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  4. Mouse Interleukin 1β,IL-1β ELISA Kit

Mouse Interleukin 1β,IL-1β ELISA Kit

  • 中文名称：
    小鼠白介素1β (IL-1β)酶联免疫试剂盒
  • 货号：
    CSB-E08054m
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：
        IL1B (白细胞介素-1β，Interleukin-1β) 是由许多不同类型的细胞产生的促炎症细胞因子，包括单核细胞、巨噬细胞、树突状细胞、T细胞和B细胞等。IL1B 参与了许多生理和病理过程，包括炎症、感染、肿瘤、自身免疫疾病等。
        j9九游会登录入口首页生物所提供的Mouse Interleukin 1β,IL-1β ELISA Kit属于ELISA检测试剂盒，采用双抗夹心法定量检测鼠血清、血浆、细胞培养上清、组织匀浆样本中的IL1B，其灵敏度为7.8 pg/mL，检测范围为31.25 pg/mL-2000 pg/mL。
     
  • 别名：
    Interleukin-1 beta ELISA Kit; IL-1 beta ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%  
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%  
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     
    　 Sample Serum(n=4)  
    1:1 Average % 88  
      Range % 82-95  
    1:2 Average % 93  
      Range % 88-98  
    1:4 Average % 96  
      Range % 94-102  
    1:8 Average % 94  
      Range % 90-100
     
  • 回收率：
    The recovery of mouse IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
     
    Sample Type Average % Recovery Range  
    Serum (n=5)   94 87-99  
    EDTA plasma (n=4) 93 88-98
     
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    2000 2.758 2.641 2.700 2.586  
    1000 1.438 1.587 1.513 1.399  
    500 0.833 0.869 0.851 0.737  
    250 0.474 0.477 0.476 0.362  
    125 0.350 0.375 0.363 0.249  
    62.5 0.267 0.253 0.260 0.146  
    31.25 0.165 0.186 0.176 0.062  
    0 0.119 0.108 0.114 　
     
  • 本试剂盒所含材料：
      • A micro ELISA plate --- The 96-well plate has been pre-coated with anti-mouse IL-1β This dismountable microplate can be divided into 12 x 8 strip plates.
      • Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • Biotin-labeled IL-1β antibody (100 x concentrate) 1 x 120 μl ---Act as the detection antibody.
      • HRP-avidin (100 x concentrate) 1 x 120 μl --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • Biotin-antibody Diluent 1 x 15 ml ---Dilute the high concentration Biotin-antibody to an appropriate working solution.
      • HRP-avidin Diluent 1 x 15 ml ---Dilute the high concentration HRP-avidin solution to an appropriate solution.
      • Sample Diluent 1 x 50 ml---Dilute the sample to an appropriate concentration.
      • Wash Buffer (25 x concentrate) 1 x 20 ml --- Wash away unbound or free substances.
      • TMB Substrate 1 x 10 ml --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • Stop Solution 1 x 10 ml --- Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

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  • 本试剂盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

平均分:
5.0分 - 4 个评价

样品类型：组织匀浆液 海马组织

样品信息：小鼠

稀释比：1:50

产品评价： 我用CSB-E08054m分别检测了造模及治疗前后小鼠海马组织匀浆中IL-1β水平，标曲结果良好，发现造模后IL-1β水平升高，磁刺激治疗后IL-1β水平降低，与预期结果一致。试剂盒操作方便，步骤简单便捷，节省了实验时间。

By 王老师

样品类型：组织匀浆液 肺组织匀浆上清

样品信息：小鼠

稀释比：没有稀释

产品评价： 我通过使用CSB-E08054m检测了PR8病毒感染的小鼠肺组织匀浆上清，操作简单方便易上手，灵敏度非常高，检测结果十分精准

By 邓老师

样品类型：血清

样品信息：小鼠

稀释比：没有稀释

产品评价： 我通过使用CSB-E08054m检测了流感病毒感染的小鼠外周血上清，操作简单方便易上手，灵敏度非常高，检测结果十分精准

By 邓老师

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问答及客户评论

■ 常见问题解答
Q:

I need a Elisa analysis of Interleukins IL 1beta on macrophase cell and lymphocytes.

A:
Thanks for your inquiry.
Regarding elisa kit for mouse Interleukin 1β,we have the following kit for you:
#CSB-E08054m,Mouse Interleukin 1β,IL-1β ELISA Kit
This kit is suitable to detect cell culture supernates.I will send you the kit manual by email.
But for macrophase cell and lymphocytes, sorry we did not test either of them with this kit.We suggest you do pre-testing if you want to test macrophase cell and lymphocytes.

靶点详情

  • 最新研究进展：
    IL-1β是一种炎症性细胞因子，主要参与机体的免疫和炎症反应。最近的研究表明，IL-1β在许多疾病中扮演着重要的角色，如自身免疫性疾病、炎症性肠病、关节炎等。此外，一些药物的研发也与IL-1β有关，如抗IL-1β单克隆抗体已被用于治疗风湿性关节炎等疾病。
  • 功能：
    Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6.
  • 基因功能参考文献：
    1. propofol was the most potent inhibitor of IL-1beta secretion and ROS level in S. aureus-infected RAW264.7 cells; moreover, propofol resulted in an increase in bacterial survival by inhibiting ROS and phagocytosis. PMID: 29667111
    2. P7 is an intracellular proton-gated H +-channel of the hepatitis C virus . P7 activity induced production of interleukin IL-1beta in liver macrophages. PMID: 27979709
    3. These results indicate that in RGCs, ANXA1 increases IL-1beta expression by recruiting p65 to the nucleus, which induces cell apoptosis. The obtained results may help the development of a novel treatment strategy against RGCs apoptosis in acute ischemia-reperfusion injury. PMID: 28389361
    4. Macrophage-derived IL1B/NF-kappaB signaling mediates parenteral nutrition-associated cholestasis in a mouse model. PMID: 29643332
    5. fenretinide impaired proinflammatory cytokine interleukin 1 beta (IL-1beta) production in response to A. fumigatus exposure with contributions by lectin-type oxidized LDL receptor 1 (LOX-1) and c-Jun N-terminal kinase (JNK). PMID: 30211745
    6. observation from the present research work reveals that Quercetin suppressed the production of proinflammatory cytokines at different levels, such as TNF-alpha and IL-1beta, and inhibits the activation of I-kappaB phosphorylation, whereas the total content was not affected. PMID: 29322353
    7. Overall, the s showed that CCN1 increased IL-1beta production via p38 MAPK signaling, indicating a role for CCN1 protein in regulating inflammation in psoriasis. PMID: 28266627
    8. In fibrocystin/polyductin complex-defective cholangiocytes, beta-catenin and IL-1beta are responsible for signal transducer and activator of transcription 3-dependent secretion of CXCL10 PMID: 29140564
    9. These results suggest that mitochondrial ROS-TXNIP/NLRP3/IL-1beta axis activation is responsible for tubular oxidative injury, which can be ameliorated by MitoQ via the inhibition of mtROS overproduction PMID: 29475133
    10. Circadian clock protein BMAL1 regulates IL-1beta in macrophages via NRF2. PMID: 30127006
    11. TLR2 and NLRP3 inflammasome activation in cardiac macrophages mediate the production of IL-1beta in diabetic mice. IL-1beta causes prolongation of the action potential duration, induces a decrease in potassium current and an increase in calcium sparks in cardiomyocytes, which are changes that underlie arrhythmia propensity. PMID: 27882934
    12. show that mutant KRAS facilitates IKKalpha-mediated responsiveness of tumor cells to host IL-1beta, thereby establishing a host-to-tumor signaling circuit that culminates in inflammatory MPE development and drug resistance PMID: 29445180
    13. identify interleukin-1 beta as an upstream trigger for the upregulation of interactions between USP5 and Cav3.2 channels in the pain pathway PMID: 28741432
    14. The present study demonstrated that IL-1b may induce ICAM-1 expression, thus enhancing the cohesion between mesenchymal stem cells and endothelial progenitor cells via the p38 MAPK signaling pathway. PMID: 29393395
    15. SAG2A differentially modulates IL-1beta expression in resistant and susceptible murine peritoneal macrophages cells. PMID: 29353306
    16. High IL-1beta expression is associated with experimental autoimmune encephalomyelitis. PMID: 29358392
    17. tibias of botulin A toxin-treated and tail-suspended mice, which featured unloading and decreased bone mass, showed higher expression of IL-1beta, Lcn2 and Nos2, suggesting their pathophysiologic involvement in endothelial cell-osteoblast crosstalk. PMID: 27430980
    18. HMGB1/IL-1beta complexes released after burn injuries can modulate immune responses PMID: 29601597
    19. bone marrow-derived macrophages (BMM) and three murine macrophage cell lines, J774.1, J774A.1, and RAW264.7 were exposed to ATP or fibrous titanium dioxide (FTiO2) in the presence or absence of lipopolysaccharide (LPS), and the concentrations of IL-1beta and IL-6 in both cell lysates and in the culture media were measured by immunoblotting to differentiate active form of IL-1beta from pro-IL-1beta. PMID: 28766178
    20. The present study demonstrates a novel mechanism underlying LPS-induced innate immunity; that is, a secondary upregulation of IL-1beta-IL-1RI signaling is responsible for alveolar macrophages pyroptosis and augmented lung injury in response to LPS. PMID: 27526865
    21. IL-33 may induce Th17 cell responses via IL-1beta and IL-6 derived from IL-33-matured dendritic cells. PMID: 28802996
    22. ESP of fifth-stage larval Angiostrongylus cantonensis stimulates astrocyte activation and IL-1beta and IL-6 production through NF-kappaB and the Shh signaling pathway. PMID: 28950910
    23. we confirmed that Th1 cell-conditioned medium decreased Cx43 protein levels in mixed glial cell cultures. These findings suggest that Th1 cell-derived IFNg activates microglia to release IL-1b that reduces Cx43 gap junctions in astrocytes. Thus, Th1-dominant inflammatory states disrupt astrocytic intercellular communication and may exacerbate multiple sclerosis. PMID: 27929069
    24. This finding shows that autophagy and NLRP3 inflammasome activation are connected, and that PTPN22 plays a key role in the regulation of those 2 pathways. PMID: 28786745
    25. These data suggest that amyloid formation leads to reduced PKB phosphorylation in beta-cells which is associated with elevated islet IL-1beta levels. Inhibitors of amyloid or amyloid-induced IL-1beta production may provide a new approach to restore phospho-PKB levels thereby enhance beta-cell survival and proliferation in conditions associated with islet amyloid formation PMID: 29474443
    26. Mice treated with HW for 4 weeks demonstrated a significant decrease in the AD severity score compared with PW-treated mice (p less than 0.01). Hydrogen water administration also significantly reduced TEWL and serum TARC levels (p less than 0.01), infiltration of mast cells (p less than 0.05), and secretion of the proinflammatory cytokines interleukin (IL)-1beta and IL-33 (p less than 0.05) in skin lesions compared wit... PMID: 28889151
    27. Curcumin attenuated neuropathic pain and down-regulated the production of spinal mature IL-1beta by inhibiting the aggregation of NALP1 inflammasome and the activation of the JAK2-STAT3 cascade in astrocytes. PMID: 27381056
    28. results altogether demonstrate distinct roles of SHARPIN in initiating systemic inflammation and dermatitis. Furthermore, skin inflammation in Sharpin(cpdm) mice is specifically modulated by IL-1beta, highlighting the importance of specific targeted therapies in the IL-1 signaling blockade. PMID: 27892465
    29. Food-grade synthetic amorphous silica particles are able to directly initiate the endosomal MyD88-dependent pathogen pattern recognition and signaling pathway in steady-state dendritic cells. The ensuing activation of immature DCs results in de novo induction of pro-IL-1beta. PMID: 28645296
    30. the present study confirmed that miR98 targeted the 3'untranslated region of Bcl2. In conclusion, miRNAcoordinated regulation of apoptosisassociated protein expression has been identified in Osteoarthritis chondrocytes following IL1beta induction. PMID: 28765925
    31. Results provide evidence that IL-1beta does not contribute to the pathophysiology of doxorubicin-induced cardiotoxicity. PMID: 27225830
    32. Alendronate (ALN)-augmented IL-1beta production and cell death require Smad3 and ASC activation, and SIS3 and anti-ASC antibodies may serve as palliative agents for necrotizing inflammatory diseases caused by ALN PMID: 29438662
    33. urinary LRG is produced in renal tubular epithelial cells by interleukin-1beta (IL-1beta) that is released during proteinuria-induced renal damage PMID: 29550485
    34. These data reveal how, upon XIAP deficiency, a TLR-TNF-TNFR2 axis drives cIAP1-TRAF2 degradation to allow TLR or TNFR1 activation of RIPK3-caspase-8 and IL-1beta. This mechanism may explain why XIAP-deficient patients can exhibit symptoms reminiscent of patients with activating inflammasome mutations. PMID: 28723569
    35. IL-1beta exerts variable effects on long-term potentiation at different kinds of synapses, indicating that IL-1beta has synapse-specific effects on hippocampal synaptic plasticity. PMID: 28637953
    36. we assessed the role of RIP3 in synergy with Caspase-1 in the induction of IL-1beta production in BMDM after either LPS/ATP or Chlamydia muridarum stimulation. The possibility of pyroptosis and necroptosis interplays and the role of RIP3 in IL-1beta production during Chlamydia muridarum infection in BMDM was investigated as well. PMID: 28660207
    37. Inhibition of signaling stimulated by both TNF and IL1beta synergizes with NF-kappaB inhibition in eliminating leukemic stem cells. PMID: 28039479
    38. Parenchymal polymorphonuclear myeloid-derived suppressor cell (PMN-MDSC), have a positive correlation with IL1a, IL8, CXCL5, and Mip-1a, suggesting they may attract PMN-MDSC into the tumor PMID: 27799249
    39. Chemokine receptor 2 (CCR2(+)) monocytes invade the hippocampus between 1 and 3 d after SE. In contrast, only an occasional CD3(+) T lymphocyte was encountered 3 d after SE. The initial cellular sources of the chemokine CCL2, a ligand for CCR2, included perivascular macrophages and microglia. The induction of the proinflammatory cytokine IL-1beta was greater in FACS-isolated microglia than in brain-invading monocytes PMID: 27601660
    40. hypernociception in experimental model of autoimmune encephalomyelitis may be a consequence of the increase in some cytokines in dorsal root ganglia, especially IL-1beta. PMID: 26614512
    41. An OA model was established in mouse articular chondrocytes (MACs) treated by interleukin-1beta (IL-1beta). PMID: 29247798
    42. The current study demonstrated that honey can stimulate or suppress the mRNA expression of some pro-inflammatory cytokines in mice brains. Furthermore, honey suppresses the TNF-alpha mRNA expression in the presence of T. gondii infection but it stimulates the IL-1beta and IL-6 mRNA expression. Treatment of the mice with honey reduces parasite multiplication in the brain. PMID: 27591508
    43. IL-1beta has a direct effect on NGAL production by tubular epithelial cells. PMID: 27997859
    44. Elevations of CO2 cause oligomerization of the inflammasome components ASC, NLRP3, caspase 1, thioredoxin interacting protein, and calreticulin - a protein from endoplasmic reticulum, leading to IL-1beta synthesis. An increased production rate of MPs containing elevated amounts of IL-1beta persists for hours after short-term exposures to elevated CO2 PMID: 28288918
    45. dimerized or endogenous caspase-8 can also directly cleave IL-1beta into its biologically active form, in the absence of canonical inflammasome components. PMID: 27419363
    46. In this newborn mouse lung hypoxia-reoxygenation model, we found downregulation of genes of mediators of inflammation, an antiapoptotic gene expression pattern, and downregulation of DNA glycosylases. Sod1 and Il1b were significantly differentially expressed when comparing reoxygenation using 60% O2 with air. PMID: 27529351
    47. Report direct role of pleural cells in the pathogenesis of bleomycin-induced pulmonary fibrosis via caspase-1/IL-1beta pathway. PMID: 27894300
    48. the senescence associated secretory phenotype was also increased significantly in the kidney of Sod1(-/)(-) mice compared to WT mice as measured by the expression of transcripts for IL-6 and IL-1b PMID: 27846439
    49. These studies elucidate an important role for neutrophils and IL-1beta in lung carcinogenesis. PMID: 27320908
    50. PLCd1 negatively regulates lipopolysaccharide-induced production of IL-1b and Fc gamma receptor-mediated phagocytosis in macrophages. PMID: 26643908

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  • 亚细胞定位：
    Cytoplasm, cytosol. Lysosome. Secreted, extracellular exosome. Cytoplasmic vesicle, autophagosome. Secreted.
  • 蛋白家族：
    IL-1 family
  • 组织特异性：
    Expressed in activated macrophages (at protein level).
  • 数据库链接：