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  4. Mouse calcitonin gene related peptide (CGRP) ELISA kit

Mouse calcitonin gene related peptide (CGRP) ELISA kit

  • 中文名称：
    小鼠降钙素基因相关肽(CGRP)酶联免疫试剂盒
  • 货号：
    CSB-EQ027706MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 别名：
    Beta type CGRP、Calcitonin gene-related peptide II、CGRPII、Calcitonin gene-related peptide 2、CALCB
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CGRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 94  
    Range % 86-98  
    1:2 Average % 92  
    Range % 88-97  
    1:4 Average % 90  
    Range % 85-96  
    1:8 Average % 94  
    Range % 88-99  
  • 回收率：
    The recovery of mouse CGRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 93 89-97  
    EDTA plasma (n=4) 96 91-99  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    1000 2.342 2.321 2.332 2.178  
    500 1.772 1.760 1.766 1.612  
    250 1.250 1.241 1.246 1.092  
    125 0.799 0.789 0.794 0.640  
    62.5 0.499 0.487 0.493 0.339  
    31.25 0.364 0.341 0.353 0.199  
    15.6 0.253 0.244 0.249 0.095  
    0 0.156 0.152 0.154 　  
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    CGRP induces vasodilation. It dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Calcitonin family
  • 组织特异性：
    Detected in nerve cells of cerebrum, hippocampus and pons/midbrain in newborns, and only in nerve cells of pons/midbrain in adult.
  • 数据库链接：