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  4. Mouse Interleukin 31(IL-31) ELISA Kit

Mouse Interleukin 31(IL-31) ELISA Kit

  • 中文名称：
    小鼠白介素31(IL-31)酶联免疫试剂盒
  • 货号：
    CSB-E13660m
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse IL31 ELISA Kit was designed for the quantitative measurement of Mouse IL31 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL .

  • 别名：
    Il31Interleukin-31 ELISA Kit; IL-31 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    31.25 pg/mL-2000 pg/mL
  • 灵敏度：
    7.81 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-31 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %90
    Range %82-99
    1:2Average %97
    Range %87-105
    1:4Average %95
    Range %88-100
    1:8Average %90
    Range %82-98
  • 回收率：
    The recovery of mouse IL-31 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9793-104
    EDTA plasma (n=4)9990-106
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20002.886 2.846 2.866 2.702
    10002.347 2.336 2.342 2.178
    5001.559 1.602 1.581 1.417
    2500.897 0.878 0.888 0.724
    1250.532 0.557 0.545 0.381
    62.50.366 0.374 0.370 0.206
    31.250.262 0.245 0.254 0.090
    00.162 0.165 0.164 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. May function in skin immunity. Enhances myeloid progenitor cell survival in vitro. Induces RETNLA and serum amyloid A protein expression in macrophages.
  • 基因功能参考文献：
    1. The serum levels of IL-33 and IL-31 were significantly up-regulated in both AR and allergic asthma patients compared with normal individuals. PMID: 30301503
    2. EPAS1 links DOCK8 deficiency to atopic skin inflammation via IL-31 induction in CD4-positive T cells. PMID: 28067314
    3. IL-31 increased the concentration of intracellular Ca(2+) ions in mouse keratinocytes. PMID: 29477033
    4. IL-31 elicits itch not only through direct action on primary sensory neurones, but also by inducing LTB4 production in keratinocytes. PMID: 28512667
    5. IL31 and its receptor, IL31RA, are highly expressed in various human and mouse cancer cell lines, as well as in tumor specimens from cancer patients. MC38 murine colon carcinoma cells depleted of IL31 exhibit an increase in invasive and migratory properties in vitro. PMID: 28147314
    6. Oncostatin M and interleukin-31: Cytokines, receptors, signal transduction and physiology. PMID: 26198770
    7. IL-31 upregulates IL-31RA expression in DRG neuron cell bodies, and cutaneous-injected IL-31-induced itching is enhanced by DRG IL-31RA expression in mice PMID: 25381841
    8. our results suggest a functional cooperation between NFAT1 and JunB in mediating IL-31 gene expression in CD4(+) T cells PMID: 25595785
    9. Data not only illustrate various functions that cysteines perform during IL-31 biosynthesis and secretion, but also highlight their potential roles in cytokine effector functions. PMID: 21182835
    10. IL-31 may be involved in promoting the dermatitis and epithelial responses that characterize allergic and non-allergic diseases PMID: 15184896
    11. IL-31 interaction with IL-31 receptor (IL-31R) is identified in an acute model of T helper cell type 2 inflammation in the lung. PMID: 17353366
    12. -31 antibody reduces scratching behaviour in an atopic dermatitis-like murine model during the onset of clinical skin manifestations PMID: 19054054
    13. these data provide the first evidence of a function for IL-31-IL-31R interactions in limiting the magnitude of type 2 inflammatory responses within the intestine. PMID: 19414760

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  • 亚细胞定位：
    Secreted.
  • 数据库链接：