Mouse Klotho ELISA Kit

May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 241 and 874, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Essential factor for the specific interaction between FGF23 and FGFR1.; The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling.

1. The present study analyzed the gene expression profile in salivary glands from accelerated aging klotho deficient mice. PMID: 28885690
2. The studies identify a role for alpha-Klotho in the regulation of muscle progenitor cell mitochondrial function and implicate alpha-Klotho declines as a driver of impaired muscle regeneration with age. PMID: 30451844
3. our study identified Klotho loss as a key event linking HDAC deregulation to the renal and bone injuries in CKD-MBD mice and demonstrated the therapeutic potentials of endogenous Klotho restoration by HDAC inhibition in treating CKD and the associated extrarenal complications. PMID: 28387374
4. Klotho has posed antioxidant and anti-apoptotic effects on oxidative injuries in TCMK-1 cells, which might be partially related to its inhibition of JNK/MAPK and p38/MAPK phosphorylation and subsequent elevation of antioxidant enzymes. Increasing Klotho expression has played a protective role against oxidative stress in tubular epithelial cells. PMID: 29285593
5. Klotho expression in the central nervous system is associated with total antioxidant capacity in mice with experimental autoimmune encephalomyelitis. PMID: 29542092
6. Using mouse strains with parathyroid-specific deletions of Klotho and CaSR and dual deletion of both genes, Klotho has a pivotal role in suppressing PTH in the absence of CaSR. Chronic hypocalcemia and ex vivo PTG culture demonstrated an independent role for Klotho in mediating PTH secretion. PMID: 29618612
7. DPP-4 inhibition with linagliptin slowed the progression of premature aging in klotho-/- mice. Provide a novel insight into the potential role of DPP-4 in the mechanism of premature aging. PMID: 29195509
8. Klotho protects tubular epithelial cells from renal ischemic-reperfusion injury and its anti-necroptotic role may be associated with oxidative stress inhibition. PMID: 29550818
9. studies uncover a novel hypomethylating character of Rhein in preventing Klotho loss and renal fibrosis, and demonstrate the efficacy of Klotho-targeted epigenetic intervention in potential treatment of renal fibrosis-associated kidney diseases. PMID: 27703201
10. the main physiological function of Klotho for mineral homeostasis in vivo is its role as co-receptor mediating Fgf23 action. PMID: 28600880
11. Findings establish a key role of osteocyte-expressed Klotho in regulating bone metabolism and indicate a new mechanism by which osteocytes control bone formation. PMID: 28396120
12. Selective HDAC3 inhibition effectively alleviated Klotho loss and kidney injury, whereas the protective effects were largely abolished when Klotho was knocked down by siRNA, suggesting that aberrant HDAC3 and Klotho loss are crucial components involved in the renal damage of mice with chronic kidney disease. PMID: 28416226
13. data showed that Klotho protects Tac-induced oxidative stress by negatively regulating the PI3K/AKT pathway and subsequently enhancing FoxO3a-mediated MnSOD expression. PMID: 28771227
14. The present study provides a novel approach for klotho gene therapy and demonstrates that direct up-regulation of klotho in the brain might improve aging-related memory impairments and decrease oxidative stress. PMID: 29544758
15. Klotho inhibits cigarette smoke-induced autophagy via down-regulation of IGF-1R, Akt, and ERK phosphorylation in alveolar macrophages. PMID: 28349330
16. In conclusion, the excessive 1,25(OH)2D3 formation in klotho-hypomorphic mice has a profound effect on murine behavior. PMID: 27109615
17. Klotho is a novel regulator of postnatal neurogenesis affecting neural stem cell proliferation and maturation sufficient to impact hippocampal-dependent spatial memory function. PMID: 28837861
18. Reduced Klotho expression aggravates tacrolimus-induced renal injury via the PI3K-Akt-FoxO pathway. PMID: 27174564
19. Two distinct isoforms of soluble Klotho appear to play counter-regulatory roles in cardiac fibrogenic responses. PMID: 27543985
20. Klotho regulates adipogenic differentiation in adipose-derived stem cells, likely via inhibition of TGFbeta1 and activation of PPAR-gamma. PMID: 26865060
21. Review of Klotho function and dysfunction in mice and humans. [Review Article] PMID: 27576165
22. Overexpression of Klotho inhibits the differentiation of RAW264.7 cells into osteoclasts. PMID: 28615093
23. Data suggest that klotho affects cognition, at least in part, by regulating hippocampal synaptic plasticity. PMID: 28215989
24. Adiponectin alters calcium and phosphate balance and renal mineral excretion, in part, through klotho. PMID: 27914707
25. These results support the notion that sialogangliosides and lipid rafts are membrane receptors for sKlotho and that the KL1 domain is sufficient for the tested biologic activities. PMID: 28442546
26. Klotho in bone is crucial for inducing FGF23 production upon renal failure PMID: 28183805
27. Results are the first to suggest an integrated regulatory function of Klotho in the distal and proximal tubules on mineral metabolism. PMID: 27292223
28. Data show that Klotho protein is present in the heart, and reduced levels of myocardial Klotho in aging mice is accompanied by lower levels heat shock protein 70 (HSP70) in the myocardium. PMID: 28152512
29. Klotho preserves AEC mtDNA integrity in the setting of oxidative stress necessary for preventing apoptosis and asbestos-induced lung fibrosis. PMID: 28428174
30. epigenetic silencing of klotho during muscular dystrophy contributes substantially to lost regenerative capacity and increased fibrosis of dystrophic muscle during late progressive stages of the disease. PMID: 27154199
31. Klotho gene deficiency promotes high-fat diet-induced fibrosis in aortic valves, likely through the AMPKalpha-RUNX2 pathway. PMID: 27242197
32. Klotho can modulate inflammation via PDLIM2/NF-kappaB p65 pathway in cyclosporine A-induced nephropathy. PMID: 28315683
33. the histological malformation in periodontal tissues in alphaKlotho-deficient mice appears to be due to not only increased concentration of inorganic phosphate but also disrupted alphaklotho/FGF23 signaling PMID: 28122194
34. Results show that Klotho regulates adrenal CYP11B2 expression. Klotho deficiency-induced spontaneous hypertension and kidney damage may be partially attributed to the upregulation of CYP11B2 expression and aldosterone synthesis. PMID: 26471128
35. Aberrant Klotho expression contributes to systemic chronic inflammation in Klotho-/- mice. PMID: 28259911
36. Thus, higher levels of FGF23 in kl/kl mouse may have a role to increase TNF-alpha production in would lesion independently of alpha-Klotho protein, and impair granulation formation and delay wound healing. PMID: 27037022
37. Klotho protein protects against diabetic nephropathy through multiple pathways. PMID: 26586006
38. In vitro studies showed interleukin-12 regulation on Klotho, while Klotho also acted as an inhibitor on interleukin-12, indicating the potential of Klotho for preserving pancreatic beta-cell function in diabetes. PMID: 27330715
39. Studies indicate that defect in Klotho gene expression results in a phenotype of premature aging. PMID: 27226196
40. beta-Klotho participates in the regulation of the peptide transporters PEPT1 and PEPT2. PMID: 27941311
41. Results show that Klotho is expressed in skeletal muscle and levels are reduced by smoking. PMID: 27021580
42. FGF23 regulates osteopontin secretion indirectly by suppressing alkaline phosphatase transcription and phosphate production in osteoblastic cells, acting through FGF receptor-3 in a Klotho-independent manner PMID: 26235988
43. Inhibition of mTOR signaling ameliorates vascular calcification via Klotho upregulation. PMID: 26061549
44. Klotho deficiency promoted HFD-induced arterial stiffening and hypertension via downregulation of AMPKalpha activity. PMID: 26781278
45. level of spontaneous remyelination was increased approximately two-fold in Klotho-overexpressing mice following cuprizone-induced demyelination. PMID: 26067431
46. data highlight the importance of ASK1/p38 MAPK pathway in the brain and identify Klotho as a possible anti-oxidant effector PMID: 26452228
47. Klotho ameliorates kidney injury and fibrosis and normalizes blood pressure by targeting the renin-angiotensin system. PMID: 26475416
48. Injection of klotho protein results in hematopoietic changes opposite to the ones observed in Klotho(-/-) mice. PMID: 24412515
49. Sodium bicarbonate administration has a favorable influence on vascular calcification and early death of klotho-deficient mice. PMID: 26538435
50. Data suggest that the level of expression of Klotho in pancreatic beta-cells regulates apoptosis; low expression (halplodeficiency of Klotho) promotes apoptosis/type 1 diabetes; beta-cell-specific overexpression prevents apoptosis/type 1 diabetes. PMID: 26340932

显示更多

收起更多

[Isoform 1]: Cell membrane; Single-pass type I membrane protein. Apical cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Secreted.; [Klotho peptide]: Secreted.

Glycosyl hydrolase 1 family, Klotho subfamily

Membrane-bound protein is present in distal renal tubules, inner ear, ependymal cells of brain choroid plexus, elongating spermatids and mature oocytes (at protein level). Soluble peptide is present in serum (100 pM) and cerebrospinal fluid. Expressed str

KEGG: mmu:16591

STRING: 10090.ENSMUSP00000077899

UniGene: Mm.6500