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Mouse catestatin ELISA Kit

  • 中文名称：
    小鼠儿茶酚抑素酶联免疫试剂盒
  • 货号：
    CSB-E17357m
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse catestatin ELISA Kit was designed for the quantitative measurement of Mouse catestatin protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 78 pg/mL-5000 pg/mL and the sensitivity is 19.5 pg/mL.

  • 缩写：
    catestatin
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    78 pg/mL-5000 pg/mL
  • 灵敏度：
    19.5 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Biochemicals
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse catestatin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 87-96  
    1:2 Average % 99  
    Range % 95-103  
    1:4 Average % 101  
    Range % 94-106  
    1:8 Average % 95  
    Range % 89-98  
  • 回收率：
    The recovery of mouse catestatin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 91 86-98  
    EDTA plasma (n=4) 99 92-106  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    5000 2.989 3.002 2.996 2.853  
    2500 2.547 2.444 2.496 2.353  
    1250 1.984 1.998 1.991 1.848  
    625 1.258 1.168 1.213 1.070  
    312 0.655 0.643 0.649 0.506  
    156 0.502 0.499 0.501 0.358  
    78 0.306 0.325 0.316 0.173  
    0 0.138 0.147 0.143    
  • 数据处理：
  • 货期：
    3-5 working days

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