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  4. Mouse Retinol-binding protein,RBP ELISA Kit

Mouse Retinol-binding protein,RBP ELISA Kit

  • 中文名称：
    小鼠视黄醇结合蛋白(RBP)酶联免疫试剂盒
  • 货号：
    CSB-E09151m
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    This mouse RBP4 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of mouse RBP4 in multiple samples, including serum, plasma, urine, or tissue homogenates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the RBP4 levels in the sample and is measured at 450 nm through a microplate reader.

    RBP4 is the major transporter of the hydrophobic molecule retinol or vitamin A in circulation. It transports retinol from the liver to the peripheral tissues. Plasma RBP4 levels positively correlate with retinol levels. RBP4 also transports adipokine and fatty acid. Studies have found that RBP4 functions as a hepatokine and mediates the effects of the molecular clock on glucose metabolism in mice. RBP4 is implicated in a variety of human conditions that include impaired vision and ocular diseases, disorders of glucose and lipid homeostasis, and cardiovascular diseases.

  • 别名：
    Rbp4 ELISA Kit; Retinol-binding protein 4 ELISA Kit; Plasma retinol-binding protein ELISA Kit; PRBP ELISA Kit; RBP ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, urine, tissue homogenates
  • 检测范围：
    0.312 μg/mL-20 μg/mL
  • 灵敏度：
    0.078 μg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse RBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 92    
    Range % 86-96    
    1:2 Average % 95    
    Range % 90-103    
    1:4 Average % 88    
    Range % 85-93    
    1:8 Average % 87    
    Range % 83-92    
  • 回收率：
    The recovery of mouse RBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 99 93-104    
    EDTA plasma (n=4) 94 90-97    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    μg/ml OD1 OD2 Average      
    20 0.157 0.145 0.151      
    10 0.259 0.264 0.262      
    5 0.369 0.385 0.377      
    2.5 0.587 0.545 0.566      
    1.25 0.830 0.823 0.827      
    0.625 1.278 1.233 1.256      
    0.312 1.646 1.696 1.671      
    0 2.622 2.745 2.684      
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Retinol-binding protein that mediates retinol transport in blood plasma. Delivers retinol from the liver stores to the peripheral tissues. Transfers the bound all-trans retinol to STRA6, that then facilitates retinol transport across the cell membrane.
  • 基因功能参考文献：
    1. RBP4 is involved in all-trans retinoic acid-induced cleft palate. PMID: 28849085
    2. RBP4-induced inflammation is largely mediated by TLR4. PMID: 28400700
    3. New insights into ghrelin cell physiology, and given the known functions of RBP4 and TTR, support an emerging role for the ghrelin cell in blood glucose handling and metabolism. PMID: 23840311
    4. Retinal degeneration in RBP4-Tg mice is RBP4-dependent and light-independent. PMID: 28813718
    5. Rbp4-deficient mice accumulated retinol in the liver but it was undetectable in the serum, indicating an inverse relation between serum and liver retinol levels. RBP4 is critical for the mobilization of retinol from hepatic storage pools, and such mobilization is necessary for ocular development and visual function. PMID: 26974396
    6. Hepatocytes Are the Principal Source of Circulating RBP4 in Mice PMID: 27797907
    7. RBP4 may be a critical modulator promoting the vicious cycle of insulin resistance and heart failure by activating TLR4/MyD88-mediated inflammatory pathways. Potentially, lowering RBP4 might break the vicious cycle and improve both insulin resistance and cardiac hypertrophy. PMID: 27100622
    8. Data (including data from studies in knockout mice) suggest that Rbp4 (plasma retinol-binding protein 4) is critical for antigen presentation and activation of CD4-positive T-lymphocyte in development of insulin resistance in mice obese due to high-fat diet. PMID: 26936962
    9. A novel mechanism for circadian regulation of RBP4, but also a critical role of RBP4, acting as a hepatokine in the regulation of glucose metabolism by the circadian clock. PMID: 26564180
    10. Elevated serum RBP4 raises BP. PMID: 25911613
    11. These data show that decreasing circulating TTR levels or altering TTR-RBP4 binding could be a potential therapeutic approach for the treatment of type 2 diabetes. PMID: 25524914
    12. findings suggest that RBP4 impaired in vivo adipogenesis, partly through the repression of the insulin pathway PMID: 24590924
    13. The data implicate the involvement of chondrocytic Rbp4 in bone growth, particularly in the formation of the secondary ossification center of the limb. PMID: 23224267
    14. STRA6 in tissues other than the eye appears to be the coupling of circulating holo-RBP levels to cell signaling, in turn regulating key processes such as insulin response. PMID: 23839944
    15. expression of RBPR2 in liver and fat suggests a possible role in mediating established metabolic actions of RBP4 in those tissues PMID: 23105095
    16. TTR blocks the ability of holo-retinol-binding protein to associate with STRA6 and thereby effectively suppresses both STRA6-mediated retinol uptake and STRA6-initiated cell signaling. PMID: 22826435
    17. Suggest that RBP4 may be involved in the dyslipidemia associated with polycystic ovary syndrome in nonobese adolescents and that there may be an independent relationship between RBP4 and triglycerides but not between RBP4 and insulin resistance. PMID: 22341881
    18. RBP4 is not only an adipocytokine, but also a hepatic cytokine leading to metabolic syndrome, non-alcoholic fatty liver disease and type 2 diabetes. PMID: 21983273
    19. results indicate that rimonabant may improve vascular function by modulating RBP4 along with pro-inflammatory cytokines PMID: 21585349
    20. Basal and cyclic AMP-induced Rbp4 transcription is regulated by a multiprotein complex that is similar to ones that modulate expression of genes of steroid hormone biosynthesis. PMID: 19389484
    21. RBP4 is an adipocyte-derived 'signal' that may contribute to the pathogenesis of type 2 diabetes PMID: 16034410
    22. Lowering transthyretin(TTR) levels or interfering with RBP4-TTR binding may enhance insulin sensitivity in obesity and type 2 diabetes PMID: 18285525
    23. These results reveal a selective effect of all-trans retinoic acid inhibiting RBP4 expression specifically in adipocytes. PMID: 18769064
    24. Identification and characterization of a non-retinoid ligand for retinol-binding protein 4 which lowers serum retinol-binding protein 4 levels in vivo. PMID: 19147488
    25. HMGA1 is an important modulator of RBP4 gene expression in vivo. PMID: 19460132
    26. Although mice lacking HMGA1 are diabetic and express very low levels of the insulin receptor, they have increased insulin sensitivity. Changes in circulating Rbp4 might partly account for this paradox. [REVIEW] PMID: 19664187

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Calycin superfamily, Lipocalin family
  • 数据库链接：

    KEGG: mmu:19662

    STRING: 10090.ENSMUSP00000025951

    UniGene: Mm.2605