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Mouse L-amino-acid oxidase(IL4I1) ELISA kit

  • 中文名称：
    小鼠L氨基酸氧化酶(IL4I1)酶联免疫试剂盒
  • 货号：
    CSB-EL011660MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse IL4I1 ELISA Kit was designed for the quantitative measurement of Mouse IL4I1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

  • 别名：
    Il4i1 ELISA Kit; Fig1 ELISA Kit; L-amino-acid oxidase ELISA Kit; LAAO ELISA Kit; LAO ELISA Kit; EC 1.4.3.2 ELISA Kit; Interleukin-4-induced protein 1 ELISA Kit; IL4-induced protein 1 ELISA Kit; Protein Fig-1 ELISA Kit; mFIG1 ELISA Kit
  • 缩写：
    IL4I1
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL4I1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 101
    Range % 92-105
    1:2 Average % 94
    Range % 90-99
    1:4 Average % 95
    Range % 87-98
    1:8 Average % 92
    Range % 85-95
  • 回收率：
    The recovery of mouse IL4I1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 103 94-107
    EDTA plasma (n=4) 98 90-102
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1000 1.780 1.814 1.797 1.596
    500 1.316 1.301 1.309 1.108
    250 0.954 0.939 0.947 0.746
    125 0.716 0.732 0.724 0.523
    62.5 0.488 0.498 0.493 0.292
    31.2 0.406 0.400 0.403 0.202
    15.6 0.306 0.294 0.300 0.099
    0 0.203 0.199 0.201 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Secreted L-amino-acid oxidase that acts as a key immunoregulator. Has preference for L-aromatic amino acids: converts phenylalanine (Phe), tyrosine (Tyr) and tryptophan (Trp) to phenylpyruvic acid (PP), hydroxyphenylpyruvic acid (HPP), and indole-3-pyruvic acid (I3P), respectively. Also has weak L-arginine oxidase activity. Acts as a negative regulator of anti-tumor immunity by mediating Trp degradation via an indole pyruvate pathway that activates the transcription factor AHR. IL4I1-mediated Trp catabolism generates I3P, giving rise to indole metabolites (indole-3-acetic acid (IAA) and indole-3-aldehyde (I3A)) and kynurenic acid, which act as ligands for AHR, a ligand-activated transcription factor that plays important roles in immunity and cancer. AHR activation by indoles following IL4I1-mediated Trp degradation enhances tumor progression by promoting cancer cell motility and suppressing adaptive immunity. Also has an immunoregulatory function in some immune cell, probably by mediating Trp degradation and promoting downstream AHR activation: inhibits T-cell activation and proliferation, promotes the differentiation of naive CD4(+) T-cells into FOXP3(+) regulatory T-cells (Treg) and regulates the development and function of B-cells. Also regulates M2 macrophage polarization by inhibiting T-cell activation. Also has antibacterial properties by inhibiting growth of Gram negative and Gram positive bacteria through the production of NH4(+) and H2O2.
  • 基因功能参考文献：
    1. Data showed proteome changes in alveolar epithelial cells type II exposed to an infection with A. fumigatus with IL4I1 showing the most prominent increase in abundance both, in terms of relative abundance as well as activity. Also, in the infected lungs, there was levels of IL4I1 metabolic products. PMID: 28951444
  • 亚细胞定位：
    Secreted. Cytoplasmic vesicle, secretory vesicle, acrosome.; [Isoform 1]: Lysosome.; [Isoform 2]: Lysosome.
  • 蛋白家族：
    Flavin monoamine oxidase family, FIG1 subfamily
  • 组织特异性：
    Primarily found in immune tissues.; [Isoform 1]: Primarily found in immune tissues, mostly in B-lymphocytes.; [Isoform 2]: Restricted to the testis, predominantly in Sertoli cells at the periphery of the ducts, and the brain, including Purkinje cells, hip
  • 数据库链接：