the process of tumor growth, inflammation, and other diseases. TIMP2 mediates anti-angiogenic effects by inhibiting endothelial cell migration and invasion through α3β1 integrin. It has been reported to have erythroid-potentiating activity, stimulate quiescent cell proliferation, function as a metanephric mesenchymal growth factor, and inhibit FGF-stimulated endothelial cell growth.

  • 别名：
    Timp2 ELISA Kit; Timp-2 ELISA Kit; Metalloproteinase inhibitor 2 ELISA Kit; Tissue inhibitor of metalloproteinases 2 ELISA Kit; TIMP-2 ELISA Kit
  • 缩写：
    TIMP2
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    125 pg/mL-8000 pg/mL
  • 灵敏度：
    31.25 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse TIMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:20 Average % 106  
    Range % 101-113  
    1:40 Average % 84  
    Range % 80-88  
    1:80 Average % 95  
    Range % 90-100  
    1:160 Average % 98  
    Range % 94-105  
  • 回收率：
    The recovery of mouse TIMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 91 85-96  
    EDTA plasma (n=4) 88 84-93  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    8000 2.218 2.351 2.285 2.110  
    4000 1.674 1.607 1.641 1.466  
    2000 1.241 1.194 1.218 1.043  
    1000 0.802 0.853 0.828 0.653  
    500 0.576 0.612 0.594 0.419  
    250 0.349 0.340 0.345 0.170  
    125 0.261 0.267 0.264 0.089  
    0 0.177 0.172 0.175    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Protease inhibitor I35 (TIMP) family
  • 组织特异性：
    Detected in testis, retina, hippocampus and cerebral cortex.
  • 数据库链接：