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  4. Mouse soluble myosin heavy chain 1,sMHC-1 ELISA Kit

Mouse soluble myosin heavy chain 1,sMHC-1 ELISA Kit

  • 中文名称：
    小鼠可溶性肌球蛋白重链1(sMHC-1)酶联免疫试剂盒
  • 货号：
    CSB-E11155m
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse MYH1 ELISA Kit was designed for the quantitative measurement of Mouse MYH1 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

  • 别名：
    Myh1 ELISA Kit; Myosin-1 ELISA Kit; Myosin heavy chain 1 ELISA Kit; Myosin heavy chain 2x ELISA Kit; MyHC-2x ELISA Kit; Myosin heavy chain ELISA Kit; skeletal muscle ELISA Kit; adult 1 ELISA Kit
  • 缩写：
    MYH1
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays):CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse sMHC-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 92    
    Range % 85-99    
    1:2 Average % 102    
    Range % 97-106    
    1:4 Average % 85    
    Range % 81-90    
    1:8 Average % 104    
    Range % 100-108    
  • 回收率：
    The recovery of mouse sMHC-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 89 85-96    
    EDTA plasma (n=4) 87 92-93    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    1000 2.009 2.061 2.035 1.862    
    500 1.424 1.402 1.413 1.240    
    250 0.980 0.951 0.966 0.793    
    125 0.577 0.589 0.583 0.410    
    62.5 0.380 0.398 0.389 0.216    
    31.2 0.271 0.268 0.270 0.097    
    15.6 0.223 0.215 0.219 0.046    
    0 0.176 0.170 0.173 　    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Muscle contraction.
  • 基因功能参考文献：
    1. Data suggest that single muscle immobilization induces a shift of myosin heavy chain (MHC) isoforms composition toward a faster contractile phenotype and decreases the polymorphic profile of single fibres, and that activation of p38 and JNK could be a potential mechanism involved in these contractile phenotype modifications during muscle immobilization. PMID: 27383612
    2. p38alpha/beta MAPKs-mediated coactivator recruitment at a proximal MEF-2 site is important for MyHCIId/x gene regulation in skeletal muscle. PMID: 17210568
    3. The maintenance of the relative expression of slow MHC-1 protein is independent of neuromuscular activity in mice. PMID: 18945940
  • 亚细胞定位：
    Cytoplasm, myofibril. Note=Thick filaments of the myofibrils.
  • 蛋白家族：
    TRAFAC class myosin-kinesin ATPase superfamily, Myosin family
  • 数据库链接：