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  4. Mouse SPARC-like protein 1(SPARCL1) ELISA kit

Mouse SPARC-like protein 1(SPARCL1) ELISA kit

  • 中文名称：
    小鼠SPARC样蛋白1(SPARCL1)酶联免疫试剂盒
  • 货号：
    CSB-EL022487MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse SPARCL1 ELISA Kit was designed for the quantitative measurement of Mouse SPARCL1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 78 pg/mL-5000 pg/mL and the sensitivity is 19.5 pg/mL .

  • 别名：
    Sparcl1 ELISA Kit; Ecm2 ELISA Kit; Sc1 ELISA Kit; SPARC-like protein 1 ELISA Kit; Extracellular matrix protein 2 ELISA Kit; Matrix glycoprotein Sc1 ELISA Kit
  • 缩写：
    SPARCL1
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    78 pg/mL-5000 pg/mL
  • 灵敏度：
    19.5 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse SPARCL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %90
    Range %86-93
    1:2Average %95
    Range %90-101
    1:4Average %94
    Range %90-97
    1:8Average %97
    Range %95-100
  • 回收率：
    The recovery of mouse SPARCL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9490-97
    EDTA plasma (n=4)9591-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    50002.588 2.542 2.565 2.416
    25001.788 1.745 1.767 1.618
    12501.079 1.066 1.073 0.924
    6250.698 0.701 0.700 0.551
    312.50.443 0.434 0.439 0.290
    1560.298 0.278 0.288 0.139
    780.222 0.230 0.226 0.077
    00.150 0.148 0.149 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 基因功能参考文献：
    1. tumor endothelial cells heterogeneity is regulated by SPARCL1, which promotes the cell quiescence and vessel homeostasis contributing to the favorable prognoses associated with Th1-tumor microenvironments colorectal carcinomas. PMID: 27721236
    2. Study shows that astrocyte-secreted hevin is a trans-synaptic linker that bridges presynaptic NRX1alpha with postsynaptic NL1B. This way, hevin organizes both pre- and postsynaptic specializations and aligns them across the synapse. PMID: 26771491
    3. Hevin is transiently expressed in the IrrPTK-injured corneas and loss of hevin predisposes them to aberrant wound healing. PMID: 24303054
    4. SC1 and SPARC may share significant homology but are likely to have distinct but complementary roles in central nervous system development. PMID: 22173850
    5. This study presented evidence that combined NL1 and SC1 overexpression triggers excitotoxic neurodegeneration through SC1 signaling at synaptic connections initiated by NL1. PMID: 22542674
    6. In gliomas implanted into murine brain, a SLF was associated with SPARC in the neovasculature but not with hevin, the latter prominent in the astrocytes encompassed by infiltrating tumor PMID: 21688302
    7. These results identify hevin as a positive and SPARC as a negative regulator of synapse formation. PMID: 21788491
    8. Hevin does not appear to be critical to regulating intraocular pressure. Hevin is expressed in trabecular meshwork but, in contrast to SPARC, does not appear to be regulated by TGF-beta2. PMID: 21220554
    9. proteolysis of hevin by ADAMTS4 in the mouse cerebellum is important for the normal development of this tissue PMID: 20018883
    10. determination of collagen I binding and examination of expression in a variety of tissues PMID: 12538579
    11. This hematopoietic stem cell enhancer ia bound by transription factors in stem cells and in hemangioblasts. PMID: 17962413
    12. Data show that Sparcl1 in the locus Rgcs1 region is highly expressed in the ganglion cell layer and optic nerve head. PMID: 18671875

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  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 蛋白家族：
    SPARC family
  • 组织特异性：
    Highest expression in brain. Moderate levels in heart, adrenal gland, epididymis and lung. Low levels in kidney, eye, liver, spleen, submandibular gland and testis.
  • 数据库链接：