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  4. Mouse anti-double stranded DNA antibody(IgM)ELISA Kit

Mouse anti-double stranded DNA antibody(IgM)ELISA Kit

  • 中文名称：
    小鼠抗双链DNA抗体/天然DNA抗体(IgM)酶联免疫试剂盒
  • 货号：
    CSB-E12911m
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse dsDNA Ab (IgM) ELISA Kit was designed for the quantitative measurement of Mouse dsDNA Ab (IgM) protein in serum, plasma. It is a Capture ELISA ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL.

  • 缩写：
    dsDNA Ab (IgM)
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma
  • 检测范围：
    1.56 ng/mL-100 ng/mL
  • 灵敏度：
    0.39 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Capture ELISA
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse anti-double stranded DNA antibody (IgM) (dsDNA Ab (IgM)) in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %95
    Range %85-105
    1:2Average %89
    Range %85-98
    1:4Average %93
    Range %85-97
    1:8Average %95
    Range %89-110
  • 回收率：
    The recovery of mouse anti-double stranded DNA antibody (IgM) (dsDNA Ab (IgM)) spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9483-101
    EDTA plasma (n=4)9787-106
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    1002.501 2.531 2.516 2.377
    502.178 2.203 2.191 2.052
    251.803 1.822 1.813 1.674
    12.51.254 1.268 1.261 1.122
    6.250.731 0.745 0.738 0.599
    3.120.405 0.397 0.401 0.262
    1.560.255 0.249 0.252 0.113
    00.138 0.140 0.139
  • 数据处理：
  • 货期：
    3-5 working days

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