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  4. Mouse Semaphorin-3D(SEMA3D) ELISA kit

Mouse Semaphorin-3D(SEMA3D) ELISA kit

  • 中文名称：
    小鼠臂板蛋白-3D(SEMA3D)酶联免疫试剂盒
  • 货号：
    CSB-EL020983MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse SEMA3D ELISA Kit was designed for the quantitative measurement of Mouse SEMA3D protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.8 pg/mL .

  • 别名：
    Sema3d ELISA Kit; Semaphorin-3D ELISA Kit
  • 缩写：
    SEMA3D
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    23.5 pg/mL-1500 pg/mL
  • 灵敏度：
    5.8 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse SEMA3D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %87
    Range %84-95
    1:2Average %95
    Range %91-98
    1:4Average %99
    Range %96-102
    1:8Average %96
    Range %89-103
  • 回收率：
    The recovery of mouse SEMA3D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8680-94
    EDTA plasma (n=4)9892-104
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    15002.433 2.332 2.383 2.224
    7502.049 1.928 1.989 1.830
    3751.339 1.318 1.329 1.170
    187.50.727 0.741 0.734 0.575
    940.485 0.496 0.491 0.332
    470.280 0.291 0.286 0.127
    23.50.221 0.225 0.223 0.064
    00.159 0.158 0.159 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Induces the collapse and paralysis of neuronal growth cones. Could potentially act as repulsive cues toward specific neuronal populations. Binds to neuropilin.
  • 基因功能参考文献：
    1. s did not find evidence for genetic interaction between Ret and Sema3d affecting survival, presence of myenteric plexus or intestine transcriptome. PMID: 28334784
    2. Data indicate that annexin A2 (AnxA2) regulates the secretion of semaphorin 3D (Sema3D) from pancreatic ductal adenocarcinoma (PDA) cells. PMID: 26243191
    3. Semaphorin 3d requires neuropilin 1 or PI3K/Akt, whereas semaphorin 3e requires plexin D1 in directing endothelial motility. PMID: 24825896
    4. In the absence of Sema3d, endothelial tubes form in a region that is normally avascular PMID: 23685842
    5. Two proepicardial markers (Scleraxis and Semaphorin3D) were identified that genetically delineate heretofore uncharacterized proepicardial subcompartments. PMID: 22421048
    6. Sema 3D and Sema 3F genes are overlappingly and distinctly expressed in the developing parathyroid and thymus, respectively, and they might play roles in the interactions between these organs and surrounding tissues PMID: 18489001

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Semaphorin family
  • 数据库链接：