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  4. Mouse monocyte chemotactic protein 2,MCP-2 ELISA kit

Mouse monocyte chemotactic protein 2,MCP-2 ELISA kit

  • 中文名称：
    小鼠单核细胞趋化蛋白2(MCP-2/CCL8)酶联免疫试剂盒
  • 货号：
    CSB-E07428m
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse CCL8 ELISA Kit was designed for the quantitative measurement of Mouse CCL8 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL.

  • 别名：
    Ccl8 ELISA Kit; Mcp2 ELISA Kit; Scya8C-C motif chemokine 8 ELISA Kit; Monocyte chemoattractant protein 2 ELISA Kit; Monocyte chemotactic protein 2 ELISA Kit; MCP-2 ELISA Kit; Small-inducible cytokine A8 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    6.25 pg/mL-400 pg/mL
  • 灵敏度：
    1.56 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse MCP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %87
    Range %85-88
    1:2Average %102
    Range %98-106
    1:4Average %98
    Range %96-101
    1:8Average %92
    Range %89-95
  • 回收率：
    The recovery of mouse MCP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8884-92
    EDTA plasma (n=4)9592-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    4002.955 2.781 2.868 2.720
    2002.138 2.156 2.147 1.999
    1001.445 1.532 1.489 1.341
    500.921 0.903 0.912 0.764
    250.584 0.561 0.573 0.425
    12.50.333 0.324 0.329 0.181
    6.250.241 0.253 0.247 0.099
    00.142 0.153 0.148
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Chemotactic factor that attracts monocytes. This protein can bind heparin.
  • 基因功能参考文献：
    1. Findings exemplify how gradients of chemoattractive factors such as CCL8, drive metastasis and suggest that interference with their operation may provide means for breast cancer management. PMID: 27181207
    2. these data reveal an important role for BLIMP1 in modulating host defenses by suppressing expression of the chemokine CCL8. PMID: 24477914
    3. Results indicate that the induction of MCP-2/CCL8 by mycobacteria is dependent on the activation of TLR2/PI3K/Akt signaling pathway. PMID: 23418602
    4. Vitamin B6 supplementation downregulates Cd8a and Ccl8 mRNA expression, so these candidate genes may play a protective role against colonic aberrant crypt foci development. PMID: 22495988
    5. early and preclinical expression of CCL8 in plasma predicts overall survival of graft-vs.-host disease (GVHD) mice. Together with an involvement of allo-recognition in CCL8 expression, it suggests that CCL8 plays an important role in GVHD pathology. PMID: 21782767
    6. MCP/CCL8 was induced via STAT3 and exhibited anti-tumor metastatic effect PMID: 21624767
    7. promotes atopic dermatitis by recruiting IL-5+ Th2 cells PMID: 21217759
    8. CCL8 proteins were present in the vast majority of tissues investigated. mRNA for these proteins was also expressed in most of these tissues suggesting local production and the ability to respond in situ to inflammatory stimuli. PMID: 20931267
    9. Data show that that the stromal cell-derived chemokines CXCL12 and CCL8 cooperate to attract hematopoietic progenitors with the potential to differentiate into regulatory DCs. PMID: 20624948
    10. CCL8 is a promising specific serum marker for the early and accurate diagnosis of graft-versus-host disease. PMID: 18256320
    11. An elevated plasma CCL8 concentration may be a promising plasma marker for GVHD in mouse models PMID: 19302923

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine beta (chemokine CC) family
  • 数据库链接：