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  4. Mouse Tumor necrosis factor-inducible gene 6 protein(TNFAIP6) ELISA kit

Mouse Tumor necrosis factor-inducible gene 6 protein(TNFAIP6) ELISA kit

  • 中文名称：
    小鼠肿瘤坏死因子诱导基因蛋白6(TNFAIP6)酶联免疫试剂盒
  • 货号：
    CSB-EL023959MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse TNFAIP6 ELISA Kit was designed for the quantitative measurement of Mouse TNFAIP6 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL .

  • 别名：
    Tnfaip6 ELISA kit; Tnfip6 ELISA kit; Tsg6Tumor necrosis factor-inducible gene 6 protein ELISA kit; TNF-stimulated gene 6 protein ELISA kit; TSG-6 ELISA kit; Tumor necrosis factor alpha-induced protein 6 ELISA kit; TNF alpha-induced protein 6 ELISA kit
  • 缩写：
    TNFAIP6
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    62.5 pg/mL-4000 pg/mL
  • 灵敏度：
    15.6 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse TNFAIP6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %92
    Range %88-96
    1:2Average %90
    Range %85-95
    1:4Average %93
    Range %89-97
    1:8Average %97
    Range %93-101
  • 回收率：
    The recovery of mouse TNFAIP6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9086-95
    EDTA plasma (n=4)9490-99
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    40002.596 2.452 2.524 2.334
    20002.136 2.173 2.155 1.965
    10001.739 1.704 1.722 1.532
    5001.238 1.179 1.209 1.019
    2500.707 0.761 0.734 0.544
    1250.428 0.402 0.415 0.225
    62.50.259 0.277 0.268 0.078
    00.193 0.187 0.190 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Possibly involved in cell-cell and cell-matrix interactions during inflammation and tumorigenesis.
  • 基因功能参考文献：
    1. TSG-6 deficiency protects against airway hyperresponsiveness after ozone (in vivo) or short-fragment hyaluronan (sHA) (in vitro and in vivo) exposure. Moreover, TSG-6(-/-) tracheal ring non-responsiveness to sHA was reversed by exogenous TSG-6 addition. PMID: 29122888
    2. Loss of TSG6 expression is associated with Embryos failing to Initiate Midgut Rotation. PMID: 30174180
    3. TSG-6 up-regulation counteracts the progression of atherosclerosis. (Review) PMID: 29401724
    4. our results showed that the increase of oxidative stress, activation of the NLRP3 inflammasome and NF-kappaB signaling in SAP was substantially inhibited following administration of hMSCs or TSG-6, which was dependent on the presence of CD-44 receptors in acinar cells. PMID: 27917949
    5. TSG-6 protects hepatocytes from damage by enhancing autophagy influx and contributes to liver regeneration PMID: 28935975
    6. TSG6 functions by converting macrophages from a proinflammatory to an anti-inflammatory phenotype secondary to suppression of TLR4/NF-kappaB signaling and STAT1 and STAT3 activation. PMID: 27911817
    7. This study provided the first evidence of TSG-6 secreted by mesenchymal stem cells promoting corneal epithelial wound healing in diabetic mice through activating corneal epithelial stem/progenitor cells and accelerating M2 macrophage polarization PMID: 28810264
    8. This study shows that TSG-6 is expressed in the CNS, suggesting a role for TSG-6 in astrocyte activation and tissue repair. PMID: 27435674
    9. Data (including data from studies in knockout mice) suggest that TSG-6 is needed for release of hyaluronan rafts (leukocyte-adhesive rafts) from apical surface of tracheal mucosa into bronchoalveolar lavage fluid as heavy chain hyaluronan complexes. PMID: 26601955
    10. the well characterized hyaluronan-binding site in the TSG-6 Link module is not used for recognition during transfer of HCs onto HA. PMID: 26468290
    11. Thus, TSG6, HA, and IalphaI were crucial factors for the settlement and probably the subsequent differentiation of MSCs. PMID: 26178374
    12. BM-MSCs injected into mice with colitis do not localize to the intestine but instead form aggregates in the peritoneum where they produce immunoregulatory molecules, including TSG6, that reduce intestinal inflammation. PMID: 25790743
    13. This study demonstrated that following stimulation with TNF-alpha, MSCs modulate microglia activity through TSG-6. PMID: 25088370
    14. suggest that TSG-6 plays an important role in MSCs-mediated immunosuppressive effect on DC PMID: 25014173
    15. This study provides insight into what we believe to be a previously undescribed multifaceted role of mesenchymal stem cell-released TSG-6 in wound healing. PMID: 23921952
    16. TSG-6 is implicated in the experimental murine model of allergic pulmonary inflammation and is likely to contribute to the pathogenesis of asthma PMID: 23118230
    17. TSG-6 increases the accumulation of HA in the cell-associated matrix, partially by preventing its dissolution from the cell-associated matrix into the conditioned medium, but primarily by inducing HA synthesis PMID: 23129777
    18. analysis of irreversible heavy chain transfer to hyaluronan oligosaccharides by tumor necrosis factor-stimulated gene-6 PMID: 23166324
    19. cells expressing CD44/TSG-6 failed to roll on hyaluronan, instead, they stuck and remained "tethered" to the substrate under fluid flow PMID: 12011075
    20. observations identify tumor necrosis factor-alpha-stimulated gene 6 as a target of prostaglandin action and show that its production in ovulatory follicles is associated with proper formation of the cumulus-derived extracellular matrix PMID: 12586778
    21. results provide clear evidence that TNFIP6 is a key catalyst in the formation of the cumulus extracellular matrix and indispensable for female fertility PMID: 12668637
    22. TSG-6 mRNA is induced in cumulus cells in culture by cAMP and that the secreted TSG-6 protein is a key structural component of the mouse cumulus cell-oocyte complex matrix. PMID: 12959984
    23. Tnsf6 plays the dominant role in both WT (WildType) and KO (connexin43-null) mice heart. PMID: 14756057
    24. The ability of TSG-6 to modulate the interaction of hyaluronan with CD44 has important implications for CD44-mediated cell activity at sites of inflammation, where TSG-6 is expressed. PMID: 15060082
    25. TSG-6 protein forms stable complexes with hyaluronan and binds to chondroitin sulfates PMID: 15699048
    26. The tumor necrosis factor alpha induced protein 6 is as a potential marker that could be used for early diagnosis and prognosis of cancerous or precancerous lesions. PMID: 16467113
    27. Tumor necrosis factor alpha-induced protein 6 (TNFAIP6) is essential for catalyzing heavy chains of inter-alpha-trypsin inhibitor to be covalently linked to hyaluronan in expanding mouse oocyte-cumulus cell complexes. PMID: 18256330
    28. TSG-6 has dual roles in bone remodeling: it inhibits RANKL-induced bone erosion in inflammatory diseases, and its interactions with BMP-2 and RANKL help to balance mineralization by osteoblasts and bone resorption by osteoclasts PMID: 18586671
    29. Results describe the expression and cellular localization of TSG-6 protein in islets of female non-obese diabetic (NOD) mice using frozen section immunofluorescence staining. PMID: 18979174

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  • 数据库链接：

    KEGG: mmu:21930

    STRING: 10090.ENSMUSP00000069231

    UniGene: Mm.3509