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  4. Mouse mucin-5 subtype B,MUC5B ELISA Kit

Mouse mucin-5 subtype B,MUC5B ELISA Kit

  • 中文名称：
    小鼠粘蛋白/粘液素5B(MUC5B)酶联免疫试剂盒
  • 货号：
    CSB-E15067m
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    The mouse MUC5B Elisa kit is suitable for the quantitative measurement of mouse MUC5B in serum, plasma, cell culture supernates, or tissue homogenates. This assay employs the sandwich enzyme immunoassay technique and enzyme-substrate chromogenic reaction. The color develops positively to the amount of MUC5B in samples. The color development is stopped and the intensity of the color is measured. This kit displays many advantages, including high sensitivity, strong specificity, good linearity, high precision and recovery, as well as lot-to-lot consistency.

    MUC5B is the major gel-forming mucin in the lung that is secreted from proximal submucosal glands and distal airway secretory cells. It plays a key role in mucociliary clearance (MCC) and host defense. MUC5B has a critical protective function in the normal lung, salivary glands, esophagus, and gallbladder, and has been reported to be abnormally expressed in breast cancer. Previous studies have shown that MUC5B is important for tumor progression. Dysregulation of MUC5B expression has also been reported in airway diseases.

  • 缩写：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    1.25 ng/mL-80 ng/mL
  • 灵敏度：
    0.31 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse MUC5B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %87
    Range %81-94
    1:2Average %96
    Range %91-102
    1:4Average %99
    Range %93-105
    1:8Average %92
    Range %86-98
  • 回收率：
    The recovery of mouse MUC5B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9387-96
    EDTA plasma (n=4)9890-105
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    802.236 2.141 2.189 2.098
    401.879 1.801 1.840 1.749
    201.461 1.369 1.415 1.324
    100.853 0.793 0.823 0.732
    50.512 0.496 0.504 0.413
    2.50.307 0.301 0.304 0.213
    1.250.195 0.186 0.191 0.100
    00.093 0.088 0.091 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

平均分:
5.0分 - 2 个评价

样品类型：组织匀浆液 肺组织匀浆上清

样品信息：小鼠

稀释比：没有稀释

产品评价： 我通过使用CSB-E15067m检测了PR8病毒感染的小鼠肺组织匀浆上清，操作简单方便易上手，灵敏度非常高，检测结果十分精准

By 邓老师

样品类型：组织匀浆液 肺组织匀浆上清

样品信息：小鼠

稀释比：没有稀释

产品评价： 我通过使用CSB-E15067m检测了PR8病毒感染的小鼠肺组织匀浆上清，操作简单方便易上手，灵敏度非常高，检测结果十分精准

By 邓老师