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  4. Mouse Regenerating islet-derived protein 3-gamma(REG3G) ELISA kit

Mouse Regenerating islet-derived protein 3-gamma(REG3G) ELISA kit

  • 中文名称：
    小鼠再生胰岛衍生蛋白3γ(REG3G）酶联免疫试剂盒
  • 货号：
    CSB-EL019549MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse REG3G ELISA Kit was designed for the quantitative measurement of Mouse REG3G protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

  • 别名：
    Reg3g ELISA Kit; Pap3 ELISA Kit; Regenerating islet-derived protein 3-gamma ELISA Kit; REG-3-gamma ELISA Kit; Pancreatitis-associated protein 3 ELISA Kit; Regenerating islet-derived protein III-gamma ELISA Kit; Reg III-gamma) [Cleaved into: Regenerating islet-derived protein 3-gamma 16.5 kDa form; Regenerating islet-derived protein 3-gamma 15 kDa form] ELISA Kit
  • 缩写：
    REG3G
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse REG3G in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:100Average %93
    Range %89-106
    1:200Average %92
    Range %83-100
    1:400Average %95
    Range %89-99
    1:800Average %97
    Range %89-103
  • 回收率：
    The recovery of mouse REG3G spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9491-98
    EDTA plasma (n=4)9792-105
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.190 2.123 2.157 1.996
    5001.793 1.762 1.778 1.617
    2501.295 1.257 1.276 1.115
    1250.779 0.748 0.764 0.603
    62.50.562 0.554 0.558 0.397
    31.250.375 0.368 0.372 0.211
    15.60.263 0.254 0.259 0.098
    00.157 0.165 0.161
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

平均分:
5.0分 - 1 个评价

样品类型：Serum ,细胞培养上清液

样品信息：小鼠

稀释比：其他

产品评价： 我使用Mouse Reg3genRating islet-derived protein 3-gamma（REG3G)ELISA kit，检测小鼠血清内REG3G,分别检测了1：50，1：100，1：200的稀释比例。以1：200为例，对照组和实验组的OD值分别是0.2603和0.4635，实验结果符合我们对实验前期的预测。产品评价：该elisa检测试剂盒产品质量过关，检测效果好。能较好的满足实验需求。

By 吴老师

靶点详情

  • 功能：
    Bactericidal C-type lectin which acts exclusively against Gram-positive bacteria and mediates bacterial killing by binding to surface-exposed carbohydrate moieties of peptidoglycan. Restricts bacterial colonization of the intestinal epithelial surface and consequently limits activation of adaptive immune responses by the microbiota. The uncleaved form has bacteriostatic activity, whereas the cleaved form has bactericidal activity against L.monocytogenes and methicillin-resistant S.aureus. Regulates keratinocyte proliferation and differentiation after skin injury.
  • 基因功能参考文献：
    1. the secretion of regenerating islet-derived IIIgamma (RegIIIgamma) is responsible for the protective effect of Adipose-derived stem cells. PMID: 26866937
    2. The study shows that cardiac stress activates Reg3gamma expression and p38 MAPK is an upstream regulator of Reg3gamma gene expression in heart. Altogether the data suggest Reg3gamma is associated with cardiac inflammatory signaling. PMID: 27798352
    3. regenerating islet-derived gene gamma promotes beta cell regeneration and preserves beta cells from autoimmunity damage by increasing regulatory T cell differentiation and inducing tolerated dendritic cells. PMID: 26667474
    4. Recombinant IFN-I or IFN-I expression induced by RIG-I promoted growth of intestinal organoids in vitro and production of the antimicrobial peptide regenerating islet-derived protein 3 gamma (RegIIIgamma). PMID: 28424327
    5. REG3gamma-deficient mice have altered mucus distribution and increased mucosal inflammatory responses to the microbiota and enteric pathogens in the ileum. PMID: 24345802
    6. antibacterial function for lung epithelium through Stat3-mediated induction of Reg3gamma. PMID: 23401489
    7. Reg-IIIgamma expression is mainly observed in epithelial cells of the airways and intestine, but not in the nervous system; expression levels show a gradually increasing pattern along with development. PMID: 21681751
    8. Letter: aggregation status of PAP1 and PAP2 could change their role in regulating the inflammation in acute pancreatitis. PMID: 21926556
    9. findings show that RegIIIgamma is essential for maintaining a ~50-micrometer zone that physically separates the microbiota from the small intestinal epithelial surface PMID: 21998396
    10. findings show that RegIIIgamma and its human counterpart, HIP/PAP, are directly antimicrobial proteins that bind their bacterial targets via interactions with peptidoglycan carbohydrate PMID: 16931762
    11. antibacterial activities of mouse RegIIIgamma and its human ortholog, HIP/PAP, are tightly controlled by an inhibitory N-terminal prosegment that is removed by trypsin in vivo. PMID: 19095652

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  • 亚细胞定位：
    Secreted. Cytoplasm.
  • 组织特异性：
    Predominantly expressed in the small intestine, including Paneth cells (at protein level). Hardly detectable in the colon (at protein level). Highly expressed in the lung epithelium during methicillin-resistant S.aureus infection (at protein level). Skin
  • 数据库链接：