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  4. Mouse Plasma protease C1 inhibitor(SERPING1) ELISA kit

Mouse Plasma protease C1 inhibitor(SERPING1) ELISA kit

  • 中文名称：
    小鼠血浆蛋白酶C1抑制剂(SERPING1)酶联免疫试剂盒
  • 货号：
    CSB-EL021086MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse SERPING1 ELISA Kit was designed for the quantitative measurement of Mouse SERPING1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 5.6 ng/mL-360 ng/mL and the sensitivity is 1.4 ng/mL.

  • 别名：
    Serping1 ELISA Kit; C1nh ELISA Kit; Plasma protease C1 inhibitor ELISA Kit; C1 Inh ELISA Kit; C1Inh ELISA Kit; C1 esterase inhibitor ELISA Kit; C1-inhibiting factor ELISA Kit; Serpin G1 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    5.6 ng/mL-360 ng/mL
  • 灵敏度：
    1.4 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Blood Coagulation
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse SERPING1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1000 Average % 100  
    Range % 95-105  
    1:2000 Average % 104  
    Range % 101-107  
    1:4000 Average % 83  
    Range % 80-86  
    1:8000 Average % 98  
    Range % 95-103  
  • 回收率：
    The recovery of mouse SERPING1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 88 85-92  
    EDTA plasma (n=4) 96 93-99  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    360 2.559 2.569 2.564 2.461  
    180 2.253 2.298 2.276 2.173  
    90 1.822 1.843 1.833 1.730  
    45 1.079 1.098 1.089 0.986  
    22.5 0.602 0.634 0.618 0.515  
    11.25 0.331 0.351 0.341 0.238  
    5.6 0.205 0.220 0.213 0.110  
    0 0.102 0.104 0.103    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Activation of the C1 complex is under control of the C1-inhibitor. It forms a proteolytically inactive stoichiometric complex with the C1r or C1s proteases. May play a potentially crucial role in regulating important physiological pathways including complement activation, blood coagulation, fibrinolysis and the generation of kinins. Very efficient inhibitor of FXIIa. May inhibit chymotrypsin and kallikrein.
  • 基因功能参考文献：
    1. C1INH prevented myocardial ischemia reperfusion injury in mice. PMID: 22705194
    2. Transgenic mice have been generated that express human C1 inhibitor mRNA and protein under the control of the human promoter and regulatory elements; C1nh production is demonstrated in mouse brain, spleen, liver, heart, kidney, and lung. PMID: 12421980
    3. In vivo, C1 inhibitor (C1INH) reduces the number of viable bacteria in the blood and peritoneal fluid and accelerates killing of bacteria by blood neutrophils and peritoneal macrophages. PMID: 17785834
    4. C1-inhibitor limits neointimal plaque formation and inflammation. This may involve blockade of complement activation, inhibition of leukocyte recruitment, and reduced triglyceride levels PMID: 18071075
    5. In addition to the protective activities mediated via inhibition of the complement system, these studies indicate that C1INH also plays a direct role in suppression of leukocyte transmigration into reperfused tissue. PMID: 18787060

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Serpin family
  • 数据库链接：