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Mouse Lipopolysaccharide-binding protein(LBP) ELISA kit

  • 中文名称：
    小鼠脂多糖结合蛋白(LBP)酶联免疫试剂盒
  • 货号：
    CSB-EL012775MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This mouse LBP ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of mouse LBP in serum, plasma, and tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of LBP bound in the initial step.

    LBP is an acute-phase protein mainly produced in hepatocytes that binds to bacterial lipopolysaccharide (LPS) thus initiating an innate host response. LBP combines with LPS and then recruits CD14 and MD-4/2 complex, eventually leading to the activation of signal transduction pathways and the generation of cytokines and other pro-inflammatory mediators. Circulating LBP exerts immunologic function in a concentration-dependent manner. Subnormal concentrations of LBP augment phagocytosis and scavenging of LPS from blood. However, high levels of LBP decrease the release of pro-inflammatory cytokines. LBP is a potential acute-phase biomarker for monitoring the progress of severe sepsis, infectious endocarditis, and cardiovascular disease.

  • 别名：
    LbpLipopolysaccharide-binding protein ELISA Kit; LBP ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    12.5 ng/mL-800 ng/mL
  • 灵敏度：
    3.12 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse LBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %87
    Range %82-92
    1:2Average %102
    Range %97-107
    1:4Average %98
    Range %94-101
    1:8Average %93
    Range %90-96
  • 回收率：
    The recovery of mouse LBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8985-93
    EDTA plasma (n=4)9691-102
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    8002.922 2.974 2.948 2.803
    4002.166 2.159 2.163 2.018
    2001.447 1.448 1.448 1.303
    1000.913 0.925 0.919 0.774
    500.524 0.565 0.545 0.400
    250.335 0.312 0.324 0.179
    12.50.253 0.258 0.256 0.111
    00.140 0.150 0.145
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Plays a role in the innate immune response. Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. Acts as an affinity enhancer for CD14, facilitating its association with LPS. Promotes the release of cytokines in response to bacterial lipopolysaccharide.
  • 基因功能参考文献：
    1. The LBP gene is a macrophage-specific LXR target that promotes foam cell survival and atherogenesis. PMID: 24671012
    2. LBP is a critical factor in the development of non-alcoholic fatty liver disease. PMID: 27404046
    3. LBP is a proinflammatory soluble adipokine that acts as a brake for adipogenesis, strengthening the negative effects of palmitate and LPS on adipocyte differentiation. PMID: 26201685
    4. Differential expression of LBP and TGFB1, along with other genes, in different mesenchymal stromal cells preparations, produces the variable responses to external stimuli. PMID: 24664302
    5. findings describe a novel role for LBP in normal hippocampal development and raise the possibility that some of the behavioral sequelae of early life stress are mediated by reduced expression of LBP during a critical period of neurodevelopment. PMID: 21993208
    6. The amino acid sequence for two mimic epitopes of the inflammatory site of LBP were determined to be WKAQKRFMKKSG and LKTRKLFWKYKD. PMID: 20833181
    7. LBP plays an important role in early alcohol-induced liver injury by enhancing LPS-induced signal transduction, most likely in Kupffer cells. PMID: 11884468
    8. LBP plays an important role in resistance to lethal Salmonella peritonitis (but not to oral or intravenous infection) by facilitating the role of neutrophils in mediating acute inflammation. PMID: 12055258
    9. role LBP plays in local pulmonary immune defenses to bacterial challenge PMID: 12353926
    10. In the case of intraperitoneal infection of LBP-deficient mice with Salmonella typhimurium, no local inflammatory response is evident, neutrophil influx is delayed, and the mice succumb to the infection. PMID: 12370383
    11. plays an essential role in the innate immune response to Gram-positive pneumococci PMID: 12932360
    12. Locally produced LBP is an essential component of an effective innate immune response to E. coli peritonitis. PMID: 14638760
    13. LBP is one of the critical molecules regulating the acute and chronic airway response to inhaled lipopolysaccharides (LPS). PMID: 15356561
    14. LBP knockout mice are protected from toxicity with a decrease in hepatic necrosis following acetaminophen challenge. This suggests a novel role for LBP in modulating acetaminophen-induced liver injury. PMID: 15619225
    15. LBP does not contribute to an effective host response in Mycobacterium tuberculosis infection in mice. PMID: 15993364
    16. LBP per se possesses a protective effect on acute necrotizing pancreatitis PMID: 16163057
    17. In conclusion, hemorrhagic shock and resuscitation to mice cause severe, LBP-mediated hepatocellular damage. PMID: 16614372
    18. Oxidized phospholipid inhibition of toll-like receptor (TLR) signaling is restricted to TLR2 and TLR4: roles for CD14, LPS-binding protein, and MD2 as targets for specificity of inhibition. PMID: 18559343
    19. LPS-binding protein mediates LPS-induced liver injury and mortality in the setting of biliary obstruction. PMID: 18948440
    20. Marked concomitant increases in plasma LBP and endotoxin-like activity following murine trypanosome infection might play an important role in the pathogenesis of trypanosomosis PMID: 19022463
    21. Paneth cells express LBP together with other proteins acting in the innate immune response of the gut, such as lysozyme, defensins and intelectin. PMID: 19234712
    22. Innate defense against lethal peritoneal infection with Salmonella requires the direct proinflammatory involvement of LPS-binding protein (LBP), a major nonredundant function of LBP in this model. PMID: 11466385

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  • 亚细胞定位：
    Secreted. Cytoplasmic granule membrane.
  • 蛋白家族：
    BPI/LBP/Plunc superfamily, BPI/LBP family
  • 数据库链接：