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  4. Mouse Pulmonary surfactant-associated protein A,SP-A ELISA Kit

Mouse Pulmonary surfactant-associated protein A,SP-A ELISA Kit

  • 中文名称：
    小鼠肺表面活性物质相关蛋白A(SP-A)酶联免疫试剂盒
  • 货号：
    CSB-E08685m
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse SFTPA1 ELISA Kit was designed for the quantitative measurement of Mouse SFTPA1 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL .

  • 别名：
    Sftpa1 ELISA Kit; Sftp-1 ELISA Kit; Sftp1 ELISA Kit; Sftpa ELISA Kit; Pulmonary surfactant-associated protein A ELISA Kit; PSAP ELISA Kit; PSP-A ELISA Kit; SP-A ELISA Kit
  • 缩写：
    SFTPA1
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    3.12 pg/mL-200 pg/mL
  • 灵敏度：
    0.78 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse SP-A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1000Average %84
    Range %80-92
    1:2000Average %96
    Range %91-105
    1:4000Average %98
    Range %93-103
    1:8000Average %93
    Range %86-98
  • 回收率：
    The recovery of mouse SP-A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9087-95
    EDTA plasma (n=4)9390-95
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    2002.637 2.714 2.676 2.549
    1002.331 2.368 2.350 2.223
    501.847 1.901 1.874 1.747
    251.273 1.267 1.270 1.143
    12.50.845 0.869 0.857 0.730
    6.250.542 0.583 0.563 0.436
    3.120.322 0.317 0.320 0.193
    00.128 0.126 0.127 　
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 功能：
    In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration. Enhances the expression of MYO18A/SP-R210 on alveolar macrophages.
  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix. Secreted, extracellular space, surface film.
  • 蛋白家族：
    SFTPA family
  • 数据库链接：