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  4. Mouse phosphoenolpyruvate carboxykinase,PCK ELISA Kit

Mouse phosphoenolpyruvate carboxykinase,PCK ELISA Kit

  • 中文名称：
    小鼠磷酸烯醇式丙酮酸羧激酶(PCK)酶联免疫试剂盒
  • 货号：
    CSB-E13613m
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse PCK1 ELISA Kit was designed for the quantitative measurement of Mouse PCK1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 pg/mL-100 pg/mL and the sensitivity is 0.39 pg/mL.

  • 别名：
    Pck1 ELISA Kit; Pepck ELISA Kit; Phosphoenolpyruvate carboxykinase ELISA Kit; cytosolic [GTP] ELISA Kit; PEPCK-C ELISA Kit; EC 4.1.1.32 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    1.56 pg/mL-100 pg/mL
  • 灵敏度：
    0.39 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse PCK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:100 Average % 96    
    Range % 93-99    
    1:200 Average % 88    
    Range % 84-93    
    1:400 Average % 101    
    Range % 97-106    
    1:800 Average % 85    
    Range % 80-91    
  • 回收率：
    The recovery of mouse PCK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 103 100-105    
    EDTA plasma (n=4) 89 85-94    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    100 2.036 2.101 2.069 1.875    
    50 1.631 1.602 1.617 1.423    
    25 1.108 1.201 1.155 0.961    
    12.5 0.789 0.760 0.775 0.581    
    6.25 0.485 0.491 0.488 0.294    
    3.12 0.345 0.339 0.342 0.148    
    1.56 0.275 0.281 0.278 0.084    
    0 0.191 0.196 0.194 　    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Cytosolic phosphoenolpyruvate carboxykinase that catalyzes the reversible decarboxylation and phosphorylation of oxaloacetate (OAA) and acts as the rate-limiting enzyme in gluconeogenesis. Regulates cataplerosis and anaplerosis, the processes that control the levels of metabolic intermediates in the citric acid cycle. At low glucose levels, it catalyzes the cataplerotic conversion of oxaloacetate to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. At high glucose levels, it catalyzes the anaplerotic conversion of phosphoenolpyruvate to oxaloacetate. Acts as a regulator of formation and maintenance of memory CD8(+) T-cells: up-regulated in these cells, where it generates phosphoenolpyruvate, via gluconeogenesis. The resultant phosphoenolpyruvate flows to glycogen and pentose phosphate pathway, which is essential for memory CD8(+) T-cells homeostasis. In addition to the phosphoenolpyruvate carboxykinase activity, also acts as a protein kinase when phosphorylated at Ser-90: phosphorylation at Ser-90 by AKT1 reduces the binding affinity to oxaloacetate and promotes an atypical serine protein kinase activity using GTP as donor. The protein kinase activity regulates lipogenesis: upon phosphorylation at Ser-90, translocates to the endoplasmic reticulum and catalyzes phosphorylation of INSIG proteins (INSIG1 and INSIG2), thereby disrupting the interaction between INSIG proteins and SCAP and promoting nuclear translocation of SREBP proteins (SREBF1/SREBP1 or SREBF2/SREBP2) and subsequent transcription of downstream lipogenesis-related genes.
  • 基因功能参考文献：
    1. Data (including data from studies in knockout mice) suggest that Pck1 in liver plays role in glucose homeostasis; here, Pck1 deficiency (in knockout mice) can be successfully treated by re-expression of Pck1 in liver, thus partially rescuing pups from fatal neonatal hypoglycemia. Pck1 appears to play roles in metabolism in lung and skeletal muscle. PMID: 27785616
    2. Silencing PCK1 or inhibiting its enzymatic activity slows the growth of tumor-repopulating cells in vitro and impedes tumorigenesis in vivo. PMID: 25712344
    3. PEPCK-M expression partially rescued defects in lipid metabolism, gluconeogenesis and TCA cycle function impaired by PEPCK-C deletion, while approximately 10% re-expression of PEPCK-C normalized most parameters. PMID: 23466304
    4. FAT/CD36 regulates PEPCK in adipose tissue and that this could be secondary to reductions in lipolysis. PMID: 23302781
    5. PCB 126 blunted the forskolin-stimulated increase in phosphoenolpyruvate carboxykinase (PEPCK) mRNA levels. PMID: 22615911
    6. there are multiple cytokine pathways by which inflammation inhibits PEPCK expression in adipose tissue which could contribute to the increased mobilization of fatty acids during inflammation PMID: 21450790
    7. Glucagon can also regulate Pck1 in the intestine, but not in the kidneys. PMID: 22013018
    8. Pck1 plays an important role in both the mammary gland adipocytes and epithelial cells during lactation PMID: 21504969
    9. Results suggest that HDAC3 mediates NF-kappaB activity to repress Pepck transcription. PMID: 21406501
    10. Results show the effect of miR-29a in counteracting insulin action on PEPCK gene expression by primarily targeting PI3K and abrogating downstream insulin signaling in HepG2 cells. PMID: 20943204
    11. PPARgamma binding site in the promoter of Pck1 is essential for maintenance of lipid metabolism and glucose homeostasis and disease prevention. PMID: 20124556
    12. hyperhomocysteinemia may be considered as a risk factor that contributes to the development of insulin resistance with respect to elevated glucose output and upregulation of PEPCK, probably via the PKA pathway PMID: 20011977
    13. Data show that gamma-linoleic acid positively modulates TR4 activity to promote the expression of downstream genes apolipoprotein E (ApoE) and phosphoenolpyruvate carboxykinase. PMID: 19800043
    14. ATF3 bound to the ATF/cAMP-responsvie element site derived from the promoter of the gene encoding the gluconeogenic enzyme phosphoenolpyruvate carboxykinase PMID: 11916968
    15. The binding of nuclear receptors to PEPCK RARE1, RARE2 and RARE3 indicates a complex pattern of retinoid receptor and orphan nuclear receptor binding. PMID: 12354671
    16. Liver-specific PEPCK knockout mice are able to maintain normal blood sugar while fasting by increasing gluconeogenesis and by glucose metabolism PMID: 12829628
    17. Reduction in RNA Pol II association is indicative of interruption in direct interactions of RNA Pol II with PEPCK promoter, with general transcription factors and/or with coregulator molecules contributeing to activation of PEPCK gene. PMID: 14652357
    18. PEPCK has a role in tricarboxylic acid cycle activity in mouse liver PMID: 15347677
    19. regulation of glyceroneogenesis and of PEPCK-C gene expression and activity by FAs, retinoic acids, glucocorticoids and the hypolipidemic class of drugs, thiazolidinediones. PMID: 15733733
    20. MKP-3 activates PEPCK gene transcription and increases gluconeogenesis PMID: 16126724
    21. ERRalpha acts as a transcriptional repressor of the PEPCK gene PMID: 16267049
    22. Nuclear receptors bind to retinoic acid response elements (RAREs) in the PEPCK promoter in liver. PMID: 16317119
    23. HNF-4alpha, RXR alpha, RAR alpha, PPAR alpha & chicken ovalbumin upstream promoter transcription factor II bind to the downstream retinoic acid response unit RARE1/RARE2, & PPARalpha & RXRalpha bind to the upstream RARE3 of the PEPCK gene. PMID: 16713227
    24. growth hormone corrects PEPCK expression in aging livers PMID: 17107955
    25. PEPCK expression is coordinate with hepatic energy metabolism to control gluconeogenesis. PMID: 17403375
    26. IFN-gamma exerts a tissue-specific action in rodents and humans, having glyceroneogenesis and the PEPCK-C gene as selective targets to intensify fatty acid release from adipocytes PMID: 17495004
    27. PEPCK-C(mus) mice had an extended life span relative to control animals PMID: 17716967
    28. Data show that knocking down hepatic PEPCK-C resulted in improved glycemia and insulinemia, lower triglyceride, and higher total and HDL cholesterol, and improved insulin signaling in the liver, muscle, and adipose tissue. PMID: 18443203
    29. Reduced insulin sensitivity in fat-fed H-AR(-/y) mice was associated with decreased phosphoinositide-3 kinase activity and increased phosphenolpyruvate carboxykinase expression and correlated with increased protein-tyrosine phosphatase 1B expression. PMID: 18449947
    30. The effects of several variants of retinoic acid on the expression and activity of PEPCK in human, mouse, and rat adipose tissue are reported. PMID: 18492826
    31. Reduced CREB phosphorylation (Ser-129) associated with inactivation of GSK3beta by Ser-9 phosphorylation may be the major mechanism underlying PEPCK-C gene suppression by AMPK-activating agents such as biguanide PMID: 18801732
    32. In vivo phosphoenolpyruvate carboxykinase promoter mapping identifies disrupted hormonal synergism as a target of inflammation during sepsis in mice. PMID: 19821526

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  • 亚细胞定位：
    Cytoplasm, cytosol. Endoplasmic reticulum.
  • 蛋白家族：
    Phosphoenolpyruvate carboxykinase [GTP] family
  • 数据库链接：