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  4. Mouse L-lactate dehydrogenase A chain(LDH-A)ELISA Kit

Mouse L-lactate dehydrogenase A chain(LDH-A)ELISA Kit

  • 中文名称：
    小鼠L-乳酸脱氢酶A链(LDH-A)酶联免疫试剂盒
  • 货号：
    CSB-E17733m
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse LDHA ELISA Kit was designed for the quantitative measurement of Mouse LDHA protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.156 mU/mL-10 mU/mL and the sensitivity is 0.039 mU/mL.

  • 别名：
    Ldha ELISA Kit; Ldh-1 ELISA Kit; Ldh1L-lactate dehydrogenase A chain ELISA Kit; LDH-A ELISA Kit; EC 1.1.1.27 ELISA Kit; LDH muscle subunit ELISA Kit; LDH-M ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.156 mU/mL-10 mU/mL
  • 灵敏度：
    0.039 mU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse LDH-A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:100 Average % 100
    Range % 95-106
    1:200 Average % 88
    Range % 84-93
    1:400 Average % 107
    Range % 103-110
    1:800 Average % 86
    Range % 83-90
  • 回收率：
    The recovery of mouse LDH-A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 101 96-108
    EDTA plasma (n=4) 85 81-90
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mU/ml OD1 OD2 Average
    10 0.144 0.148 0.146
    5 0.215 0.211 0.213
    2.5 0.325 0.331 0.328
    1.25 0.508 0.499 0.504
    0.625 0.763 0.778 0.771
    0.312 1.230 1.241 1.236
    0.156 1.561 1.570 1.566
    0 2.180 2.034 2.107
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 基因功能参考文献：
    1. The release of lactate dehydrogenase A (LDHA) from degenerating neurons drives central nervous system (CNS) angiogenesis. PMID: 29248508
    2. Hair follicle stem cells maintain a metabolic state that allows them to remain dormant and yet quickly respond to appropriate proliferative stimuli mediated by LDH-A signaling. PMID: 28812580
    3. Results revealed that beta-arrestin-1 regulates lactate metabolism to contribute to beta2-adrenergic receptor functions in improved memory formation. PMID: 27916196
    4. The s identified lactate dehydrogenase (LDH) as a new functional target of AMPKalpha1. PMID: 28515121
    5. LDHA-associated lactic acid accumulation in melanomas inhibits tumor surveillance by T and natural killer cells. PMID: 27641098
    6. lactate dehydrogenase A (LDHA) is induced in activated T cells to support aerobic glycolysis but promotes IFN-gamma expression independently of its 3'UTR. PMID: 27708054
    7. LDHA is a direct target of miR-34a in regulating glucose metabolism and tumor growth in breast cancer. PMID: 26902416
    8. Findings suggest that RANKL protein-induced lactate dehydrogenase (LDH) activation stimulates glycolytic and mitochondrial respiratory metabolism via transcription factor NFATc1 signaling. PMID: 27077737
    9. We propose a profibrotic feed forward loop, in which radiation induces LDHA expression and lactate production, which can lead to further activation of TGF-beta to drive the fibrotic process. PMID: 26254422
    10. High LDH-A expression is associated with metastases in breast tumors. PMID: 23833310
    11. PGC-1alpha reduces the expression of LDH A and one of its regulators, the transcription factor myelocytomatosis oncogene. PMID: 23650363
    12. Lactate dehydrogenase A is overexpressed in pancreatic cancer and promotes the growth of pancreatic cancer. PMID: 23404405
    13. Down-regulation of expression of stress-activated genes PKC-alpha, c-Myc and LDH-A by alpha-tocopherol in cancerous mice. PMID: 22132835
    14. The cumulus cells and oocytes showed intense signals for lactate dehydrogenase (LDH)-A respectively. PMID: 21360229
    15. isoproterenol induces changes in gastrocnemius muscle and serum lactate dehydrogenase expression in mice PMID: 21552790
    16. Cocaine could inhibit the activities of ATPase, LDH and SDH in cultured splenocytes. PMID: 20653129
    17. The increased LDH-A/LDH-B ratio causes high brain lactate levels, which, in turn, are predictive of aging phenotypes. PMID: 21041631
    18. The muscle subunit of lactate dehydrogenase is an integral part of the sarcolemmal K(ATP) channel complex in vivo, where it couples the metabolic status of the cell with the K(ATP) channel activity essential for protection against ischemia. PMID: 12145195
    19. Reduction in LDH-A activity resulted in stimulation of mitochondrial respiration and decrease of mitochondrial membrane potential. The tumorigenicity of the LDH-A-deficient cells was severely diminished. PMID: 16766262
    20. beta-agonists increased serum LDH levels, but it was not due to release from the heart. PMID: 17853776
    21. M-LDH released from cardiomyocytes after hypoxia and reoxygenation has a role in protecting the heart from oxidative stress-induced injury through an intracellular signal transduction pathway involving ERK1/2. PMID: 18534967

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    LDH/MDH superfamily, LDH family
  • 数据库链接：