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  4. Mouse Protein Wnt-10b(WNT10B) ELISA kit

Mouse Protein Wnt-10b(WNT10B) ELISA kit

  • 中文名称：
    小鼠Wnt-10b蛋白(WNT10B)酶联免疫试剂盒
  • 货号：
    CSB-EL026130MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse WNT10B ELISA Kit was designed for the quantitative measurement of Mouse WNT10B protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 9.4 pg/mL-600 pg/mL and the sensitivity is 2.35 pg/mL.

  • 别名：
    Wnt10b ELISA Kit; Wnt-10b ELISA Kit; Wnt10 ELISA Kit; Wnt12 ELISA Kit; Protein Wnt-10b ELISA Kit; Protein Wnt-12 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    9.4 pg/mL-600 pg/mL
  • 灵敏度：
    2.35 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Stem Cells
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse WNT10B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 85  
    Range % 81-89  
    1:2 Average % 99  
    Range % 95-103  
    1:4 Average % 88  
    Range % 84-93  
    1:8 Average % 104  
    Range % 97-106  
  • 回收率：
    The recovery of mouse WNT10B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 87 84-90  
    EDTA plasma (n=4) 102 98-106  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    600 2.048 2.154 2.101 1.943  
    300 1.388 1.398 1.393 1.235  
    150 0.745 0.714 0.730 0.572  
    75 0.404 0.435 0.420 0.262  
    37.5 0.300 0.321 0.311 0.153  
    18.75 0.264 0.278 0.271 0.113  
    9.38 0.205 0.209 0.207 0.049  
    0 0.159 0.157 0.158    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Member of the Wnt ligand gene family that encodes for secreted proteins, which activate the Wnt signaling cascade. Specifically activates canonical Wnt/beta-catenin signaling and thus triggers beta-catenin/LEF/TCF-mediated transcriptional programs. Involved in signaling networks controlling stemness, pluripotency and cell fate decisions. Acts in the immune system, mammary gland, adipose tissue, bone and skin.
  • 基因功能参考文献：
    1. Treatment of secondary hyperparathyroidism with calcitriol improved the bone anabolism by inhibiting osteoclasts and promoting osteoblasts that might be achieved by increasing the Wnt 10b level. PMID: 30149605
    2. Wnt10b recruitment to lipid rafts and mTOR activation promotes regeneration of injured optic nerve. PMID: 28683327
    3. We examined a new culture condition for DPCs including addition of Wnt-10b, which promoted proliferation and maintained their HF induction ability for more than ten passages. These results suggest that Wnt-10b plays a pivotal role in proliferation and maintenance of DPCs in vitro PMID: 27032939
    4. Wnt10b expression was assessed by flow cytometry under estrogen- and TNFalpha-deficient conditions. PMID: 28800644
    5. AT2R inhibits adipogenic differentiation in mesenchymal stem cells. Moreover, this inhibitory effect is associated with Wnt10b/beta-catenin signaling. PMID: 27295344
    6. XBP1s can regulate Wnt10b by a post-transcriptional mechanism through directly inducing microRNA-148a. PMID: 27055562
    7. findings suggest that Wnt10b plays an important role in regulating asthmatic airway inflammation through modification of the T cell response and is a prospective target in the disease process PMID: 26436894
    8. Data show that Wnt protein Wnt10b is expressed in cardiomyocytes and localized in the intercalated discs of mouse and human hearts. PMID: 26338900
    9. Oleuropein protects mice from gh-fat diet induced adiposity by upregulation of genes involved in WNT10b-mediated signaling and downregulation of genes involved in galanin-mediated signaling cascades. PMID: 25104077
    10. results suggest that Wnt-10b represses tumor cell properties, such as proliferation and migration of B16 melanoma cells, driving them toward a more differentiated state along a melanocyte lineage PMID: 26056007
    11. Wnt10b/DKK1 can modulate hair follicle size during hair regeneration. PMID: 24750467
    12. In senescent osteopenic mice, blocking the CADH2-Wnt interaction using an antagonist peptide increased Wnt5a and Wnt10b expression, bone formation, and bone mass. PMID: 24664975
    13. Hypoxia-inducible factor-2alpha-dependent hypoxic induction of Wnt10b expression in adipogenic cells. PMID: 23900840
    14. Data suggest that Tgfb1 (transforming growth factor beta 1) induces Wnt10b in osteoclasts; induction of Wnt10b may enhance restoration of bone lost during bone turnover and may explain osteoclast stimulation of osteoblast-directed mineralization. PMID: 23861379
    15. Wnt-10b promoted cell proliferation and trichogenesis, while Wnt-3a showed those abilities to a limited extent PMID: 23916705
    16. Wnt10b promotes the differentiation of melanocytes by activating canonical Wnt signaling in melanocytes PMID: 23569433
    17. Ex vivo analysis showed decreased basal and Wnt3a-induced Wnt5a and Wnt10b mRNA expression in FHL2-deficient bone marrow cells, further indicating that this defect may contribute to the reduced osteoblast function in FHL2 deficient mice PMID: 23201222
    18. Direct binding of XBP1 to the Wnt10b promoter and the subsequent decrease of the beta-catenin signalling pathway represent a novel adipogenic differentiation mechanism. PMID: 23603388
    19. It was shown that Wnt10b induces hair follicle regeneration in vivo via the enhanced activation of the canonical Wnt signaling pathway. In Wnt10b-induced hair follicles, beta-catenin was translocated to the nucleus. PMID: 22832493
    20. direct PPR signaling in T cells plays an important role in PTH-induced bone anabolism by promoting T-cell production of Wnt10b PMID: 22393015
    21. Lysophosphatidic acid treatment dose dependently reduces lipid accumulation and PPARgamma mRNA expression and increases Wnt10b ligand mRNA expression in differentiated 3T3-L1 cells. PMID: 21999426
    22. Leucine-rich Amelogenin Peptide (LRAP) treatment elevates the Wnt10b expression level whereas Wnt10b knockdown by siRNA abrogates the effect of LRAP. PMID: 21663957
    23. reduction of Wnt10b in muscle with a rotator cuff tear is a key signal in fatty degeneration of the muscle PMID: 21246616
    24. results indicate that Wnt10b is uniquely required for maintenance of mesenchymal progenitor activity in adult bone PMID: 20499361
    25. SREBP-1c and Wnt10b display inverse expression patterns during muscle ontogenesis and regeneration, as well as during satellite cells differentiation. PMID: 20041157
    26. the formation of the mammary line expressing Wnt10b is the earliest discernible ectodermal event in murine embryonic mammary gland development PMID: 14745960
    27. Wnt10b inhibits development of brown adipose tissue PMID: 15190075
    28. myoblasts isolated from Wnt10b null mice showed increased adipogenic potential, likely contributing to excessive lipid accumulation in actively regenerating myofibers in vivo in Wnt10b-/- mice PMID: 15673614
    29. Wnt10b-/- mice have decreased trabecular bone and serum osteocalcin, confirming that Wnt10b is an endogenous regulator of bone formation PMID: 15728361
    30. Wnt-10b promotes the differentiation of mouse-derived primary skin epithelial cells PMID: 16469292
    31. These results suggest that Wnt-10b promotes the differentiation of MPSEC and may play an important role in hair follicle development by promoting differentiation of epithelial cells. PMID: 16510119
    32. Adipogenic potential can be activated during muscle regeneration through increased adipogenic signaling in conjunction with decreased Wnt10b signaling. PMID: 17487458
    33. suggest a promoting effect of Wnt-10b on hair shaft growth that is involved with stimulation of the dermal papilla via Wnt-10b/beta-catenin signalling PMID: 17548054
    34. High bone mass in Oc-Wnt10b mice is primarily caused by increased osteoblastogenesis, with a minor contribution from elevated mineralizing activity of osteoblasts. PMID: 17708715
    35. These data place Wnt/beta-catenin signaling upstream of key morphogenetic signaling pathways at multiple stages of tooth development. PMID: 18022614
    36. phenotypic shift in mesenchymal progenitor cell commitment was associated with reciprocal regulation of early adipogenic and osteoblastogenic transcription factors and with a reduction in Wnt7b and Wnt10b mRNA and beta-catenin protein levels PMID: 18045882
    37. Our results suggest that Wnt-10b is unique and plays an important role in differentiation of epithelial cells in the hair follicle. PMID: 18155657
    38. During the regenerative phase, both hematopoietic and stromal cell elements within the bone marrow microenvironment show increased expression of Wnt10b, which can function to enhance growth of hematopoietic precursors. PMID: 18308947
    39. Metastatic osteosarcoma cell lines showed better chemotaxis response to Wnt10B than the non-metastatic osteosarcoma cell lines PMID: 18465804
    40. cAMP stimulates phosphorylation and binding of CREB to the cyclin D1 gene promoter; simultaneously, hypermethylation of the Wnt10b gene promoter suppresses binding of CREB, allowing adipogenesis to proceed PMID: 18957421
    41. S1P induces osteoblast precursor recruitment and promotes mature cell survival. Wnt10b and BMP6 also were significantly increased in mature osteoclasts, whereas sclerostin levels decreased during differentiation. PMID: 19075223

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  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix. Secreted.
  • 蛋白家族：
    Wnt family
  • 组织特异性：
    Expressed in embryos and in the mammary gland of non-pregnant mice.
  • 数据库链接：

    KEGG: mmu:22410

    STRING: 10090.ENSMUSP00000023732

    UniGene: Mm.4709