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Mouse acetyl-CoA ELISA Kit

  • 中文名称：
    小鼠乙酰辅酶A酶联免疫试剂盒
  • 货号：
    CSB-E12936m
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse acetyl-CoA ELISA Kit was designed for the quantitative measurement of Mouse acetyl-CoA protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.78 ng/mL-50 ng/mL and the sensitivity is 0.195 ng/mL.

  • 缩写：
    acetyl-CoA
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    0.78 ng/mL-50 ng/mL
  • 灵敏度：
    0.195 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse Acetyl-CoA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:20 Average % 91  
    Range % 87-93  
    1:40 Average % 103  
    Range % 100-105  
    1:80 Average % 93  
    Range % 87-96  
    1:160 Average % 98  
    Range % 93-101  
  • 回收率：
    The recovery of mouse Acetyl-CoA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 89-97  
    EDTA plasma (n=4) 96 92-98  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    50 2.192 2.114 2.153 1.996  
    25 1.632 1.647 1.640 1.483  
    12.5 1.124 1.110 1.117 0.960  
    6.25 0.713 0.688 0.701 0.544  
    3.12 0.404 0.421 0.413 0.256  
    1.56 0.291 0.278 0.285 0.128  
    0.78 0.220 0.212 0.216 0.059  
    0 0.158 0.156 0.157 　  
  • 数据处理：
  • 货期：
    3-5 working days

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