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  4. Mouse periostin/osteoblast specific factor 2 (POSTN) ELISA kit

Mouse periostin/osteoblast specific factor 2 (POSTN) ELISA kit

  • 中文名称：
    小鼠骨膜蛋白/成骨细胞特异性因子2(POSTN)酶联免疫试剂盒
  • 货号：
    CSB-EL018381MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：
       POSTN（Periostin）是一种细胞外基质蛋白质，参与了骨骼、心脏和肺部的发育和修复过程。此外，它还在某些癌症的发展中起到了作用。近年来的研究表明，POSTN还可能在哮喘和过敏性疾病中扮演重要角色。人体内POSTN的含量较低，但在某些疾病中，如骨质增生等，POSTN的含量会增加。
       j9九游会登录入口首页生物所提供的Mouse periostin/osteoblast specific factor 2 (POSTN) ELISA kit属于ELISA检测试剂盒，采用双抗夹心法定量检测鼠 血清、血浆、组织匀浆样本中的POSTN，其灵敏度为0.039 ng/mL，检测范围为1.56 ng/mL-100 ng/mL。
     
  • 别名：
    Postn ELISA Kit; Osf2 ELISA Kit; Periostin ELISA Kit; PN ELISA Kit; Osteoblast-specific factor 2 ELISA Kit; OSF-2 ELISA Kit
  • 缩写：
    POSTN
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    1.56 ng/mL-100 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse POSTN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1000 Average % 103    
    Range % 94-107    
    1:2000 Average % 96    
    Range % 85-100    
    1:4000 Average % 91    
    Range % 85-95    
    1:8000 Average % 91    
    Range % 82-94    
  • 回收率：
    The recovery of mouse POSTN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 92 84-96    
    EDTA plasma (n=4) 90 83-95    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average Corrected    
    10 2.511 2.435 2.473 2.299    
    5 2.101 2.075 2.088 1.914    
    2.5 1.452 1.499 1.476 1.302    
    1.25 0.925 0.938 0.932 0.758    
    0.625 0.549 0.573 0.561 0.387    
    0.312 0.402 0.414 0.408 0.234    
    0.156 0.284 0.293 0.289 0.115    
    0 0.172 0.176 0.174 　    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 最新研究进展：
    POSTN是一种细胞外基质蛋白，最近的研究表明它在人体内的生理过程中起到重要的作用。POSTN在骨骼肌肌肉再生和维持中有重要作用，同时也参与了心血管、肺部和神经系统的生理过程，还有可能参与肿瘤的发生和发展。未来的研究将进一步揭示POSTN在生理和病理状态下的作用机制，为开发新的治疗策略提供理论依据。
  • 功能：
    Induces cell attachment and spreading and plays a role in cell adhesion. Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX.
  • 基因功能参考文献：
    1. Inhibition of periostin could be important for suppressing capsule formation on silicone implants after in vivo implantation. PMID: 29854742
    2. Unaffected by Postn, Vav-Itgav(-/-) haematopoietic stem cell proliferate faster in vitro, illustrating the importance of Postn-Itgav interaction. PMID: 27905395
    3. mesenchymal source, specific to cardiovascular tissues, roles in cardiovascular development and disease [review] PMID: 29101484
    4. Results indicate that periostin is a key player in the development of bleomycin-induced fibrosis and consequent enhancement of tumor cell colonization in the lung. PMID: 29498146
    5. Periostin gene deletion impairs periosteal cells functions and fracture consolidation. PMID: 29472541
    6. these data indicate that CatK not only plays a major role in bone remodeling but also modulates modeling-based cortical bone formation by degrading periostin and thereby moderating Wnt-beta-catenin signaling. PMID: 28322464
    7. Ablation of periostin suppresses post-infarction myocardial regeneration by inhibiting the PI3K/GSK3beta/cyclin D1 signalling pathway, indicating that periostin is essential for myocardial regeneration. PMID: 28453729
    8. this study shows that periostin regulates fibrocyte function to promote myofibroblast differentiation and lung fibrosis PMID: 27435108
    9. Genetic or pharmaco-genetic inhibition of periostin in animal models of renal disease was efficient in arresting the progression of the kidney pathology. (Review) PMID: 28884334
    10. TGFBI and periostin act similarly in the heart in affecting fibrosis and disease responsiveness. PMID: 28750100
    11. Results suggest that stromal cell periostin (POSTN) supports both normal hematopoietic progenitor cells (HPCs) and leukemia-initiating cells in vitro. PMID: 27596966
    12. the expressions of the periostin splice variants were increased in ischemic retinas. PMID: 27744020
    13. POSTN regulates the scar formation after spinal cord injuries. PMID: 28082119
    14. periostin in the extracellular matrix architecture prevents pulmonary leakage of blood fluid, thus increasing the survival rate in mice with acute lung injury PMID: 27270966
    15. These results indicated that tensile stress load increased the expression levels of PN in mouse peridontal ligaments and human periodontal ligament cells. PMID: 28260019
    16. POSTN promotes the pulmonary accumulation of myeloid-derived suppressor cells (MDSCs) during the early stage of breast tumour metastasis. Postn deletion decreases neutrophil and monocytic cell populations in the bone marrow of mice and suppresses the accumulation of MDSCs to premetastatic sites. PMID: 27193093
    17. Fibrillin-2 and periostin are target genes in Osterix-mediated osteoblast differentiation. PMID: 27751812
    18. Periostin mediates intestinal inflammation through the activation of NF-kappaB signaling, which suggests that periostin is a potential therapeutic target for inflammatory bowel disease. PMID: 26890265
    19. Thus, these results demonstrate a subcellular role of periostin in promotion of fibronectin secretion from the ER. PMID: 26820539
    20. These data demonstrate that the expression of periostin is stimulated by ErbB ligand NRG1 and influences the migration of Schwann cell precursors. PMID: 26187852
    21. Our data suggest that POSTN is a crucial factor for proper exocrine lineage-specific regeneration after severe acute pancreatitis. PMID: 26632158
    22. Periostin functions as a regulator of atherosclerotic lesion formation and progression. PMID: 26564821
    23. HMGB1 expression in the periodontal ligament is differentially regulated during orthodontic tooth movement in wild type versus periostin-deficient mice. PMID: 25948513
    24. POSTN was expressed in the region surrounding melanoma cell nests in metastatic lesions of both wounded mice and the patient. PMID: 26083413
    25. POSTN significantly enhances neural stem cell proliferation and differentiation after hypoxia-ischemia. PMID: 25894199
    26. loss of Postn in an ErbB2/Neu/HER2 overexpression model results in apocrine-like tumors that activate an AR-dependent pathway. PMID: 25592291
    27. Periostin appears to be a mediator for epidermal hyperplasia that is common to AD and psoriasis. PMID: 25572557
    28. Deficiency in periostin abrogated the development of liver fibrosis in mice. PMID: 25541330
    29. key pathologic factors responsible for bone and periodontal ligament damage in periostin-null mice PMID: 25757567
    30. These results further indicate that periostin is an essential mediator of cortical bone response to mechanical forces (loading and unloading). PMID: 25445447
    31. This study found that the secreted protein periostin (Postn) is highly induced after partial pancreatectomy in regenerating areas containing mesenchymal stroma and tubular complexes. PMID: 25485969
    32. Periostin might be associated with pathophysiology in post-ischemic inflammation and neurogenesis. PMID: 24646642
    33. Overexpression of periostin resulted in reduced expression of peroxisome proliferator-activated receptor alpha (PPARalpha), a master regulator of fatty acid oxidation, and activation of the JNK signaling pathway. PMID: 25003192
    34. In mice, periostin is required for maximal airways hyperresponsiveness and inflammation after house dust mite sensitization and challenge. PMID: 24996263
    35. periostin deficiency may not only cause periodontal ligament collagen fibril disorganization, but could also affect the distribution of other major extracellular matrix proteins such as fibronectin and tenascin C. PMID: 24352874
    36. periostin plays an essential role in the function of collagenolytic enzymes like cathepsin K, MMP1 and MMP2 in the compressed PDL after orthodontic force application. PMID: 24202437
    37. adversely modifies the progression of renal cystic disease by promoting cyst epithelial cell proliferation, cyst enlargement, and interstitial fibrosis PMID: 24284511
    38. Periostin-induced epithelial migration and proliferation requires mTOR signaling. PMID: 24349533
    39. Periostin and a paracrine loop composed of IL-1alpha and IL-6 regulates keratinocyte proliferation and differentiation in the epithelial-mesenchymal interactions, and periostin tunes the magnitude of keratinocyte proliferation and differentiation. PMID: 24352037
    40. Periostin/beta-INTEGRINs/focal adhesion kinase/PI3K/ERK signals promote hyaluronan synthase-2 activation, matrix remodeling, and valve progenitor cell survival/differentiation. PMID: 24469446
    41. the level of periostin expression could influence the propensity to fatigue fractures. PMID: 24167618
    42. Periostin downregulation is an early marker of inhibited neonatal murine lung alveolar septation. PMID: 23723163
    43. Metabolic activity of CYP1B1 contributes to oxidative homeostasis and ultrastructural organization and function of trabecular meshwork tissue through modulation of Postn expression. PMID: 23979599
    44. Clear cell renal cell carcinoma induces fibroblast-mediated accumulation of stromal periostin, enhancing tumor cell attachment and fibroblast proliferation. PMID: 23896380
    45. periostin exacerbates the pathogenesis of AD through TSLP production from keratinocytes. PMID: 22918211
    46. Periostin plays an essential role in the pathogenesis of Bleomycin-induced scleroderma in mice. PMID: 22911870
    47. These results suggest that periostin, which is secreted by the intraocular macrophages, enhances the hyaloidvascular system regression by intensifying the adhesion of macrophages to hyaloid vessels. PMID: 22930727
    48. Periostin regulates beta catenin signaling and parathyroid hormone anabolic effects in bone. PMID: 22927401
    49. Periostin overexpression is associated with kidney injury. PMID: 22167593
    50. periostin sets up a vicious circle that links Th2-type immune responses to keratinocyte activation and plays a critical role in the amplification and chronicity of allergic skin inflammation. PMID: 22684102

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  • 亚细胞定位：
    Golgi apparatus. Secreted. Secreted, extracellular space, extracellular matrix.
  • 组织特异性：
    Preferentially expressed in periosteum and periodontal ligament. Also expressed in the developing and adult heart.
  • 数据库链接：