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  4. Mouse estrogen,E ELISA Kit

Mouse estrogen,E ELISA Kit

  • 中文名称：
    小鼠雌激素(E)酶联免疫试剂盒
  • 货号：
    CSB-E07280m
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

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  • 产品描述：

    This Mouse estrogen (E) ELISA Kit was designed for the quantitative measurement of Mouse estrogen (E) protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 25 pg/mL-1000 pg/mL and the sensitivity is 25 pg/mL.

  • 别名：
    N/A
  • 缩写：
    E
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma
  • 检测范围：
    25 pg/mL-1000 pg/mL
  • 灵敏度：
    25 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse estrogen in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 95  
    Range % 90-100  
    1:2 Average % 92  
    Range % 86-98  
    1:4 Average % 98  
    Range % 95-105  
    1:8 Average % 90  
    Range % 88-92  
  • 回收率：
    The recovery of mouse estrogen spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 90-98  
    EDTA plasma (n=4) 96 91-100  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    0 2.411 2.397 2.404    
    25 1.485 1.526 1.506    
    75 0.900 0.858 0.879    
    230 0.564 0.577 0.571    
    500 0.386 0.364 0.375    
    1000 0.182 0.183 0.183    
  • 本试剂盒所含材料：
    • A 96-well Assay plate--The 96-well plate has been pre-coated with goat-anti-rabbit estrogen antibody.
    • Standard (6 x 0.5 ml)--Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
    • HRP-conjugated estrogen(1 x 6 ml) --Bind to the anti-mouse estrogen antibody, and HRP catalyzes the TMB to elicit a chromogenic reaction.
    • Anti-mouse estrogen Antibody (1 x 6 ml)--React with both estrogen in the samples/standards and HRP-conjugated estrogen.
    • Wash Buffer (20x concentrate) 1 x 15ml --Wash away unbound or free substances.
    • Substrate A (1 x 7 ml) --Mix with Substrate B and interacts with HRP, eliciting the solution turns blue.
    • Substrate B (1 x 7 ml) --Mix with Substrate A and interacts with HRP, eliciting the solution turns blue.
    • Stop Solution (1 x 7ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells)
    • An Instruction manual

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  • 本试剂盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
    • An incubator that can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

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