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  4. Mouse alpha-1-microglobulin/bikunin precursor (AMBP) ELISA kit

Mouse alpha-1-microglobulin/bikunin precursor (AMBP) ELISA kit

  • 中文名称：
    小鼠α1微球蛋白/bikunin蛋白前体(AMBP)酶联免疫试剂盒
  • 货号：
    CSB-E09776m
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse AMBP ELISA Kit was designed for the quantitative measurement of Mouse AMBP protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 1.563 ng/mL-100 ng/mL and the sensitivity is 0.649 ng/mL.

  • 别名：
    Ambp ELISA Kit; ItilProtein AMBP [Cleaved into: Alpha-1-microglobulin; Inter-alpha-trypsin inhibitor light chain ELISA Kit; ITI-LC ELISA Kit; Bikunin ELISA Kit; HI-30); Trypstatin] ELISA Kit
  • 缩写：
    AMBP
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    1.563 ng/mL-100 ng/mL
  • 灵敏度：
    0.649 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse AMBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:100 Average % 104    
    Range % 101-107    
    1:200 Average % 96    
    Range % 93-99    
    1:400 Average % 94    
    Range % 90-97    
    1:800 Average % 91    
    Range % 84-95    
  • 回收率：
    The recovery of mouse AMBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 90 87-93    
    EDTA plasma (n=4) 97 92-102    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average      
    100 0.140 0.145 0.143      
    50 0.357 0.366 0.362      
    25 0.569 0.578 0.574      
    12.5 0.865 0.898 0.882      
    6.25 1.544 1.532 1.538      
    3.125 2.083 2.098 2.091      
    1.563 2.469 2.466 2.468      
    0 2.844 2.878 2.861      
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 功能：
    Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization.; Trypstatin is a trypsin inhibitor.
  • 基因功能参考文献：
    1. Loss of bikunin expression is associated with Embryos failing to Initiate Midgut Rotation. PMID: 30174180
    2. In this review, the use of genetically targeted mice selectively lacking UTI provides evidence of a therapeutic option for experimental endotoxin-related inflammatory disorders such as acute lung injury and acute liver injury. PMID: 20367192
    3. expression of the AMBP gene transcript and its two protein products, alpha(1)-microglobulin and bikunin, in embryogenesis PMID: 12204273
    4. UTI inhibited LPS-induced production of TNF-alpha by inhibiting activation of the extracellular signal-regulated protein kinases 1/2-Egr-1 pathway in monocytes, which might at least partly contribute to reduction of hypotension PMID: 15539418
    5. UTI protects against systemic inflammatory response and subsequent organ injury induced by bacterial endotoxin. PMID: 15576631
    6. results indicate that endogenous UTI is protective against acute lung injury induced by bacterial endotoxin, at least partly, via the antioxidative properties PMID: 16273282
    7. increased sensitivity of Bik-/- mice to lung metastasis in vivo is due to a lack of circulating proteins of the inter-alpha inhibitor family, especially bikunin PMID: 16331631
    8. UTI reduces NF-kappaB activation and downregulates the expression of its related mediators, followed by the inhibition of neutrophil aggregation and infiltration in hepatic ischemia reperfusion injury PMID: 19208516
    9. Report impaired liver regeneration with humoral and genetic disturbances in urinary trypsin inhibitor-deficient mice. PMID: 19302180
    10. UTI protects against severe liver injury and subsequent coagulatory disturbance which was mediated, at least partly, through the suppression of TNF-alpha production along with its antiprotease activity. PMID: 19398962

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Calycin superfamily, Lipocalin family
  • 组织特异性：
    Expressed by the liver and secreted in plasma. Alpha-1-microglobulin occurs in many physiological fluids including plasma, urine, and cerebrospinal fluid. Inter-alpha-trypsin inhibitor is present in plasma and urine.
  • 数据库链接：

    KEGG: mmu:11699

    STRING: 10090.ENSMUSP00000030041

    UniGene: Mm.2197