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Mouse Spondin-1(SPON1) ELISA kit

  • 中文名称：
    小鼠脊椎蛋白-1(SPON1)酶联免疫试剂盒
  • 货号：
    CSB-EL022599MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse SPON1 ELISA Kit was designed for the quantitative measurement of Mouse SPON1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

  • 别名：
    Spon1 ELISA Kit; Spondin-1 ELISA Kit; F-spondin ELISA Kit
  • 缩写：
    SPON1
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse SPON1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 102
    Range % 97-106
    1:2 Average % 104
    Range % 99-108
    1:4 Average % 86
    Range % 81-92
    1:8 Average % 94
    Range % 89-98
  • 回收率：
    The recovery of mouse SPON1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 102 97-106
    EDTA plasma (n=4) 87 83-92
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    10 2.249 2.342 2.296 2.142
    5 1.472 1.587 1.530 1.376
    2.5 0.925 0.964 0.945 0.791
    1.25 0.591 0.607 0.599 0.445
    0.625 0.392 0.386 0.389 0.235
    0.312 0.301 0.311 0.306 0.152
    0.156 0.218 0.222 0.220 0.066
    0 0.153 0.154 0.154 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Cell adhesion protein that promotes the attachment of spinal cord and sensory neuron cells and the outgrowth of neurites in vitro. May contribute to the growth and guidance of axons in both the spinal cord and the PNS.
  • 基因功能参考文献：
    1. We conclude that upon ligand binding, TLR4 activates PI3K/Akt signaling to induce F-spondin expression, subsequently control CREB-mediated IL-6 production to promote VSMC migration. These findings provide vital insights into the essential role of F-spondin in VSMC function and will be valuable for developing new therapeutic strategies against atherosclerosis. PMID: 27091427
    2. These findings demonstrated novel roles for F-spondin and IL-1Ra in type B CpG oligodeoxynucleotides-mediated cell proliferation and migration of macrophages. PMID: 26042735
    3. Spon1 deletion leads to a local and systemic reduction of TGF-beta levels resulting in increased BMP signaling and increased bone deposition in adult mice. PMID: 24875054
    4. F-spondin is expressed in embryonic cartilage, where it has the capacity to enhance chondrocyte terminal differentiation and mineralization. PMID: 20839318
  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 数据库链接：