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  4. Mouse Prolactin/Luteotropic Hormone(PRL/LTH) ELISA Kit

Mouse Prolactin/Luteotropic Hormone(PRL/LTH) ELISA Kit

  • 中文名称：
    小鼠催乳素(PRL/LTH)酶联免疫试剂盒
  • 货号：
    CSB-E06882m
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    The mouse prolactin (PRL) ELISA Kit is used to quantitatively measure PRL concentrations in mouse serum, plasma, or tissue homogenates. It performs well in important characteristics, including sensitivity, specificity, precision, recovery, linearity, and lot-to-lot consistency. This assay is based on the competitive ELISA mechanism and enzyme-substrate chromogenic reaction. The solution color develops inversely to the amount of PRL in the sample. And the intensity of the color can be measured at 450 nm via a microplate reader.

    PRL is a sex hormone that is mainly synthesized and secreted by the lactotrophs of the pituitary and is responsible for the induction of lactation, the development of mammary alveoli, and milk production. It also exerts immunoregulatory properties. PRL signals by binding to PRL and activates a signaling cascade, including Jak/Stat, MAPK, Src, and PI3K/Akt pathways. PRL levels normally increase during pregnancy and breastfeeding and may rise due to physical stress, exercise, and certain medications and diseases. Higher-than-normal levels of PRL can cause certain symptoms, such as irregular periods, infertility, and erectile dysfunction.

  • 别名：
    Prl ELISA Kit; Prolactin ELISA Kit; PRL ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.8 ng/mL-28 ng/mL
  • 灵敏度：
    0.5 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse PRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 97  
    Range % 92-101  
    1:2 Average % 93  
    Range % 90-96  
    1:4 Average % 95  
    Range % 89-102  
    1:8 Average % 96  
    Range % 90-102  
  • 回收率：
    The recovery of mouse PRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 89-100  
    EDTA plasma (n=4) 96 89-103  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average 　  
    25 0.165 0.174 0.170 　  
    4.69 0.352 0.374 0.363 　  
    0.78 0.573 0.589 0.581 　  
    0.22 1.026 1.048 1.037 　  
    0.06 1.562 1.574 1.568 　  
    0 2.205 2.233 2.219 　  
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Prolactin acts primarily on the mammary gland by promoting lactation.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Somatotropin/prolactin family
  • 数据库链接：