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  4. Mouse Kidney injury molecule 1,Kim-1 ELISA Kit

Mouse Kidney injury molecule 1,Kim-1 ELISA Kit

  • 中文名称：
    小鼠肾损伤分子1(Kim-1)酶联免疫试剂盒
  • 货号：
    CSB-E08809m-IS
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：
    CUSABIO's Kim-1 mouse ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Kim-1 in serum, plasma, tissue homogenates, or urine. This assay uses an antibody specific for mouse Kim-1 coated on a 96-well plate. Kim-1 present in a sample is bound to the wells by the immobilized antibody after adding the sample into the wells. Biotinylated Kim-1 antibody is added to the wells, forming an antibody-analyte-antibody complex. HRP-conjugated avidin is pipetted to the wells and binds to the complex. A TMB substrate solution is added to the wells and color develops in proportion to the amount of Kim-1 bound. The addition of Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm using a microplate reader.

    Kim-1 (HAVCR1) is most highly upregulated in the proximal tubular epithelium in acute and chronic kidney injury. Kim-1 functions as a phosphatidylserine receptor that recognizes and internalizes apoptotic cells and also acts as a scavenger receptor that mediates the uptake of modified low-density lipoprotein and necrotic-cell debris. A large amount of Kim-1 protein is also released into the urine, making it a useful urinary biomarker for kidney injury. In the immune system, Kim-1 is also involved in activating the differentiation of Th1, Th2, and Th17. And Kim-1 is also considered as an activating receptor in B cells, dendritic cells, and natural killer T cells.
  • 别名：
    Havcr1 ELISA Kit; Kim1 ELISA Kit; Tim1 ELISA Kit; Timd1 ELISA Kit; Hepatitis A virus cellular receptor 1 homolog ELISA Kit; HAVcr-1 ELISA Kit; Kidney injury molecule 1 ELISA Kit; KIM-1 ELISA Kit; T cell immunoglobulin and mucin domain-containing protein 1 ELISA Kit; TIMD-1 ELISA Kit; T cell membrane protein 1 ELISA Kit; T-cell immunoglobulin mucin receptor 1 ELISA Kit; TIM-1 ELISA Kit; CD antigen CD365 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, urine, tissue homogenates cell culture supernates.
  • 检测范围：
    0.78 pg/ml-50 pg/ml.
  • 灵敏度：
    0.195 pg/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Microbiology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4. May play a role in kidney injury and repair.
  • 基因功能参考文献：
    1. findings show that TIM1 is not required for hepatitis A virus (HAV) replication and pathogenesis in permissive strains of mice, although it may facilitate early stages of infection by binding phosphatidylserine on the quasi-enveloped HAV surface PMID: 28874468
    2. findings provide evidence for the role of Tim-1 in the induction of a cytokine storm phenomenon and the pathogenesis of Ebola virus disease PMID: 28951472
    3. Cisplatin enhances kidney injury molecule-1 (Kim-1) gene expression in kidney S3 cells. PMID: 27591740
    4. By preventing ERK1/2 phosphorylation following renal injury, STAT3 phosphorylation is decreased, leading to less phosphorylated STAT3 within the nucleus, and subsequently less KIM-1 mRNA increases post injury PMID: 29074644
    5. Study used a previously described highly mobile membrane mimetic membrane in combination with a conventional lipid bilayer model to generate a membrane-bound configuration of Tim1 in silico, identified two possible states for a membrane-bound form of Tim1. PMID: 28978444
    6. Our data reveal a previously unknown role for Galpha12 in regulating efferocytosis and that renal tubular epithelial cells require KIM-1 to mediate this process. PMID: 26697979
    7. Urinary L-FABP, NGAL, Kim-1 and albumin levels increased during the acute phase of kidney injury and were significantly correlated with the degree of tubulointerstitial fibrosis during the chronic phase. These markers could detect higher risk of progression to CKD. PMID: 27028054
    8. Blockade of Tim-1 changes Th1/Th2 balance and reduces circulating regulatory T cells to enhance atherosclerosis in LDL receptor knockout mice. PMID: 26821944
    9. Our results suggest that KIM-1 is an endogenous protective mechanism against renal ischemia-reperfusion injury PMID: 25759266
    10. data suggest that TIM-1 signaling plays a direct role in Breg maintenance and induction both under physiological conditions (in response to ACs) and in response to therapy through TIM-1 ligation PMID: 25645598
    11. Deletion of the mucin domain impaired KIM-1-mediated phagocytic function, resulting in increased proinflammatory cytokine production, decreased antiinflammatory growth factor secretion by proximal epithelial cells, and an increase in tissue macrophages. PMID: 25751064
    12. Tim-1 is critical for maintaining self-tolerance by regulating IL-10 production in Bregs PMID: 25582854
    13. blood biomarker that specifically reflects acute and chronic kidney injury PMID: 24904085
    14. Tim-1 expression was lower in a herpes simplex virus-induced Behcet's disease (BD) mouse model compared to that in asymptomatic BD normal (BDN) mice. PMID: 24453431
    15. Tim-1-Fc protects cardiac grafts from chronic rejection by suppressing CD4 Th17 development and functionality. PMID: 24551271
    16. KIM-1 shedding is accelerated by worsening renal cellular injury, and excess soluble KIM-1 competitively inhibits efferocytosis. PMID: 24829508
    17. Endogenous Tim-1 promotes severe systemic autoimmunity and renal disease MRL-Fas(lpr) mice. PMID: 24623145
    18. a major P-selectin ligand with a role in T cell trafficking during inflammatory responses and the induction of autoimmune disease PMID: 24703780
    19. Sustained KIM-1 expression promotes kidney fibrosis and provides a link between acute and recurrent injury with progressive chronic kidney disease. PMID: 23979159
    20. We defined a novel pathway in which TIM-1, a receptor for phosphatidylserine expressed by apoptotic cells, drives the development of asthma by sensing and responding to injured and apoptotic airway epithelial cells. PMID: 23672783
    21. TIM-1 expressing CD4 T cells are required in the mechanism of innate immune-mediated hepatic IRI in OLTs PMID: 23137033
    22. Tim-1 plays a critical role in maintaining suppressive regulatory B-cell function, while it's mucin domain plays an unexpected role in regulating Breg function and maintaining self-tolerance. PMID: 22773818
    23. Endogenous Tim-1 promotes Th1 and Th17 nephritogenic immune responses and its neutralization reduces renal injury while limiting inflammation in cell-mediated glomerulonephritis. PMID: 22205357
    24. Tim-1 functions in pathways that suppress recruitment of inflammatory cells into the airways and the generation or activity of CD4+ T cells. PMID: 22144095
    25. Tim-1 expression declined in Helicobacter pylori infection. PMID: 21923683
    26. Data show that in vivo in mice, TIM-1 is predominantly expressed on B rather than T cells, and is an inclusive marker for IL-10+ Bregs that can be induced by TIM-1 ligation. PMID: 21821911
    27. Tim1 and Tim3 are not essential for the induction of the type-2 response in lung allergy. PMID: 21470319
    28. mAb stimulation on dendritic cells regulates the balance between effector and regulatory T cells and induces Experimental autoimmune encephalitis PMID: 21469101
    29. The TIM-1:TIM-4 pathway enhances injury after renal ischemia-reperfusion injury and may be a therapeutic target. PMID: 21355054
    30. These results suggest that TIM-1 signaling in B cells augments antibody production by enhancing B cell proliferation and differentiation. PMID: 21303660
    31. results suggest that TIM-1 serves as a pattern recognition receptor on NKT cells that senses PtdSer on apoptotic cells as a damage-associated molecular pattern PMID: 20889552
    32. Tim-1 is induced on germinal centre B cells through BCR signaling. PMID: 20518819
    33. TIM1 is an endogenous ligand for LMIR5. PMID: 20566714
    34. Late upregulation of KIM-1 and NGAL could be a useful marker for sustained renal injury after acute kidney injury. PMID: 20181666
    35. TIM1 costimulation on hepatic natural killer T cells enhances cellular production of interleukin (IL)-4 while inhibiting the production of interferon (IFN)-gamma. PMID: 20220086
    36. TIM-1 regulates not only T for the role of cell activation but may also affect macrophage function in the local inflammation response. PMID: 20091883
    37. protective effects of hepatitis A virus on atopic disease depends on a common TIM-1 allele PMID: 14534576
    38. TIM-1 is a molecule that costimulates T cell activation PMID: 15793575
    39. TIM-1-TIM-4 interaction is involved in regulating T cell proliferation PMID: 15793576
    40. TIM-1 is expressed on CD4+ T cells of the lung-draining lymph nodes after intranasal immunization and plays a role in T cell activation and differentiation. PMID: 16284246
    41. These results explain the divergent immune functions described for the murine receptors and the role of TIM-1 as a cell adhesion receptor in renal regeneration and cancer. PMID: 17363299
    42. The Ig domains of murine TIM1, 3 and 4 display calcium-dependent binding to carbohydrate ligands expressed by murine splenocytes and non-murine cell lines. Both homo- and heterotypic interactions were observed for binding between the TIM proteins. PMID: 17513880
    43. Tim-1 regulates T cell responses and can alter T cell function depending on the affinity/avidity with which it is engaged. PMID: 17606630
    44. In vivo abrogation of TIM-4 or its cognate ligand TIM-1 by using a polyclonal antibody remarkably dampened Th2 differentiation and intestinal allergy. TIM-4 as a novel molecule critically required for the development of intestinal allergy. PMID: 17915221
    45. Ligation of Tim-1 in vitro effectively deprogrammed Tregs and thus produced Tregs unable to control T cell responses. PMID: 18079964
    46. TIM-4 and TIM-1 are immunologically restricted members of the group of receptors whose recognition of PS is critical for the efficient clearance of apoptotic cells and prevention of autoimmunity. PMID: 18082433
    47. These studies define previously unknown functions of TIM-1 in regulating alloimmune responses in vivo . PMID: 18172549
    48. These results demonstrate that TIM-1 is significantly increased in pulmonary tissues and PBMCs in asthmatic mice after ovalbumin challenge, and that the production of TIM-1 as well as GATA-3 is upregulated in the spleen of asthmatic mice. PMID: 18234236
    49. Ligation of Tim-1 in the presence of mature dendritic cells triggers polyclonal T cell activation. PMID: 19155484
    50. Results demonstrate that targeting T cell Ig and mucin domain-1 (Tim-1) with anti-Tim-1 overcomes transplantation tolerance resistance. PMID: 19528638

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  • 亚细胞定位：
    Membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Immunoglobulin superfamily, TIM family
  • 组织特异性：
    Expressed by stimulated T-cells. Expressed during primary antigen stimulation.
  • 数据库链接：