Mouse Protein Wnt-5a(WNT5A) ELISA kit

Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression. Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells. Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes.

1. Study shows that the interplay between CYR61-CTGF-NOV1 (CCN1) and Wnt5a in endothelial cells and pericytes determines the angiogenic outcome in a model of ischemic retinopathy. Data highlight the significance of CCN1-EC and CCN1-pericyte communication signals in driving physiological and pathological angiogenesis. PMID: 28469167
2. Activating effect of WNT5A regulated bone formation in response to hindlimb unloading in mice, and pretreatment with WNT5A partly rescued the osteoporosis caused by mechanical unloading. PMID: 30007964
3. calcineurin is a key regulator of Wnt5a-induced AQP2 activation without affecting intracellular cAMP level and PKA activity. PMID: 27892464
4. The findings demonstrate that induction of Vangl protein phosphorylation plays an essential role in transducing Wnt5a signaling to establish Planar cell polarity (PCP) in mammalian development, suggesting a phosphorylation-regulated "Vangl activity gradient" model in addition to the well-documented "Fz activity gradient" model in Wnt/PCP signaling. PMID: 29056748
5. Post-mitotic filopodial "pathfinding" is guided by mesenchymal WNT5A. Without WNT5A, some cells fail to tether basally and undergo apoptosis, leading to a shortened midgut. PMID: 30016620
6. We demonstrate with genetic evidence that the Wnt5a gradient acts as a global cue that is instructive in establishing planar cell polarity (PCP) in the limb mesenchyme, and that Wnt5a also plays a permissive role to allow Fgf4 and Fgf8 signaling to orient PCP. PMID: 29615464
7. Butyrate and bioactive proteolytic form of Wnt-5a regulate colonic epithelial proliferation and spatial development. PMID: 27561676
8. These data suggest that Wnt5a modulates the development of arthritis by promoting inflammation and osteoclast fusion, and provide the first mouse genetic evidence of a role for endogenous Wnt5a in autoimmune disease. PMID: 28724439
9. Here, we show that deletion of a single Wnt family member, Wnt5a, is sufficient to elicit profound disruptions in synaptic plasticity, structural maintenance, and learning and memory in adult mice, identifying the importance of this particular noncanonical Wnt in later-life functions. PMID: 28069946
10. Wnt5a promotes IL-6 and TIMP-1 release from mouse cardiac fibroblasts. PMID: 28357477
11. bivalent histone codes during odontogenic differentiation PMID: 28880717
12. WNT5A promoted spermatogonial stem cells activity both in vitro and in vivo. PMID: 27509137
13. results indicate that Wnt5a signaling restricts infection by antimony drug-sensitive and -resistant Leishmania donovani strains, at least in part by prohibiting parasite niche formation within host macrophages PMID: 28659356
14. During mouse embryonic stem cell (ESC) in vitro osteogenesis, the noncanonical WNT5a is expressed early on. PMID: 26956615
15. This study shows that Wnt5a is not required for development of the renal medulla and that loss of the renal medullary region in the Wnt5a-deleted kidney is caused by an abnormal ureter-bladder connection. PMID: 27923152
16. WNT5A in the bone marrow niche is required to regenerate hematopoietic stem cells and leukemic cells with functional ability to rearrange the actin cytoskeleton and engraft successfully. PMID: 27998927
17. This study describes the localization and functional role of WNT-5A in human and mouse fibrotic livers. Hepatic WNT-5A expression parallels collagen type I expression. In vivo and in vitro, the myofibroblasts were identified as the key hepatic cells producing WNT-5A. WNT-5A is under control of TGF-beta and its activities are primarily profibrotic. PMID: 28057611
18. we confirmed the requirement of Wnt5a in the deferoxamine-mediated osteoblast-promoting effects by analyzing the matrix mineralization of Wnt5a-deficient cells. The promoting effect of deferoxamine on matrix mineralization in wild-type cells was completely abolished in Wnt5a(-/-) cells. PMID: 27540134
19. opposing gradients of Wnt5a and Wnt5b and of their Sfrp inhibitors, together with intercellular signaling via planar cell polarity proteins, polarize node cells along the anterior-posterior axis for breaking of left-right symmetry. PMID: 28292423
20. Wnt5a is responsible for maintaining effective communication between the stromal and epithelial compartments partly through the action of luminal and stromal secreted factors, Fgf10 and Ihh. PMID: 27965056
21. findings reveal that FZD9 and heterotrimeric G proteins regulate Wnt-5a signaling and dendritic spines in cultured hippocampal neurons. PMID: 27402827
22. Here we show that an evolutionarily conserved genomic region named AS3_9 comprises three TEs (AmnSINE1, X6b_DNA and MER117), inserted side-by-side, and functions as a distal enhancer for wnt5a expression during morphogenesis of the mammalian secondary palate. PMID: 27741242
23. we here reported that Wnt5a mediated activation of canonical Wnt signaling pathway might contribute to the orthodontic force induced bone remodeling. PMID: 27456852
24. Wnt5a deficiency can regulate inflammatory cytokine secretion, polarization, and apoptosis in MTB-infected macrophage PMID: 27828711
25. These results showed that Wnt5a could promote the maturation of mast cells via the canonical Wnt signaling pathway and provide important insights into the molecular mechanisms underlying the differentiation of mast cells PMID: 27843938
26. the CaMKK-CaMKIalpha cascade is required for the axonal growth effect of Wnt5a during neuronal polarization. PMID: 26772170
27. indicate that restricted expression of Wnt5a in the caudal SpM is essential for normal OFT morphogenesis, and uncover a novel function of spatially regulated cell cohesion by Wnt5a in driving the deployment of SHF cells from the SpM into the OFT PMID: 26916252
28. Findings indicate wingless-related MMTV integration site 5A protein (WNT5a) as a key regulator of follicle development and gonadotropin responsiveness. PMID: 26667040
29. The results in a potent increase in odontoblastic cell differentiation, indicating the unique involvement of Atg5, autophagy and Wnt5 signaling in CS/BMP-4-induced differentiation of ES cells into odontoblast-like cells, at a relatively early stage. PMID: 26806855
30. the Wnt5a/Frizzled-2 axis suppresses beta-catenin signaling in hepatocytes in an autocrine manner, thereby contributing to timely conclusion of the liver regeneration process PMID: 26100214
31. effects on hypoxic pulmonary hypertension by regulating pulmonary vascular remodeling and right ventricular hypertrophy via reduction in beta-catenin/cyclin D1 signaling PMID: 25956683
32. Wnt5a regulates the pathogenesis of gp120-induced pain. PMID: 25840108
33. Study identified a novel Wnt5a/Fzd4 signaling pathway that contributes to the regulation of dendrite morphogenesis PMID: 25424568
34. The Wnt5a-Ror2 axis promotes the signaling circuit between interleukin-12 and interferon-gamma in colitis PMID: 26030277
35. study presents the finding that activation of the NF-kappaB pathway by Porphyromonas endodontalis LPS is important for Wnt5a expression in osteoblasts, and that TRIB3 may modulate the sustained expression of Wnt5a in osteoblasts stimulated by P. endodontalis PMID: 25601649
36. Wnt5a and its receptors are sorted to their correct destination by different mechanisms and that the basolateral secretion of Wnt5a is necessary for apical lumen formation in the epithelial cyst. PMID: 25593127
37. In Wnt5a null mice, second heart field (SHF) progenitors are trapped in the SHF and fail to be deployed to the outflow tract (OFT) efficiently, resulting in a reduction in the inferior OFT myocardial wall and subpulmonary myocardium. PMID: 25410658
38. Wnt5a abrogated the inhibitory effects of Wnt16 on Rankl-induced osteoclastogenesis PMID: 26093292
39. Therefore, Wnt5a-induced noncanonical signaling cooperates with Wnt/beta-catenin signaling to achieve proper bone formation. PMID: 24670389
40. findings demonstrate, for the first time, that Wnt5a reduces the accumulation of cholesterol in lipid-loaded cells by regulating the mRNA expression of Caveolin-1 and ABCA1, which are involved in reverse cholesterol transport. PMID: 24827906
41. There is an important role of Wnt5a in kidney development. Disrupted Wnt5a results in kidney cysts in zebrafish and pleiotropic abnormal kidney development in mice. PMID: 25412793
42. Skin of Wnt5a TG mice was not psoriatic, but characterized with normal proliferation and homeostasis of epidermis. Instead, the TG mice displayed impaired hair follicle transition from telogen to anagen, likely due to impaired canonical Wnt signalling. PMID: 25219536
43. Non-canonical Wnt5a/Ror2 signaling regulates kidney morphogenesis by controlling intermediate mesoderm extension. PMID: 25082826
44. Adenovirus-mediated Wnt5a silencing in vivo attenuates the development of atherosclerotic disease. PMID: 25050997
45. Wnt5a through Wnt/JNK signaling alone or both Wnt/JNK and Wnt/PKC signaling promoted the differentiation of mMSCs into type II alveolar epithelial cells and the migration of mMSCs. PMID: 24658098
46. Wnt5a regulates HSC quiescence and hematopoietic repopulation through the Ryk receptor and that this process is mediated by suppression of reactive oxygen species. PMID: 23939973
47. The activation of Wnt5a-Ror2 signaling in epithelial cells undergoing epithelial-to-mesenchymal transition (EMT) may play an important role in disrupting TBM via MMP-2 induction during renal fibrosis. PMID: 23755735
48. The results indicate an important role of Wnt5a-Ror2 signaling in morphogenesis of the metanephric mesenchyme to ensure proper epithelial tubular formation of the ureteric bud required for kidney development. PMID: 24891614
49. Wnt5a-mediated Ryk activation triggers divergent responses in callosal axons and dendrites in the in vitro context. PMID: 24471468
50. Studied expression of Wnt family member 5A in murine hair cycle and its inhibitory effects on follicle in vivo. PMID: 24507677

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Secreted, extracellular space, extracellular matrix. Secreted.

Wnt family

Expressed in a gradient at the caudal end of the embryo during gastrulation and later in the distal-most aspect of several structures that extend from the body such as the limbs and genital tubercle.

KEGG: mmu:22418

STRING: 10090.ENSMUSP00000064878

UniGene: Mm.287544