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  4. Mouse glucagon-like peptide-1,GLP-1 ELISA Kit

Mouse glucagon-like peptide-1,GLP-1 ELISA Kit

  • 中文名称：
    小鼠胰高血糖素样肽1(GLP-1)酶联免疫试剂盒
  • 货号：
    CSB-E08118m
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    The Mouse glucagon-like peptide-1 (GLP-1) ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of mouse GLP-1in many biological fluids, including serum, plasma, or tissue homogenates. GLP-1 is a gut-derived peptide secreted from intestinal L-cells following a meal. The action of GLP-1 is through the binding of a specific GLP-1 receptor, which is expressed in various tissues, including pancreatic beta cells, pancreatic ducts, and gastric mucosa. GLP-1 exerts its main effect by enhancing glucose-dependent insulin production. GLP-1 has also been demonstrated to delay gastric emptying, suppress inappropriate post-meal glucagon release, and increase satiety.

    This assay employs the Sandwich-ELISA technique, in which GLP-1 in the samples or standards is sandwiched between pre-coated an anti-mouse GLP-1 and Biotin-labeled GLP-1 antibody. The Ag-Ab-Ag immune complex is labeled with HRP-avidin and then develops a color reaction after the addition of the TMB substrate. The intensity of the color is positively correlated to the amount of GLP-1 in the sample. The kit has a detection range of 62.5 ng/mL-4000 ng/mL, excellent sensitivity, high specificity, good linearity, precision low than 10%, high recovery, good lot-to-lot consistency. Get access to more details from the product instructions.

  • 别名：
    N/A
  • 缩写：
    GLP-1
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates.
  • 检测范围：
    125 pg/ml-8000 pg/ml.
  • 灵敏度：
    31.25 pg/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 线性度：
  • 本试剂盒所含材料：
      • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-mouse GLP-1 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • One vial Biotin-labeled GLP-1 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
      • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
      • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
      • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
      • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
      • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

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  • 本试剂盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

平均分:
5.0分 - 1 个评价

样品类型：血清

样品信息：小鼠

稀释比：其他 1：5

产品评价： 我用 CSB-E08118m检测自发性T2DM小鼠db/db小鼠，稀释比例为1：5，稀释后浓度较低，检测出浓度较低。血清较多的话不稀释最好，实验结果准确！

By 丁老师

问答及客户评论

■ 客户评论
  • Sample type: Serum
    Sample species: Mouse
    Review:Administration of GLP-1, reserpine and polytherapy with GLP-1 and reserpine increases GLP-1 levels in diabetic mice. Note that GLP-1 levels in mice after GLP-1/reserpine polytherapy are significantly higher compared to both diabetic mice and diabetic mice after GLP-1 and reserpine monotherapy.
  • Sample type: Plasma
    Sample species: Mouse
    Review: Plasma levels of GLP-1 by ELISA.
    PMID: 30605678
  • Sample type: Serum, bronchoalveolar lavage fluid, lung tissue
    Sample species: Mouse
    Review: Liraglutide restored LPS-induced decrease of GLP-1 in serum, b reduced LPS-induced increase of GLP-1 in bronchoalveolar lavage fluid (BALF).
    PMID: 30659271
  • Sample type: Serum
    Sample species: Mouse
    Review: Levels of serum GLP-1. Data are presented as mean ± SEM. Scale bar, 50 μm. **p<0.01 and *p<0.05 versus model group, respectively. Normal, normal mice group; Model, T2D mice group; Met, T2D mice group administrated with metformin at 200 mg/kg/day; GXG-H, T2D mice group administrated with 200 mg/kg/day; GXG-L, T2D mice group administrated with 50 mg/kg/day. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.).