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Mouse Protein disulfide-isomerase(P4HB) ELISA kit

  • 中文名称：
    小鼠蛋白质二硫键异构酶(P4HB)酶联免疫试剂盒
  • 货号：
    CSB-EL017342MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse P4HB ELISA Kit was designed for the quantitative measurement of Mouse P4HB protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

  • 别名：
    P4hb ELISA Kit; Pdia1Protein disulfide-isomerase ELISA Kit; PDI ELISA Kit; EC 5.3.4.1 ELISA Kit; Cellular thyroid hormone-binding protein ELISA Kit; Endoplasmic reticulum resident protein 59 ELISA Kit; ER protein 59 ELISA Kit; ERp59 ELISA Kit; Prolyl 4-hydroxylase subunit beta ELISA Kit; p55 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse P4HB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 94
    Range % 87-98
    1:2 Average % 103
    Range % 96-108
    1:4 Average % 92
    Range % 85-97
    1:8 Average % 101
    Range % 95-107
  • 回收率：
    The recovery of mouse P4HB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 89 84-95
    EDTA plasma (n=4) 99 91-105
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    20 2.248 2.175 2.212 2.044
    10 1.615 1.584 1.600 1.432
    5 1.016 0.967 0.992 0.824
    2.5 0.545 0.536 0.541 0.373
    1.25 0.342 0.358 0.350 0.182
    0.625 0.251 0.243 0.247 0.079
    0.312 0.215 0.210 0.213 0.045
    0 0.171 0.165 0.168 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration.
  • 基因功能参考文献：
    1. Unfolded protein response caused by protein misfolding, may lead to PDI-dependent NOX activation and contribute to neurotoxicity in neurodegenerative diseases including ALS. PMID: 23118353
    2. PDI from endothelial cells is required for fibrin generation in vivo. PMID: 20668226
    3. Protein disulfide isomerase (PDI) on the platelet surface is recognized by anti-dengue virus NS1 antibodies. PMID: 19822367
  • 亚细胞定位：
    Endoplasmic reticulum. Endoplasmic reticulum lumen. Melanosome. Cell membrane; Peripheral membrane protein.
  • 蛋白家族：
    Protein disulfide isomerase family
  • 数据库链接：