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  4. Mouse Phenylalanine-4-hydroxylase(PAH) ELISA kit

Mouse Phenylalanine-4-hydroxylase(PAH) ELISA kit

  • 中文名称：
    小鼠苯丙氨酸羟化酶4(PAH)酶联免疫试剂盒
  • 货号：
    CSB-EL017396MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse PAH ELISA Kit was designed for the quantitative measurement of Mouse PAH protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.8 pg/mL.

  • 别名：
    Pah ELISA Kit; Phenylalanine-4-hydroxylase ELISA Kit; PAH ELISA Kit; EC 1.14.16.1 ELISA Kit; Phe-4-monooxygenase ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    23.5 pg/mL-1500 pg/mL
  • 灵敏度：
    5.8 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse PAH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 104
    Range % 101-109
    1:2 Average % 95
    Range % 91-99
    1:4 Average % 86
    Range % 83-89
    1:8 Average % 92
    Range % 87-96
  • 回收率：
    The recovery of mouse PAH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 86 83-89
    EDTA plasma (n=4) 89 85-96
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1500 2.646 2.701 2.674 2.481
    750 2.063 2.000 2.032 1.839
    375 1.324 1.405 1.365 1.172
    187.5 0.826 0.834 0.830 0.637
    94 0.593 0.587 0.590 0.397
    47 0.362 0.371 0.367 0.174
    23.5 0.278 0.284 0.281 0.088
    0 0.192 0.194 0.193 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

Our customer would like to know if you have cross-reactivity data for the CSB-EL017396MO PAH ELISA kit (Mouse).
They are curious to know whether you have tested to see if this mouse kit cross-reacts with human.
Thanks for your assistance.

A:
Thanks for your inquiry!
For different species, the primary structure and the advanced structure of proteins are different. The antibody's characteristics are also different. So we didn't validate human samples with mouse kit before. We don't suggest you use this kit to test human samples. Sorry we don't have human pah ELisa kit.
Q:

What is the contents of the antibody in this kit?

A:
Thanks for your inquiry!
Capture antibody is goat polyclonal antibody and detection antibody is goat polyclonal antibody.

靶点详情

  • 功能：
    Catalyzes the hydroxylation of L-phenylalanine to L-tyrosine.
  • 基因功能参考文献：
    1. We propose that CNS monoamine deficiency may be the cause of the partially reversible adverse behavioral effects associated with chronic HPA in Pah(enu2) mice, but that phenylalanine-reducing treatments initiated during adulthood are unable to correct the neuropathology and attendant cognitive deficits that develop during juvenile life in late-treated Pah(enu2/enu2) mice PMID: 29331172
    2. we assessed DNA methylation patterns in brain tissues using methylated DNA immunoprecipitation and paired end sequencing in adult PAH(enu2) animals maintained under either continuous dietary Phe restriction or chronic hyperphenylalaninemia. Heterozygous PAH(enu2/WT) litter mates served as controls for normal Phe exposure PMID: 26822703
    3. These observations support the proposition that differences in phenylalanine hydroxylase activity underlie the variation in S-carboxymethyl-L-cysteine sulfoxidation and that no other enzyme is able to undertake this reaction. PMID: 26338263
    4. A single injection of PAH-RBCs was able to decrease Phe levels by nearly 80% in normal mice PMID: 23867524
    5. high levels of Phe, such as in phenylketonuria, are unlikely to directly induce brain hypomyelination, suggesting involvement of alternative mechanisms in this myelination defect PMID: 20151197
    6. phenylalanine concentration regulates the activity of PAH in the hph-1 mouse and that this acts via a mechanism that includes phosphorylation of the PAH molecule PMID: 19560382
    7. Tetrahydrobiopterin protects PAH activity in vivo. PMID: 15556637
    8. low level of phenylalanine clearance was observed in mice expressing PAH and GTP-CH from the keratin 14 promoter PMID: 15946242

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  • 相关疾病：
    Mouse strains deficient in phenylalanine hydroxylase (Pah) were created as models of phenylketonuria (PKU).
  • 蛋白家族：
    Biopterin-dependent aromatic amino acid hydroxylase family
  • 数据库链接：