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  4. Mouse Thyroid-Peroxidase,TPO ELISA Kit

Mouse Thyroid-Peroxidase,TPO ELISA Kit

  • 中文名称：
    小鼠甲状腺过氧化物酶(TPO)酶联免疫试剂盒
  • 货号：
    CSB-E08353m
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse TPO ELISA Kit was designed for the quantitative measurement of Mouse TPO protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Competitive ELISA kit, its detection range is 2.5 ng/mL-40 ng/mL and the sensitivity is 2.5 ng/mL.

  • 别名：
    Tpo ELISA Kit; Thyroid peroxidase ELISA Kit; TPO ELISA Kit; EC 1.11.1.8 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    2.5 ng/mL-40 ng/mL
  • 灵敏度：
    2.5 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<15%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse TPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 90    
    Range % 86-103    
    1:2 Average % 89    
    Range % 82-97    
    1:4 Average % 95    
    Range % 87-100    
    1:8 Average % 90    
    Range % 83-99    
  • 回收率：
    The recovery of mouse TPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 94 87-102    
    EDTA plasma (n=4) 95 90-103    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average      
    40 0.272 0.263 0.268      
    20 0.525 0.530 0.528      
    10 0.894 0.874 0.884      
    5 1.334 1.377 1.356      
    2.5 2.554 2.528 2.541      
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4).
  • 基因功能参考文献：
    1. Lnk Deficiency Leads to TPO-Mediated Osteoclastogenesis and Increased Bone Mass Phenotype PMID: 28067429
    2. Hearing impairment in hypothyroid dwarf mice caused by mutations of the thyroid peroxidase gene. PMID: 24297261
    3. A novel hypothyroid dwarfism is due to the missense mutation Arg479Cys of the thyroid peroxidase gene in the mouse. PMID: 16762971
  • 亚细胞定位：
    Membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Peroxidase family, XPO subfamily
  • 数据库链接：

    KEGG: mmu:22018

    STRING: 10090.ENSMUSP00000021005

    UniGene: Mm.4991