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Mouse Plastin-2(LCP1) ELISA kit

  • 中文名称：
    小鼠网素-2(LCP1)酶联免疫试剂盒
  • 货号：
    CSB-EL012824MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse LCP1 ELISA Kit was designed for the quantitative measurement of Mouse LCP1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.25 pg/mL.

  • 别名：
    Lcp1 ELISA Kit; Pls2 ELISA Kit; Plastin-2 ELISA Kit; 65 kDa macrophage protein ELISA Kit; L-plastin ELISA Kit; Lymphocyte cytosolic protein 1 ELISA Kit; LCP-1 ELISA Kit; pp65 ELISA Kit
  • 缩写：
    LCP1
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    25 pg/mL-1600 pg/mL
  • 灵敏度：
    6.25 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse LCP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %94
    Range %90-98
    1:2Average %99
    Range %94-103
    1:4Average %84
    Range %80-88
    1:8Average %90
    Range %86-94
  • 回收率：
    The recovery of mouse LCP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8984-93
    EDTA plasma (n=4)106101-113
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1OD2AverageCorrected
    16002.612 2.537 2.575 2.434
    8002.142 2.056 2.099 1.958
    4001.471 1.567 1.519 1.378
    2000.874 0.905 0.890 0.749
    1000.534 0.579 0.557 0.416
    500.290 0.279 0.285 0.144
    250.229 0.249 0.239 0.098
    00.144 0.137 0.141
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Actin-binding protein. Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. Modulates the cell surface expression of IL2RA/CD25 and CD69.
  • 基因功能参考文献：
    1. the actin-bundling protein L-plastin (LPL) is required for the perinatal development of alveolar macrophages. PMID: 27758872
    2. Results identify L-plastin as a key effector of Mst1 and establish a novel mechanism linking a signaling intermediate to an actin-binding protein critical to T cell migration. PMID: 27465533
    3. L-plastin modulates both T- and B-cell function during the germinal center reaction and the production of T-cell-dependent antibody responses. PMID: 23589339
    4. L-plastin may participate in signaling that enables lymphocyte transmigration PMID: 21832165
    5. LPL-dependent actin bundling facilitates the formation of lamellipodia and normal immunological synapses and thereby enables T cell activation. PMID: 21076065
    6. Regulation of sealing ring formation by L-plastin and cortactin in osteoclasts. PMID: 20650888
    7. We thus identify L-plastin as a molecule critical for CCR7-mediated t-cell motility but dispensable for early CCR7 signaling. PMID: 20194718
    8. plastin plays a role in host defense distinguishes integrin signaling from cell adhesion and spreading. PMID: 12871642

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  • 亚细胞定位：
    Cytoplasm, cytoskeleton. Cell projection. Cell junction. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side.
  • 数据库链接：