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Mouse neuregulin-4 (NRG4) ELISA kit

  • 中文名称：
    小鼠神经调节蛋白4(NRG4)酶联免疫试剂盒
  • 货号：
    CSB-EL016080MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse NRG4 ELISA Kit was designed for the quantitative measurement of Mouse NRG4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 ng/mL-200 ng/mL and the sensitivity is 0.78 ng/mL.

  • 别名：
    Nrg4 ELISA Kit; Pro-neuregulin-4 ELISA Kit; membrane-bound isoform ELISA Kit; Pro-NRG4) [Cleaved into: Neuregulin-4 ELISA Kit; NRG-4)] ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    3.12 ng/mL-200 ng/mL
  • 灵敏度：
    0.78 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse NRG4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 97
    Range % 92-103
    1:2 Average % 91
    Range % 84-96
    1:4 Average % 90
    Range % 87-95
    1:8 Average % 100
    Range % 95-105
  • 回收率：
    The recovery of mouse NRG4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 90 87-93
    EDTA plasma (n=4) 94 90-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    200 2.549 2.763 2.656 2.556
    100 2.135 2.131 2.133 2.033
    50 1.587 1.622 1.605 1.505
    25 1.014 1.033 1.024 0.924
    12.5 0.736 0.787 0.762 0.662
    6.25 0.468 0.436 0.452 0.352
    3.12 0.214 0.231 0.223 0.123
    0 0.101 0.098 0.100 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Low affinity ligand for the ERBB4 tyrosine kinase receptor. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. Does not bind to the ERBB1, ERBB2 and ERBB3 receptors.
  • 基因功能参考文献：
    1. The found of this study showed that NRG4 is a major novel regulator of dendritic arborisation in the developing cerebral cortex and suggest that it exerts its effects by an autocrine/paracrine mechanism. PMID: 29317193
    2. Conditional knockout of Nrg4 in brown adipocytes caused blood vessel reduction in brown but not in white adipose tissue , and was sufficient to induce obese phenotype. PMID: 29902456
    3. Nrg4 exerts beneficial effects on energy balance and glucose and lipid metabolism to ameliorate obesity-associated metabolic disorders. PMID: 28752050
    4. overexpression of the Nrg4 gene by hydrodynamic gene delivery prevents high fat diet-induced weight gain and fatty liver, alleviates obesity-induced chronic inflammation and insulin resistance PMID: 27184920
    5. Pharmacological inhibition of PI3K/Akt signaling reversed the anti-apoptotic effects of NRG4, confirming the role of this cascade in NRG4-induced cell survival PMID: 23033483

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  • 亚细胞定位：
    [Pro-neuregulin-4, membrane-bound isoform]: Cell membrane; Single-pass type I membrane protein.; [Neuregulin-4]: Secreted.
  • 蛋白家族：
    Neuregulin family
  • 组织特异性：
    Highly expressed in pancreas; weakly expressed in muscle.
  • 数据库链接：