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  4. Mouse Protein-arginine deiminase type-4(PADI4) ELISA kit

Mouse Protein-arginine deiminase type-4(PADI4) ELISA kit

  • 中文名称：
    小鼠精氨酸脱亚胺酶4(PADI4)酶联免疫试剂盒
  • 货号：
    CSB-EL017379MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse PADI4 ELISA Kit was designed for the quantitative measurement of Mouse PADI4 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 4.7 pg/mL-300 pg/mL and the sensitivity is 1.17 pg/mL.

  • 别名：
    Padi4 ELISA Kit; Pad4 ELISA Kit; Pdi4 ELISA Kit; Protein-arginine deiminase type-4 ELISA Kit; EC 3.5.3.15 ELISA Kit; Peptidylarginine deiminase IV ELISA Kit; Protein-arginine deiminase type IV ELISA Kit
  • 缩写：
    PADI4
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    4.7 pg/mL-300 pg/mL
  • 灵敏度：
    1.17 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Epigenetics and Nuclear Signaling
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse PADI4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %91
    Range %85-99
    1:2Average %95
    Range %89-101
    1:4Average %96
    Range %92-110
    1:8Average %93
    Range %86-102
  • 回收率：
    The recovery of mouse PADI4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9788-105
    EDTA plasma (n=4)9689-104
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    3002.228 2.230 2.229 2.051
    1501.457 1.378 1.418 1.240
    750.854 0.822 0.838 0.660
    37.50.483 0.513 0.498 0.320
    18.750.355 0.363 0.359 0.181
    9.40.274 0.259 0.267 0.089
    4.70.233 0.226 0.230 0.052
    00.175 0.181 0.178
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. Citrullinates histone H1 at 'Arg-54' (to form H1R54ci), histone H3 at 'Arg-2', 'Arg-8', 'Arg-17' and/or 'Arg-26' (to form H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively) and histone H4 at 'Arg-3' (to form H4R3ci). Acts as a key regulator of stem cell maintenance by mediating citrullination of histone H1: citrullination of 'Arg-54' of histone H1 (H1R54ci) results in H1 displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance. Promotes profound chromatin decondensation during the innate immune response to infection in neutrophils by mediating formation of H1R54ci. Required for the formation of neutrophil extracellular traps (NETs); NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation. Citrullination of histone H3 prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1.
  • 基因功能参考文献：
    1. PAD4 in neutrophils is essential for kidney NET formation and ischemic kidney injury. PMID: 29061334
    2. PADi4 is critical for neutrophil extracellular traps formation, but is dispensable for generalized citrullination PMID: 28188029
    3. sFlt-1 overexpression in Padi4(-/-) mice resulted in dramatically lower inflammatory and thrombotic response, which was accompanied by significant reduction in pregnancy losses. Inhibition of NETosis may serve as a novel target in disorders of impaired placentation. PMID: 28007693
    4. a role for NETs in cardiac fibrosis and conclude that PAD4 regulates age-related organ fibrosis and dysfunction. PMID: 28031479
    5. PADI4 exacerbates arthritis with diverse immunological modifications. PMID: 26293116
    6. Selective inhibition of peptidylarginine deiminase IV with Cl-amidine blocked NET formation, reduced atherosclerotic lesion area, and delayed time to carotid artery thrombosis in a photochemical injury model. PMID: 24425713
    7. Padi4 is part of the pluripotency transcriptional network, binding to regulatory elements of key stem-cell genes and activating their expression PMID: 24463520
    8. PAD4-mediated chromatin decondensation in the neutrophil is crucial for pathological venous thrombosis and present neutrophil activation and PAD4 as potential drug targets for deep vein thrombosis. PMID: 23650392
    9. PAD4 is dispensable in this effector phase model of arthritis PMID: 22551352
    10. analysis of regulation of histone modification and chromatin structure by the p53-PADI4 pathway PMID: 22334079

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Cytoplasmic granule.
  • 蛋白家族：
    Protein arginine deiminase family
  • 组织特异性：
    Expressed in pluripotent embryonic stem and induced pluripotent stem cells but not multipotent neural stem cells.
  • 数据库链接：