Mouse granzyme B (GZMB) ELISA Kit

Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse. It cleaves after Asp. Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.

1. Tcf1 binding reduced the activity of a Gzmb-associated regulatory element, and this accounted for the reduced Gzmb expression in Tcf1-expressing NK cells. PMID: 28723565
2. Differential proteomics were used to identify granzyme B substrates in three unrelated bacteria: Escherichia coli, Listeria monocytogenes, and Mycobacteria tuberculosis. Granzyme B cleaves a highly conserved set of proteins in all three bacteria, which function in vital biosynthetic and metabolic pathways that are critical for bacterial survival under diverse environmental conditions. PMID: 29107333
3. Released granzyme B induces DNA fragmentation in intraepithelial lymphocytes independently of Perforin PMID: 25750028
4. serglycin plays a critical role in the maturation of dense-core cytotoxic granules in cytotoxic lymphocytes and the trafficking and storage of perforin and granzyme B, whereas granzyme A is unaffected PMID: 26756195
5. these results suggest a perforin-independent, extracellular role for GzmB in the pathogenesis of cardiac fibrosis PMID: 26610869
6. data indicate an involvement of granzyme B in the neuroinflammation mediated by Eomes(+) CD4(+) T cells. PMID: 26436530
7. Data suggest that targeting granzyme B (GzmB) in selected T cell subsets may provide a strategy to control graft-versus-host disease (GVHD). PMID: 26392464
8. We conclude that GzmbCre can be used under some conditions to investigate gene function in mature and activated natural killer cells. PMID: 25923440
9. These findings indicate a significant role for GzmB in extracellular matrix degradation that may have implications in many age-related chronic inflammatory diseases. PMID: 25495009
10. Different natural variants of granzyme B have a profound impact on the immune response to a common and authentic viral pathogen. PMID: 25502180
11. Visualization of granzyme B-expressing CD8 T cells during primary and secondary immune responses to Listeria monocytogenes. PMID: 25367158
12. expedites cytotoxic T-lymphocyte diapedesis via basement membrane remodeling PMID: 25526309
13. CD4(+) natural killer cells potently promote atherosclerosis by granzyme B (and perforin)-dependent apoptosis. PMID: 25398236
14. Granzyme B has a role in atherosclerotic plaque development. PMID: 24205352
15. Data indicate that vascular permeability is reduced in Granzyme B (GZMB)-KO mice after delayed-type hypersensitivity -induced inflammation. PMID: 24791744
16. GzmB is a negative regulator of hematopoietic stem cell function that is induced by stress and chemotherapy in both HSCs and their niches. PMID: 24752302
17. studies established a pivotal role for NK-cell NKG2D and granzyme B in the pathogenesis of house dust mite-allergic lung disease, and identified novel therapeutic targets for the prevention and treatment of asthma. PMID: 24290277
18. The apoptotic cell-in-cell death occurred only in internalized immune killer cells expressing granzyme B PMID: 24113190
19. Granzyme B deficiency exacerbates lung inflammation in mice after acute lung injury. PMID: 23642129
20. Genetic deletion of granzyme B does not confer resistance to the development of spontaneous diabetes in non-obese diabetic mice. PMID: 23663075
21. in the Period 1 gene (Per1(-/-) mice), involved in the negative limb of the molecular clock, display significantly altered rhythms of cytokine (eg, interferon-gamma) and cytolytic factors (eg, perforin and granzyme B) in splenic natural killer (NK) cells. PMID: 23402528
22. granzyme B is dispensable for beta cell destruction in type 1 diabetes, but is required for efficient early activation of cytotxic T lymphocytes. PMID: 22792290
23. GzmB-mediated, activation-induced cell death in wild-type T8 cells was responsible for their reduced graft-vs-tumor activity. GzmB(-/-) T8 cells had enhanced expansion, skewed toward an effector/memory phenotype, & made more IFN-gamma & Fas ligand. PMID: 23264653
24. expression by regulatory t-cells controls lung inflammation during acute viral infection PMID: 22236998
25. There is a role for Granzyme B in age-related skin thinning and frailty. PMID: 21316440
26. Data suggest that an interdependent relationship between parasite burden and CD8(+) T cells dictates the onset of perforin/GzmB-mediated ECM. PMID: 21525386
27. During lymphocytic choriomeningitis virus infection, despite persistent similarities in chromatin of CD8-positive memory cells, continuous transcription does not occur at the granzyme B locus. PMID: 21278341
28. GzmA/B deficiency in filarial infection is associated with a defense-promoting Th2 cytokine and antibody shift, enhanced early inflammatory gene expression, and a trend of reduced alternatively activated macrophage induction. PMID: 21248253
29. The herpes simplex virus type 1 LAT protects neuron cells from granzyme B-induced apoptosis and CD8 T-cell killing. PMID: 21177822
30. miR-223 specifically targets the 3' untranslated region of murine GzmB in vitro, indicating that this miRNA may contribute to control of GzmB translation in resting NK cells. PMID: 20935160
31. Data show that increased activation-induced apoptosis resulted in impaired survival and a decreased clonal burst size of Spi6 KO iNKT cells, which could be corrected by GrB deficiency. PMID: 20543105
32. Surprisingly, adoptive transfer of granzyme B-deficient T(reg) cells prevented GVHD lethality, suppressed serum cytokine production in vivo, and prevented target organ damage. PMID: 19965675
33. Pathogenetically relevant immune reactions in proteolipid protein-overexpressing mice are TCR-dependent and mediated by the classical components of CD8+ T-cell cytotoxicity, perforin, and Gzmb. PMID: 20042681
34. gzmB has a role in caspase-dependent inhibition of mousepox replication PMID: 19838298
35. In all tumor models examined thus far, granzyme B is not necessary for tumor rejection mediated by the perforin pathway in vivo. PMID: 12847210
36. Granzyme-mediated cytotoxicity does not involve the mannose 6-phosphate receptors on target cells PMID: 14985351
37. Granzyme-B is one of the key mechanisms through which CD4+CD25+ regulatory T cells induce cell contact-mediated suppression. PMID: 15699103
38. polymorphonuclear leukocytes from mice and humans lack the 3 cytotoxic effector molecules, granzyme A, granzyme B, and perforin, generally associated with natural killer and cytotoxic T lymphocytes PMID: 15998831
39. suppression of natural killer cell activity induced by whole body hyperthermia could be mediated through the perforin/granzyme pathway PMID: 16236268
40. In this study, we discuss our recent findings on granzyme B-induced cell death and discuss the potential relevance of this pathway to CL-induced death of viral-infected and transformed cells. PMID: 16405654
41. absence of either perforin or granzyme B resulted in cell survival in alloreactive CTL-induced pancreatic beta cells PMID: 16436955
42. GrB plays a critical role in the T cell receptor-induced cell death of Th2 cells PMID: 16901729
43. Hop may be cleaved by GzmB as an "innocent bystander" during the induction of apoptosis, but it may also act to facilitate apoptosis in concert with other GzmB substrates PMID: 17005566
44. Granzyme B expression is augmented in activated T cells from Bcl6-/- knockout mice. PMID: 17125145
45. Varies considerably more in wild populations (2-18 amino acid differences) than between common laboratory strains (12 of 13 were identical). PMID: 17661907
46. This is the first report of a major distal regulatory element in the control of granzyme B transcription. PMID: 18222115
47. For the first time, in vivo data implicate granzyme B expression by regulatory T (Treg) lymphocytes in sustaining long-lived graft survival. PMID: 18802078
48. In vitro protease substrate profiling of the apoptotic serine protease granzyme B was performed resulting in the delineation of more than 800 cleavage sites in 322 human and 282 mouse substrates PMID: 18836177
49. granzyme C is activated with persistent antigenic stimulation, providing nonredundant backup protection for the host when granzyme B fails. PMID: 19414782
50. for high levels of transcription to occur a distinct set of histone modifications needs to be established in addition to histone loss at the proximal promoter of gzmB PMID: 19915065

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Secreted. Cytolytic granule.

Peptidase S1 family, Granzyme subfamily

KEGG: mmu:14939

STRING: 10090.ENSMUSP00000015581

UniGene: Mm.14874