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  4. Mouse Osteoclast-associated immunoglobulin-like receptor(OSCAR) ELISA kit

Mouse Osteoclast-associated immunoglobulin-like receptor(OSCAR) ELISA kit

  • 中文名称：
    小鼠破骨细胞相关免疫球蛋白样受体(OSCAR)酶联免疫试剂盒
  • 货号：
    CSB-EL017255MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    The Mouse Osteoclast-associated immunoglobulin-like receptor (OSCAR) ELISA kit is a reliable and accurate tool for the detection of OSCAR protein levels in mouse serum, plasma, and tissue homogenates. OSCAR is a type I transmembrane protein that is predominantly expressed on osteoclasts and plays a crucial role in bone resorption.

    The Mouse OSCAR ELISA kit has a wide detection range of 37.5 pg/mL-2400 pg/mL and a high sensitivity of 9.375 pg/mL, ensuring precise and accurate measurements even at low concentrations. The kit uses a sandwich assay principle, which involves the use of two monoclonal antibodies specific to the mOSCAR protein.

    The assay time for the kit is 1-5 hours, and the sample volume required is 50-100ul. Detection is done at a wavelength of 450 nm, making it easy to read and interpret the results. The kit is suitable for use in immunology research, where OSCAR protein levels are of interest, and has been cited in over 2 research articles.

  • 别名：
    Oscar ELISA Kit; Osteoclast-associated immunoglobulin-like receptor ELISA Kit; Osteoclast-associated receptor ELISA Kit; mOSCAR ELISA Kit; Ocl178 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    37.5 pg/mL-2400 pg/mL
  • 灵敏度：
    9.375 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse OSCAR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:5 Average % 89  
    Range % 82-93  
    1:10 Average % 105  
    Range % 97-108  
    1:20 Average % 93  
    Range % 88-97  
    1:40 Average % 95  
    Range % 91-98  
  • 回收率：
    The recovery of mouse OSCAR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 89-97  
    EDTA plasma (n=4) 91 85-94  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    2400 2.885 2.877 2.881 2.761  
    1200 2.068 2.089 2.079 1.959  
    600 1.152 1.123 1.138 1.018  
    300 0.689 0.687 0.688 0.568  
    150 0.398 0.391 0.395 0.275  
    75 0.301 0.289 0.295 0.175  
    37.5 0.210 0.222 0.216 0.096  
    0 0.119 0.121 0.120    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Regulator of osteoclastogenesis which plays an important bone-specific function in osteoclast differentiation.
  • 基因功能参考文献：
    1. OSCAR expression in monocytic cells is regulated by proatherogenic stimuli further pointing towards a role in vascular inflammation or plaque vulnerability during atherosclerosis PMID: 23817038
    2. Data suggest that OSCAR is a collagen receptor that binds to specific collagen motifs and costimulates osteoclastogenesis in DAP12-deficient humans and mice. PMID: 21841309
    3. PU.1 and MITF transcription factors synergistically activate OSCAR gene expression, which is further enhanced by the TRANCE-induced MKK6/p38 signaling cascade. PMID: 12695521
    4. the FcRgamma-mediated signal transduction by OSCAR is involved in osteoclast differentiation PMID: 15184345
    5. analysis of a positive feedback circuit of TRANCE-induced activation of NFATc1, involving NFATc1-mediated OSCAR expression and its subsequent activation of NFATc1, necessary for efficient differentiation of osteoclasts PMID: 16109714
    6. Results indicate that USFs 1 and 2 serve as modulators in the induction of OSCAR and RANKL-mediated osteoclastogenesis. PMID: 18762192

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  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Leukocyte receptor complex/polymeric immunogobulin receptor (PIR/LRC) family
  • 组织特异性：
    Specifically expressed in preosteoclasts or mature osteoclasts.
  • 数据库链接：